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Literature summary extracted from

  • Di Vona, C.; Bezdan, D.; Islam, A.B.; Salichs, E.; Lopez-Bigas, N.; Ossowski, S.; de la Luna, S.
    Chromatin-wide profiling of DYRK1A reveals a role as a gene-specific RNA polymerase II CTD kinase (2015), Mol. Cell, 57, 506-520.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.23 additional information DYRK1A expression is downregulated by lentiviral delivery of a DYRK1A-specific shRNA, reduction in expression of DYRK1A target genes in T98G and HeLa cells after DYRK1A knockdown with two shRNAs targeting different regions of DYRK1A Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.23 harmine
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.23 chromatin DYRK1A peaks overlapped with nucleosome-depleted regions Homo sapiens 785
-
2.7.11.23 cytoplasm
-
 Homo sapiens 5737
-
2.7.11.23 nucleus nuclear DYRK1A is associated with high molecular weight complexes that co-fractionate with RNAPII Homo sapiens 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.23 Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.23 ATP + [DNA-directed RNA polymerase] Homo sapiens DYRK1A phosphorylates the C-terminal domain of RNA polymerase II at Ser2 and Ser5. The mammalian RNAPII C-terminal domain consists of 52 hepta-residue repeats (YSPTSPS), and its phosphorylation governs the paused or elongating phases of the transcription cycle ADP + phospho-[DNA-directed RNA polymerase]
-
 ?
2.7.11.23 additional information Homo sapiens DYRK1A targets contain a TCTCGCGAGA motif, which drives DYRK1A-dependent activation. DYRK1A interacts with RNAPII independently of its kinase activity ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.23 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.23 glioblastoma cell
-
 Homo sapiens
-
2.7.11.23 HeLa cell
-
 Homo sapiens
-
2.7.11.23 T-98G cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.23 ATP + DYRKtide
-
 Homo sapiens ADP + phospho-DYRKtide
-
 ?
2.7.11.23 ATP + [DNA-directed RNA polymerase] DYRK1A phosphorylates the C-terminal domain of RNA polymerase II at Ser2 and Ser5. The mammalian RNAPII C-terminal domain consists of 52 hepta-residue repeats (YSPTSPS), and its phosphorylation governs the paused or elongating phases of the transcription cycle Homo sapiens ADP + phospho-[DNA-directed RNA polymerase]
-
 ?
2.7.11.23 ATP + [DNA-directed RNA polymerase] DYRK1A phosphorylates the C-terminal domain of RNA polymerase II at Ser2 and Ser5 Homo sapiens ADP + phospho-[DNA-directed RNA polymerase]
-
 ?
2.7.11.23 additional information DYRK1A targets contain a TCTCGCGAGA motif, which drives DYRK1A-dependent activation. DYRK1A interacts with RNAPII independently of its kinase activity Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.23 Down syndrome-associated kinase
-
 Homo sapiens
2.7.11.23 DYRK1A
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.23 ATP
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.7.11.23 malfunction DYRK1A depletion leads to reduced Ser2/Ser5 phosphorylation at its target promoters. The combination of the loss of RNAPII association to the target promoters together with the reduced CTD phosphorylation of the elongating RNAPII is responsible for the reduced expression of the target genes upon DYRK1A depletion Homo sapiens
2.7.11.23 metabolism DYRK1A is a dosage-sensitive protein kinase that fulfills key roles during development and in tissue homeostasis. DYRK1A-bound promoters show a higher occurrence of activating chromatin modifications when compared with genes that do not associate with DYRK1A, including high levels of H3K4me3 and H3K4me2 surrounding TSSs or elevated H3K36me3 in the gene body Homo sapiens
2.7.11.23 physiological function Down syndrome-associated kinase DYRK1A regulates transcription of a subset of genes by associating to their proximal promoter regions and phosphorylating the C-terminal domain of the RNA polymerase II. Protein kinase DYRK1A is recruited to proximal promoters of growth-related genes, genome-wide analysis, overview. DYRK1A recruitment to its target genes is independent of cell growth conditions and the enzyme is recruited to chromatin in both proliferating and resting cells. Enzyme DYRK1A shows ability to activate transcription when tethered to a minimal promoter, relationship between DYRK1A promoter occupancy and the transcriptional activity of its target genes, overview. The TCTCGCGAGA sequence is a regulatory motif in DYRK1A target promoters Homo sapiens