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Literature summary extracted from

  • Xue, Q.; Wei, Z.; Sun, W.; Cui, F.; Yu, S.; Zhou, Q.; Liu, J.
    2-Keto-D-gluconate-yielding membrane-bound D-glucose dehydrogenase from Arthrobacter globiformis C224: purification and characterization (2015), Molecules, 20, 846-862.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.5.2 n-hexane 92.1% increase of activity at 80% (v/v) Arthrobacter globiformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.5.2 Ca2+ slight inhibitory effect Arthrobacter globiformis
1.1.5.2 Cu2+ complete inhibition at 10 mM Arthrobacter globiformis
1.1.5.2 D-gluconic acid non-competitive inhibitor to D-glucose and competitive inhibitor to N-methylphenazonium methyl sulfate Arthrobacter globiformis
1.1.5.2 D-glucose
-
 Arthrobacter globiformis
1.1.5.2 EDTA complete inhibition at 0.5 mM Arthrobacter globiformis
1.1.5.2 ethanol 3.6% residual activity at 80% (v/v) Arthrobacter globiformis
1.1.5.2 Fe3+ complete inhibition at 10 mM Arthrobacter globiformis
1.1.5.2 methanol
-
 Arthrobacter globiformis
1.1.5.2 Mn2+ slight inhibitory effect Arthrobacter globiformis
1.1.5.2 N-methylphenazonium methyl sulfate
-
 Arthrobacter globiformis
1.1.5.2 Zn2+ slight inhibitory effect Arthrobacter globiformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.2 0.21
-
 D-glucose at pH 5.0 and 45°C Arthrobacter globiformis
1.1.5.2 0.34
-
 D-xylose at pH 5.0 and 45°C Arthrobacter globiformis
1.1.5.2 0.4
-
 N-methylphenazonium methyl sulfate with D-glucose as cosubstrate, at pH 5.0 and 45°C Arthrobacter globiformis
1.1.5.2 0.46
-
 D-galactose at pH 5.0 and 45°C Arthrobacter globiformis
1.1.5.2 0.59
-
 maltose at pH 5.0 and 45°C Arthrobacter globiformis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.2 membrane
-
 Arthrobacter globiformis 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.5.2 Mg2+ 20% increase of activity at 1 mM Arthrobacter globiformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.2 87000
-
 x * 87000, SDS-PAGE Arthrobacter globiformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.5.2 D-glucose + ubiquinone Arthrobacter globiformis preferred substrate D-glucono-1,5-lactone + ubiquinol
-
 ?
1.1.5.2 D-glucose + ubiquinone Arthrobacter globiformis C224 preferred substrate D-glucono-1,5-lactone + ubiquinol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.2 Arthrobacter globiformis
-
-
-
1.1.5.2 Arthrobacter globiformis C224
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.2 Triton X-114 phase precipitation, acetone precipitation, polyethylene glcyol 6000 precipitation, ethanol precipitation, and hydroxylapatite column chromatography Arthrobacter globiformis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.5.2 0.5
-
 crude extract, at pH 5.0 and 45°C Arthrobacter globiformis
1.1.5.2 88.1
-
 after 183.5fold purification, at pH 5.0 and 45°C Arthrobacter globiformis

Storage Stability

EC Number Storage Stability Organism
1.1.5.2 4°C, at pH 4.0 or 9.0, 2 days, complete loss of activity Arthrobacter globiformis
1.1.5.2 4°C, at pH 6.0-7.0, 2 days, no loss of activity Arthrobacter globiformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.2 D-arabinose + N-methylphenazonium methyl sulfate 8.0% activity compared to D-glucose Arthrobacter globiformis D-arabinono-1,5-lactone + ?
-
 ?
1.1.5.2 D-arabinose + N-methylphenazonium methyl sulfate 8.0% activity compared to D-glucose Arthrobacter globiformis C224 D-arabinono-1,5-lactone + ?
-
 ?
1.1.5.2 D-galactose + N-methylphenazonium methyl sulfate 20% activity compared to D-glucose Arthrobacter globiformis D-galactono-1,5-lactone + ?
-
 ?
1.1.5.2 D-galactose + N-methylphenazonium methyl sulfate 20% activity compared to D-glucose Arthrobacter globiformis C224 D-galactono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + ferricyanide D-glucose is the preferred substrate Arthrobacter globiformis D-glucono-1,5-lactone + ferrocyanide
-
 ?
1.1.5.2 D-glucose + ferricyanide D-glucose is the preferred substrate Arthrobacter globiformis C224 D-glucono-1,5-lactone + ferrocyanide
-
 ?
1.1.5.2 D-glucose + N-methylphenazonium methyl sulfate D-glucose is the preferred substrate Arthrobacter globiformis D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + ubiquinone preferred substrate Arthrobacter globiformis D-glucono-1,5-lactone + ubiquinol
-
 ?
1.1.5.2 D-glucose + ubiquinone preferred substrate Arthrobacter globiformis C224 D-glucono-1,5-lactone + ubiquinol
-
 ?
1.1.5.2 D-xylose + N-methylphenazonium methyl sulfate 22% activity compared to D-glucose Arthrobacter globiformis D-xylono-1,5-lactone + ?
-
 ?
1.1.5.2 maltose + N-methylphenazonium methyl sulfate 17% activity compared to D-glucose Arthrobacter globiformis maltono-1,5-lactone + ?
-
 ?
1.1.5.2 additional information no activity with D-gluconate, D-sorbose, D-mannose, D-fructose, malic acid, sucrose, and citric acid Arthrobacter globiformis ?
-
 ?
1.1.5.2 additional information no activity with D-gluconate, D-sorbose, D-mannose, D-fructose, malic acid, sucrose, and citric acid Arthrobacter globiformis C224 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.5.2 ? x * 87000, SDS-PAGE Arthrobacter globiformis
1.1.5.2 ? x * 87067, calculated from amino acid sequence Arthrobacter globiformis

Synonyms

EC Number Synonyms Comment Organism
1.1.5.2 GlcDH
-
 Arthrobacter globiformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.5.2 45
-
-
 Arthrobacter globiformis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.5.2 15 85 the enzyme reaches to the maximum activity with the increase of reaction temperature from 15 to 45 °C, and follows the sharp decline of activity to 30% at 55°C, and shows no activity at 85°C Arthrobacter globiformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.5.2 40 50 low temperature of below 40°C has the stable and highest enzyme activity after kept for 120 min. Increase of storage temperature to over 50°C inhibits the activity of above 58% after 10 min treatment, while no activity is detected after incubation for 60 min at 60 or 70°C Arthrobacter globiformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.2 5
-
-
 Arthrobacter globiformis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.5.2 4 6 the enzyme relative activity decreases from optimum at pH 5.0 to approximately 51% or 36% when the pH drops to 4.0 or increases to 6.0, respectively Arthrobacter globiformis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.5.2 11.4
-
 N-methylphenazonium methyl sulfate at pH 5.0 and 45°C Arthrobacter globiformis
1.1.5.2 14.8
-
 D-glucose at pH 5.0 and 45°C Arthrobacter globiformis