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Literature summary extracted from

  • Frandsen, K.E.; Simmons, T.J.; Dupree, P.; Poulsen, J.C.; Hemsworth, G.R.; Ciano, L.; Johnston, E.M.; Tovborg, M.; Johansen, K.S.; von Freiesleben, P.; Marmuse, L.; Fort, S.; Cottaz, S.; Driguez, H.; Henrissat, B.; Lenfant, N.; Tuna, F.; Baldansuren, A.; Davies, G.J.; Lo Leggio, L.; Walton, P.H.
    The molecular basis of polysaccharide cleavage by lytic polysaccharide monooxygenases (2016), Nat. Chem. Biol., 12, 298-303.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.56 expression in Aspergillus oryzae Panus similis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.99.56 substrate cellohexaose binds to subsites ?4 to +2, and cellotriose from subsites -1 to +2. Residue Tyr203 is placed approximately 15 A away from the copper active site Panus similis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.99.56 0.049
-
 cellotetraose pH 7.0, 37°C Panus similis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.99.56 Cu2+ residue Tyr203 is placed approximately 15 A away from the copper active site Panus similis

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.56 Panus similis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.56 cellohexaose + acceptor + O2
-
 Panus similis oxidized cellobiose and cellotriose + reduced acceptor + H2O
-
 ?
1.14.99.56 cellotetraose + acceptor + O2
-
 Panus similis ? + reduced acceptor + H2O
-
 ?
1.14.99.56 cellotriose + acceptor + O2
-
 Panus similis ? + reduced acceptor + H2O
-
 ?
1.14.99.56 phosphoric acid swollen cellulose + acceptor + O2
-
 Panus similis oxidized cellobiose and cellotriose + reduced acceptor + H2O main products, C4 is the sole site of oxidation ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.99.56 0.11
-
 cellotetraose pH 7.0, 37°C Panus similis