Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Giberti, S.; Funck, D.; Forlani, G.
    delta1-Pyrroline-5-carboxylate reductase from Arabidopsis thaliana: stimulation or inhibition by chloride ions and feedback regulation by proline depend on whether NADPH or NADH acts as co-substrate (2014), New Phytol., 202, 911-919.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.2 expression in Esherichia coli Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.2 KCl inhibitory on NADH-dependent reaction Arabidopsis thaliana
1.5.1.2 additional information not inhibitory: NAD+ Arabidopsis thaliana
1.5.1.2 NaCl inhibitory on NADH-dependent reaction Arabidopsis thaliana
1.5.1.2 NADP+
-
 Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.2 0.026
-
 NADPH pH 7.8, 35°C Arabidopsis thaliana
1.5.1.2 0.096
-
 1-pyrroline-5-carboxylate cofactor NADPH, pH 7.8, 35°C Arabidopsis thaliana
1.5.1.2 0.21
-
 NADH pH 7.8, 35°C Arabidopsis thaliana
1.5.1.2 0.915
-
 1-pyrroline-5-carboxylate cofactor NADH, pH 7.8, 35°C Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.1.2 KCl stimulation of NADPH-dependent reaction, 70% stimulation at 100 mM Arabidopsis thaliana
1.5.1.2 NaCl stimulation of NADPH-dependent reaction, 70% stimulation at 100 mM Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.2 28000
-
-
 Arabidopsis thaliana
1.5.1.2 354000
-
 gel filtration Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.2 Arabidopsis thaliana F4K884
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.2 callus cell suspension culture established from cotyledon-derived calli Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.2 1-pyrroline-5-carboxylate + NADH + H+
-
 Arabidopsis thaliana L-proline + NAD+
-
 ?
1.5.1.2 1-pyrroline-5-carboxylate + NADPH + H+
-
 Arabidopsis thaliana L-proline + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.2 dodecamer 12 * 28000, SDS-PAGE Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.2 694
-
 NADPH pH 7.8, 35°C Arabidopsis thaliana
1.5.1.2 3049
-
 NADH pH 7.8, 35°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.2 7.3
-
 cofactor NADH Arabidopsis thaliana
1.5.1.2 8.4
-
 cofactor NADPH Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.2 NADH
-
 Arabidopsis thaliana
1.5.1.2 NADPH
-
 Arabidopsis thaliana

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.5.1.2 Arabidopsis thaliana isoelectric focusing
-
 4.7

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.5.1.2 0.032
-
 cofactor NADH, pH 7.8, 35°C Arabidopsis thaliana NADP+
1.5.1.2 1.44
-
 cofactor NADPH, pH 7.8, 35°C Arabidopsis thaliana NADP+
1.5.1.2 200
-
 cofactor NADH, pH 7.8, 35°C Arabidopsis thaliana NaCl
1.5.1.2 200
-
 cofactor NADH, pH 7.8, 35°C Arabidopsis thaliana KCl