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Literature summary extracted from

  • Zhang, R.; Wang, L.; Xu, Y.; Liang, H.; Zhou, X.; Jiang, J.; Li, Y.; Xiao, R.; Ni, Y.
    In situ expression of (R)-carbonyl reductase rebalancing an asymmetric pathway improves stereoconversion efficiency of racemic mixture to (S)-phenyl-1,2-ethanediol in Candida parapsilosis CCTCC M203011 (2016), Microb. Cell Fact., 15, 143.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.1.1.184 synthesis in situ expression in Candida leads to over fourfold higher activity for conversion of racemic (R,S)-1-phenyl-1,2-ethanediol to 2-hydroxyacetophenone, while maintaining the activity for catalyzing 2-hydroxyacetophenone to (S)-1-phenyl-1,2-ethanediol. A recombinant Candida parapsilosis converts racemic (R,S)-1-phenyl-1,2-ethanediol to its (S)-isomer with an optical purity of 98.8% and a yield of 48.4%. The biotransformation duration is reduced from 48 to 13 h Candida parapsilosis CCTCC M203011

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.184
-
 Candida parapsilosis CCTCC M203011

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.184 Candida parapsilosis CCTCC M203011 A1X808
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.184 (R)-1-phenyl-1,2-ethanediol + NADH + H+
-
 Candida parapsilosis CCTCC M203011 2-hydroxyacetophenone + NAD+
-
 r
1.1.1.184 hydroxyacetophenone + NAD+
-
 Candida parapsilosis CCTCC M203011 (R)-1-phenyl-1,2-ethanediol + NADH + H+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.184 30
-
 oxidation of (R)-1-phenyl-1,2-ethanediol Candida parapsilosis CCTCC M203011
1.1.1.184 35
-
 reduction of hydroxyacetophenone Candida parapsilosis CCTCC M203011

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.184 5
-
 oxidation of (R)-1-phenyl-1,2-ethanediol Candida parapsilosis CCTCC M203011
1.1.1.184 6.5
-
 reduction of hydroxyacetophenone Candida parapsilosis CCTCC M203011