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Literature summary extracted from

  • Liang, K.; Shen, C.R.
    Selection of an endogenous 2,3-butanediol pathway in Escherichia coli by fermentative redox balance (2017), Metab. Eng., 39, 181-191.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.304 gene budC, functional recombinant expression of NADH-dependent Kp-BudC from plasmid pKM3 in Escherichia coli wild-type strain BW25113 and in mutant strain JCL166 (BW25113/F' [traD36, proABþ, lacIqZ DELTAM15 (Tet)] JCL16 DELTAadhE DELTAldhA DELTAfrdBC) Klebsiella pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.304 additional information selection of an endogenous 2,3-butanediol pathway in Escherichia coli by fermentative redox balance, overview. Anaerobic growth rescue with feeding of acetoin of the deletion mutant strain JCL166 transformed with the designated plasmid carrying the Kp-BudC, Cb-Adh, or the Ec-GldA. substrate-decoupled selection platform based on anaerobic redox balance Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.4 3.4
-
 acetoin cosubstrate NADH, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.4 13.6
-
 acetoin cosubstrate NADPH, pH 7.0, temperature not specified in the publication Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.304 diacetyl + NADH + H+ Klebsiella pneumoniae
-
 (S)-acetoin + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.4 Escherichia coli
-
-
-
1.1.1.4 Escherichia coli BW25113
-
-
-
1.1.1.304 Klebsiella pneumoniae Q48436
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.4 acetoin + NADH + H+
-
 Escherichia coli (R,R)-butanediol + NAD+
-
 ?
1.1.1.4 acetoin + NADH + H+
-
 Escherichia coli BW25113 (R,R)-butanediol + NAD+
-
 ?
1.1.1.4 acetoin + NADPH + H+
-
 Escherichia coli (R,R)-butanediol + NADP+
-
 ?
1.1.1.4 acetoin + NADPH + H+
-
 Escherichia coli BW25113 (R,R)-butanediol + NADP+
-
 ?
1.1.1.304 diacetyl + NADH + H+
-
 Klebsiella pneumoniae (S)-acetoin + NAD+
-
 ?
1.1.1.304 diacetyl + NADH + H+ stereospecific reaction Klebsiella pneumoniae (S)-acetoin + NAD+
-
 ?
1.1.1.304 additional information the enzyme BudC also catalyzes the reduction of acetoin by NADH, cf. EC 1.1.1.76 Klebsiella pneumoniae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.304 budC
-
 Klebsiella pneumoniae
1.1.1.304 DAR
-
 Klebsiella pneumoniae
1.1.1.304 diacetyl reductase
-
 Klebsiella pneumoniae
1.1.1.304 Kp-BudC
-
 Klebsiella pneumoniae
1.1.1.304 Kpdar
-
 Klebsiella pneumoniae
1.1.1.304 NADH-dependent Kp-BudC
-
 Klebsiella pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.4 0.5
-
 acetoin cosubstrate NADPH, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.4 1.4
-
 acetoin cosubstrate NADH, pH 7.0, temperature not specified in the publication Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.4 NADH preferred over NADPH Escherichia coli
1.1.1.4 NADPH NADH is preferred over NADPH Escherichia coli
1.1.1.304 NADH
-
 Klebsiella pneumoniae

General Information

EC Number General Information Comment Organism
1.1.1.4 physiological function glycerol/1,2-propanediol dehydrogenase GldA is the major enzyme responsible for the acetoin reducing activity observed in Escherichia coli Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.4 0.04
-
 acetoin cosubstrate NADPH, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.4 0.4
-
 acetoin cosubstrate NADH, pH 7.0, temperature not specified in the publication Escherichia coli