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Literature summary extracted from

  • Liang, K.; Shen, C.R.
    Selection of an endogenous 2,3-butanediol pathway in Escherichia coli by fermentative redox balance (2017), Metab. Eng., 39, 181-191.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.4 3.4
-
 acetoin cosubstrate NADH, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.4 13.6
-
 acetoin cosubstrate NADPH, pH 7.0, temperature not specified in the publication Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.4 Escherichia coli
-
-
-
1.1.1.4 Escherichia coli BW25113
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.4 acetoin + NADH + H+
-
 Escherichia coli (R,R)-butanediol + NAD+
-
 ?
1.1.1.4 acetoin + NADH + H+
-
 Escherichia coli BW25113 (R,R)-butanediol + NAD+
-
 ?
1.1.1.4 acetoin + NADPH + H+
-
 Escherichia coli (R,R)-butanediol + NADP+
-
 ?
1.1.1.4 acetoin + NADPH + H+
-
 Escherichia coli BW25113 (R,R)-butanediol + NADP+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.4 0.5
-
 acetoin cosubstrate NADPH, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.4 1.4
-
 acetoin cosubstrate NADH, pH 7.0, temperature not specified in the publication Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.4 NADH preferred over NADPH Escherichia coli
1.1.1.4 NADPH NADH is preferred over NADPH Escherichia coli

General Information

EC Number General Information Comment Organism
1.1.1.4 physiological function glycerol/1,2-propanediol dehydrogenase GldA is the major enzyme responsible for the acetoin reducing activity observed in Escherichia coli Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.4 0.04
-
 acetoin cosubstrate NADPH, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.4 0.4
-
 acetoin cosubstrate NADH, pH 7.0, temperature not specified in the publication Escherichia coli