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Literature summary extracted from

  • Ji, W.; Chen, Y.; Zhang, H.; Zhang, X.; Li, Z.; Yu, Y.
    Cloning, expression and characterization of a putative 7alpha-hydroxysteroid dehydrogenase in Comamonas testosteroni (2014), Microbiol. Res., 169, 148-154.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.159 gene 7alpha-HSD, located 11.9 kb upstream of the hsdA gene, DNA and amino acid sequence determination and analysis, phylogenetic analysis, subcloning in Escherichia coli strain HB101, sequence comparisons, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS Comamonas testosteroni

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.159 additional information construction of a 7alpha -HSD knockout mutant that shows decreased degradation of testosterone, estradiol and cholesterol compared to the wild-type Comamonas testosteroni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.159 27000
-
-
 Comamonas testosteroni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.159 cholate + NAD+ Comamonas testosteroni
-
 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H+
-
 r
1.1.1.159 cholate + NAD+ Comamonas testosteroni ATCC 11996
-
 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.159 Comamonas testosteroni S5U393 i.e. Pseudomonas testosteroni
-
1.1.1.159 Comamonas testosteroni ATCC 11996 S5U393 i.e. Pseudomonas testosteroni
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.159 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) pLysS by metal affinity chromatography Comamonas testosteroni

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.159 additional information Comamonas testosteroni strain ATCC11996 is a Gram-negative bacterium which can use steroids as carbon and energy source Comamonas testosteroni
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.159 cholate + NAD+
-
 Comamonas testosteroni 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H+
-
 r
1.1.1.159 cholate + NAD+
-
 Comamonas testosteroni ATCC 11996 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.159 ? x * 27000, recombinant His-tagged enzyme, SDS-PAGE Comamonas testosteroni

Synonyms

EC Number Synonyms Comment Organism
1.1.1.159 7alpha-HSD
-
 Comamonas testosteroni

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.159 NAD+ the enzyme sequence contains an N-terminal Gly-X-X-X-Gly-X-Gly cofactor-binding motif Comamonas testosteroni

General Information

EC Number General Information Comment Organism
1.1.1.159 evolution the enzyme is a member of the short-chain dehydrogenase/reductase (SDR) superfamily Comamonas testosteroni
1.1.1.159 malfunction a 7alpha -HSD knockout mutant shows decreased degradation of testosterone, estradiol and cholesterol compared to the wild-type Comamonas testosteroni
1.1.1.159 additional information the enzyme sequence contains a Tyr-X-X-X-Lys segment, residues 161-165, that is essential for catalytic activity of SDR proteins Comamonas testosteroni