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Literature summary extracted from

  • Tumbale, P.; Appel, C.D.; Kraehenbuehl, R.; Robertson, P.D.; Williams, J.S.; Krahn, J.; Ahel, I.; Williams, R.S.
    Structure of an aprataxin-DNA complex with insights into AOA1 neurodegenerative disease (2011), Nat. Struct. Mol. Biol., 18, 1189-1195.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.71 structure of APTX in complex with DNA, AMP and Zn2+ reveals active site and DNA interaction clefts formed by fusing a histidine triad nucleotide hydrolase with an DNA minor groove binding C2HE Zn-finger. An Aptx helical wedge interrogates the base stack for DNA end/nick sensing. Histidine triad nucleotide hydrolase, Zn-finger, the wedge, and an [F/Y]PK pivot motif cooperate to distort terminal DNA base-pairing and direct 5'-AMP into the active site pocket Schizosaccharomyces pombe

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.71 H138A mutation significantly impairs deadenylation activity Schizosaccharomyces pombe
3.6.1.71 H147N complete loss of activity Schizosaccharomyces pombe
3.6.1.71 S168A mutation significantly impairs deadenylation activity Schizosaccharomyces pombe

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.71 Schizosaccharomyces pombe O74859
-
-
3.6.1.71 Schizosaccharomyces pombe ATCC 24843 O74859
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.71 adenosine-5'-diphospho-5'-[DNA] + H2O
-
 Schizosaccharomyces pombe AMP + phospho-5'-[DNA]
-
 ?
3.6.1.71 adenosine-5'-diphospho-5'-[DNA] + H2O
-
 Schizosaccharomyces pombe ATCC 24843 AMP + phospho-5'-[DNA]
-
 ?
3.6.1.71 additional information mechanism involves nucleophilic attack of DNA 5'-adenylate by His147 to generate a covalent enzyme-AMP intermediate. His147 is stabilized by a close hydrogen bond with the Asn145 main chain carbonyl. The Aptx-specific His168-Ser168 pair appears positioned to activate solvent for hydrolysis of the enzyme-AMP intermediate. His168 may also act as a proton donor to the bridging oxygen in the first step Schizosaccharomyces pombe ?
-
 ?
3.6.1.71 additional information mechanism involves nucleophilic attack of DNA 5'-adenylate by His147 to generate a covalent enzyme-AMP intermediate. His147 is stabilized by a close hydrogen bond with the Asn145 main chain carbonyl. The Aptx-specific His168-Ser168 pair appears positioned to activate solvent for hydrolysis of the enzyme-AMP intermediate. His168 may also act as a proton donor to the bridging oxygen in the first step Schizosaccharomyces pombe ATCC 24843 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.71 aprataxin-like protein
-
 Schizosaccharomyces pombe
3.6.1.71 HNT3
-
 Schizosaccharomyces pombe