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Literature summary extracted from

  • Rozova, O.N.; Khmelenina, V.N.; Bocharova, K.A.; Mustakhimov, I.I.; Trotsenko, Y.A.
    Role of NAD+-dependent malate dehydrogenase in the metabolism of Methylomicrobium alcaliphilum 20Z and Methylosinus trichosporium OB3b (2015), Microorganisms, 3, 47-59.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.37 additional information the thermostable enzyme is not affected by metal ions (CuCl2, MgCl2, MnCl2, CoCl2, BaCl2, ZnCl2 or CaCl2 and NaCl or KCl) or various organic metabolites (pyruvate, phosphoenolpyruvate, ATP, ADP, AMP, glucose-1-phospate, fructose-1-phosphate, fructose-1,6-bisphosphate, fructose-6-phosphate, ribose-1-phosphate, ribose-5-phosphate (all 5 mM), glycerate, lactate, 2-oxoglutarate, citrate, serine, diphosphate (all 1 mM) or phosphate (10 mM)) Methylotuvimicrobium alcaliphilum
1.1.1.37 additional information the thermostable enzyme is not affected by metal ions (CuCl2, MgCl2, MnCl2, CoCl2, BaCl2, ZnCl2 or CaCl2 and NaCl or KCl) or various organic metabolites (Pyruvate, phosphoenolpyruvate, ATP, ADP, AMP, glucose-1-phospate, fructose-1-phosphate, fructose-1,6-bisphosphate, fructose-6-phosphate, ribose-1-phosphate, ribose-5-phosphate (all 5 mM), glycerate, lactate, 2-oxoglutarate, citrate, serine, pyrophosphate (all 1 mM) or ?H2PO4 (10 mM)) Methylosinus trichosporium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.37 gene mdh, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Methylosinus trichosporium
1.1.1.37 gene mdh, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Methylotuvimicrobium alcaliphilum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.37 additional information the thermostable enzyme is not affected by metal ions (CuCl2, MgCl2, MnCl2, CoCl2, BaCl2, ZnCl2 or CaCl2 and NaCl or KCl) or various organic metabolites (pyruvate, phosphoenolpyruvate, ATP, ADP, AMP, glucose-1-phospate, fructose-1-phosphate, fructose-1,6-bisphosphate, fructose-6-phosphate, ribose-1-phosphate, ribose-5-phosphate (all 5 mM), glycerate, lactate, 2-oxoglutarate, citrate, serine, diphosphate (all 1 mM) or phosphate (10 mM)) Methylosinus trichosporium
1.1.1.37 additional information the thermostable enzyme is not affected by metal ions (CuCl2, MgCl2, MnCl2, CoCl2, BaCl2, ZnCl2 or CaCl2 and NaCl or KCl) or various organic metabolites (Pyruvate, phosphoenolpyruvate, ATP, ADP, AMP, glucose-1-phospate, fructose-1-phosphate, fructose-1,6-bisphosphate, fructose-6-phosphate, ribose-1-phosphate, ribose-5-phosphate (all 5 mM), glycerate, lactate, 2-oxoglutarate, citrate, serine, pyrophosphate (all 1 mM) or ?H2PO4 (10 mM)) Methylotuvimicrobium alcaliphilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.37 additional information
-
 additional information Michaelis-Menten kinetics Methylosinus trichosporium
1.1.1.37 additional information
-
 additional information Michaelis-Menten kinetics Methylotuvimicrobium alcaliphilum
1.1.1.37 0.0252
-
 NADH recombinant enzyme, pH 10.0, 30°C Methylotuvimicrobium alcaliphilum
1.1.1.37 0.0376
-
 NADH recombinant enzyme, pH 9.5, 30°C Methylosinus trichosporium
1.1.1.37 0.059
-
 oxaloacetate recombinant enzyme, pH 9.5, 30°C Methylosinus trichosporium
1.1.1.37 0.11
-
 (S)-malate recombinant enzyme, pH 10.0, 30°C Methylotuvimicrobium alcaliphilum
1.1.1.37 0.33
-
 NAD+ recombinant enzyme, pH 9.5, 30°C Methylosinus trichosporium
1.1.1.37 0.34
-
 oxaloacetate recombinant enzyme, pH 10.0, 30°C Methylotuvimicrobium alcaliphilum
1.1.1.37 0.45
-
 NAD+ recombinant enzyme, pH 10.0, 30°C Methylotuvimicrobium alcaliphilum
1.1.1.37 1.28
-
 (S)-malate recombinant enzyme, pH 9.5, 30°C Methylosinus trichosporium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.37 additional information the thermostable enzyme is not affected by metal ions (CuCl2, MgCl2, MnCl2, CoCl2, BaCl2, ZnCl2 or CaCl2 and NaCl or KCl) or various organic metabolites (pyruvate, phosphoenolpyruvate, ATP, ADP, AMP, glucose-1-phospate, fructose-1-phosphate, fructose-1,6-bisphosphate, fructose-6-phosphate, ribose-1-phosphate, ribose-5-phosphate (all 5 mM), glycerate, lactate, 2-oxoglutarate, citrate, serine, diphosphate (all 1 mM) or phosphate (10 mM)) Methylosinus trichosporium
1.1.1.37 additional information the thermostable enzyme is not affected by metal ions (CuCl2, MgCl2, MnCl2, CoCl2, BaCl2, ZnCl2 or CaCl2 and NaCl or KCl) or various organic metabolites (pyruvate, phosphoenolpyruvate, ATP, ADP, AMP, glucose-1-phospate, fructose-1-phosphate, fructose-1,6-bisphosphate, fructose-6-phosphate, ribose-1-phosphate, ribose-5-phosphate (all 5 mM), glycerate, lactate, 2-oxoglutarate, citrate, serine, diphosphate (all 1 mM) or phosphate (10 mM)) Methylotuvimicrobium alcaliphilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.37 33200
-
-
 Methylosinus trichosporium
1.1.1.37 35000
-
-
 Methylosinus trichosporium
1.1.1.37 35000
-
-
 Methylotuvimicrobium alcaliphilum
1.1.1.37 35800
-
-
 Methylotuvimicrobium alcaliphilum
1.1.1.37 70000
-
 recombinant enzyme, gel filtration Methylotuvimicrobium alcaliphilum
1.1.1.37 140000
-
 recombinant enzyme, gel filtration Methylosinus trichosporium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.37 (S)-malate + NAD+ Methylosinus trichosporium
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+ Methylotuvimicrobium alcaliphilum
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+ Methylotuvimicrobium alcaliphilum 20Z
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 oxaloacetate + NADH + H+ Methylosinus trichosporium
-
 (S)-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH + H+ Methylotuvimicrobium alcaliphilum
-
 (S)-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH + H+ Methylotuvimicrobium alcaliphilum 20Z
-
 (S)-malate + NAD+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.37 Methylosinus trichosporium
-
-
-
1.1.1.37 Methylotuvimicrobium alcaliphilum G4T3S9
-
-
1.1.1.37 Methylotuvimicrobium alcaliphilum 20Z G4T3S9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.37 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Methylosinus trichosporium
1.1.1.37 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Methylotuvimicrobium alcaliphilum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.37 15.05
-
 purified recombinant enzyme, oxaloacetate reduction, pH 10.0, 30°C Methylotuvimicrobium alcaliphilum
1.1.1.37 20.75
-
 purified recombinant enzyme, malate oxidation, pH 10.0, 30°C Methylotuvimicrobium alcaliphilum
1.1.1.37 78.2
-
 purified recombinant enzyme, malate oxidation, pH 9.5, 30°C Methylosinus trichosporium
1.1.1.37 187.8
-
 purified recombinant enzyme, oxaloacetate reduction, pH 9.5, 30°C Methylosinus trichosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.37 (S)-malate + NAD+
-
 Methylosinus trichosporium oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Methylotuvimicrobium alcaliphilum oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Methylotuvimicrobium alcaliphilum 20Z oxaloacetate + NADH + H+
-
 r
1.1.1.37 additional information the enzyme displays nearly equal reductive (malate formation) and oxidative (oxaloacetate formation) activities and higher affinity to malate than to oxaloacetate Methylotuvimicrobium alcaliphilum ?
-
 ?
1.1.1.37 additional information the enzyme is 2fold more active in the reaction of oxaloacetate reduction compared to malate oxidation and exhibiting higher affinity to oxaloacetate than to malate Methylosinus trichosporium ?
-
 ?
1.1.1.37 additional information the enzyme displays nearly equal reductive (malate formation) and oxidative (oxaloacetate formation) activities and higher affinity to malate than to oxaloacetate Methylotuvimicrobium alcaliphilum 20Z ?
-
 ?
1.1.1.37 oxaloacetate + NADH + H+
-
 Methylosinus trichosporium (S)-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH + H+
-
 Methylotuvimicrobium alcaliphilum (S)-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH + H+
-
 Methylotuvimicrobium alcaliphilum 20Z (S)-malate + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.37 homodimer 2 * 35000, recombinant enzyme, SDS-PAGE, 2 * 35800, about, sequence calculation Methylotuvimicrobium alcaliphilum
1.1.1.37 homotetramer 4 * 35000, recombinant enzyme, SDS-PAGE, 4 * 33200, about, sequence calculation Methylosinus trichosporium

Synonyms

EC Number Synonyms Comment Organism
1.1.1.37 A8B73_11505
-
 Methylosinus trichosporium
1.1.1.37 L-malate: NAD oxidoreductase
-
 Methylosinus trichosporium
1.1.1.37 L-malate: NAD oxidoreductase
-
 Methylotuvimicrobium alcaliphilum
1.1.1.37 MDH
-
 Methylosinus trichosporium
1.1.1.37 MDH
-
 Methylotuvimicrobium alcaliphilum
1.1.1.37 NAD+-dependent malate dehydrogenase
-
 Methylosinus trichosporium
1.1.1.37 NAD+-dependent malate dehydrogenase
-
 Methylotuvimicrobium alcaliphilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.37 60
-
-
 Methylosinus trichosporium
1.1.1.37 60 65
-
 Methylotuvimicrobium alcaliphilum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.37 30 60 the activity of the methanotroph's enzyme increases up to 60°? and then gradually decreases Methylosinus trichosporium
1.1.1.37 30 60 the activity of the methanotroph's enzyme increases up to 60°? and then gradually decreases Methylotuvimicrobium alcaliphilum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.37 30 70 the enzyme is active in a broader temperature range. It retains full activity after 18 h exposure at 30-60°C, exhibiting a 1 h half-life time at 70°C Methylosinus trichosporium
1.1.1.37 30 60 the purified recombinant His6-tagged MDH from strain 20Z fully retains activity after 18 h exposure at 30-40°C, and has a 10% residual activity after 18 h exposure at 50°C. The enzyme loses 50% of its activity after 10 min heating at 60°C Methylotuvimicrobium alcaliphilum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.37 516
-
 oxaloacetate recombinant enzyme, pH 9.5, 30°C Methylosinus trichosporium
1.1.1.37 621
-
 (S)-malate recombinant enzyme, pH 9.5, 30°C Methylotuvimicrobium alcaliphilum
1.1.1.37 870
-
 oxaloacetate recombinant enzyme, pH 9.5, 30°C Methylotuvimicrobium alcaliphilum
1.1.1.37 1141
-
 (S)-malate recombinant enzyme, pH 9.5, 30°C Methylosinus trichosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.37 9.5
-
-
 Methylosinus trichosporium
1.1.1.37 10
-
-
 Methylotuvimicrobium alcaliphilum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.37 7.5 10 activity range, recombinant enzyme Methylosinus trichosporium
1.1.1.37 7.5 10 activity range, recombinant enzyme Methylotuvimicrobium alcaliphilum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.37 NAD+
-
 Methylosinus trichosporium
1.1.1.37 NAD+
-
 Methylotuvimicrobium alcaliphilum
1.1.1.37 NADH
-
 Methylosinus trichosporium
1.1.1.37 NADH
-
 Methylotuvimicrobium alcaliphilum

General Information

EC Number General Information Comment Organism
1.1.1.37 metabolism the enzyme plays crucial roles in many metabolic pathways, including the tricarboxylic acid (TCA) cycle, energy generation and the formation of metabolites for biosynthesis Methylosinus trichosporium
1.1.1.37 metabolism the enzyme plays crucial roles in many metabolic pathways, including the tricarboxylic acid (TCA) cycle, energy generation and the formation of metabolites for biosynthesis Methylotuvimicrobium alcaliphilum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.37 403
-
 (S)-malate recombinant enzyme, pH 9.5, 30°C Methylosinus trichosporium
1.1.1.37 2558
-
 oxaloacetate recombinant enzyme, pH 9.5, 30°C Methylotuvimicrobium alcaliphilum
1.1.1.37 5648
-
 (S)-malate recombinant enzyme, pH 9.5, 30°C Methylotuvimicrobium alcaliphilum
1.1.1.37 19340
-
 oxaloacetate recombinant enzyme, pH 9.5, 30°C Methylosinus trichosporium