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Literature summary extracted from

  • Badjatia, N.; Ambrosio, D.L.; Lee, J.H.; Guenzl, A.
    Trypanosome cdc2-related kinase 9 controls spliced leader RNA cap4 methylation and phosphorylation of the RNA polymerase II subunit RPB1 (2013), Mol. Cell. Biol., 33, 1965-1975.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.23 gene CRK9, quantitative real-time reverse transcription-PCR expression analysis Trypanosoma brucei

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.23 additional information CRK9 silencing by expression of RNAi Trypanosoma brucei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.23 Mg2+ required Trypanosoma brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.23 ATP + [DNA-directed RNA polymerase] Trypanosoma brucei enzyme phosphorylates residues in the C-terminal domain, CTD, of the largest RNA polymerase II (RNA Pol II) subunit, RPB1 ADP + phospho-[DNA-directed RNA polymerase]
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Organism

EC Number Organism UniProt Comment Textmining
2.7.11.23 Trypanosoma brucei
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.23 ATP + [DNA-directed RNA polymerase] enzyme phosphorylates residues in the C-terminal domain, CTD, of the largest RNA polymerase II (RNA Pol II) subunit, RPB1 Trypanosoma brucei ADP + phospho-[DNA-directed RNA polymerase]
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 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.23 cdc2-related kinase 9
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 Trypanosoma brucei
2.7.11.23 CRK9
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 Trypanosoma brucei

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.23 ATP
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 Trypanosoma brucei

General Information

EC Number General Information Comment Organism
2.7.11.23 evolution protistan organisms, such as amoebozoa, kinetoplastids, trichomonads, and diplomonads, which have diverged very early from other eukaryotic lineages, lack repetitive CTD motifs Trypanosoma brucei
2.7.11.23 malfunction silencing the expression of Trypanosoma brucei cdc2-related kinase 9 (CRK9) leads to a loss of RPB1 phosphorylation, but that does not impair RNA Pol II transcription or cotranscriptional m7G capping. Instead, CRK9 silencing leads to a block of spliced leader (SL) trans splicing, an essential step in trypanosome mRNA maturation, that is caused by hypomethylation of the SL RNA's unique cap4. Trypanosoma brucei
2.7.11.23 physiological function human parasite Trypanosoma brucei lacks the heptad repeats and a CDK7 orthologue. Characterization of trypanosome TFIIH does not identify a kinase component. The Trypanosoma brucei CTD nevertheless is phosphorylated by cdc2-related kinase 9 (CRK9) and is essential for transcription. RPB1 phosphorylation and SL RNA cap4 formation depend on CRK9 kinase activity. CRK9-dependent RPB1 phosphorylation is neither required for transcription initiation at the SLRNA promoter nor an essential modification for transcription elongation within the protein coding gene arrays Trypanosoma brucei