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Literature summary extracted from

  • Menon, B.R.; Hardman, S.J.; Scrutton, N.S.; Heyes, D.J.
    Multiple active site residues are important for photochemical efficiency in the light-activated enzyme protochlorophyllide oxidoreductase (POR) (2016), J. Photochem. Photobiol. B, 161, 236-243.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.33 expressed in Escherichia coli BL21(DE3) cells Thermosynechococcus vestitus

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.33 G19A the mutant shows wild type value for turnover number Thermosynechococcus vestitus
1.3.1.33 H236A the mutant shows reduced value for turnover number compared to the wild type enzyme Thermosynechococcus vestitus
1.3.1.33 K42A the mutant shows wild type value for turnover number Thermosynechococcus vestitus
1.3.1.33 N149V the mutant shows reduced value for turnover number compared to the wild type enzyme Thermosynechococcus vestitus
1.3.1.33 N39V the mutant shows reduced value for turnover number compared to the wild type enzyme Thermosynechococcus vestitus
1.3.1.33 N90A the mutant shows reduced value for turnover number compared to the wild type enzyme Thermosynechococcus vestitus
1.3.1.33 R38V the mutant shows wild type value for turnover number Thermosynechococcus vestitus
1.3.1.33 S16C the mutant shows wild type value for turnover number Thermosynechococcus vestitus
1.3.1.33 S189A the mutant shows wild type value for turnover number Thermosynechococcus vestitus
1.3.1.33 T145A the mutant shows reduced value for turnover number compared to the wild type enzyme Thermosynechococcus vestitus
1.3.1.33 T147F the mutant shows reduced value for turnover number compared to the wild type enzyme Thermosynechococcus vestitus
1.3.1.33 T147S the mutant shows reduced value for turnover number compared to the wild type enzyme Thermosynechococcus vestitus
1.3.1.33 T230A the mutant shows wild type value for turnover number Thermosynechococcus vestitus
1.3.1.33 T230F the mutant shows reduced value for turnover number compared to the wild type enzyme Thermosynechococcus vestitus
1.3.1.33 T230S the mutant shows wild type value for turnover number Thermosynechococcus vestitus
1.3.1.33 Y94F the mutant shows wild type value for turnover number Thermosynechococcus vestitus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.33 protochlorophyllide + NADPH + H+ Thermosynechococcus vestitus
-
 chlorophyllide a + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.33 Thermosynechococcus vestitus Q8DLC1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.33 protochlorophyllide + NADPH + H+
-
 Thermosynechococcus vestitus chlorophyllide a + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.33 light-activated enzyme protochlorophyllide oxidoreductase
-
 Thermosynechococcus vestitus
1.3.1.33 POR
-
 Thermosynechococcus vestitus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.33 0.01
-
 protochlorophyllide mutant enzyme N149V, at 25°C, pH not specified in the publication Thermosynechococcus vestitus
1.3.1.33 0.01
-
 protochlorophyllide mutant enzyme T145A, at 25°C, pH not specified in the publication Thermosynechococcus vestitus
1.3.1.33 0.01
-
 protochlorophyllide mutant enzyme T147F, at 25°C, pH not specified in the publication Thermosynechococcus vestitus
1.3.1.33 0.01
-
 protochlorophyllide mutant enzyme T230F, at 25°C, pH not specified in the publication Thermosynechococcus vestitus
1.3.1.33 0.02
-
 protochlorophyllide mutant enzyme N39V, at 25°C, pH not specified in the publication Thermosynechococcus vestitus
1.3.1.33 0.02
-
 protochlorophyllide mutant enzyme T147S, at 25°C, pH not specified in the publication Thermosynechococcus vestitus
1.3.1.33 0.07
-
 protochlorophyllide mutant enzyme N90A, at 25°C, pH not specified in the publication Thermosynechococcus vestitus
1.3.1.33 0.1
-
 protochlorophyllide mutant enzyme H236A, at 25°C, pH not specified in the publication Thermosynechococcus vestitus
1.3.1.33 0.16
-
 protochlorophyllide mutant enzyme G19A, at 25°C, pH not specified in the publication Thermosynechococcus vestitus
1.3.1.33 0.16
-
 protochlorophyllide mutant enzyme K42A, at 25°C, pH not specified in the publication Thermosynechococcus vestitus
1.3.1.33 0.16
-
 protochlorophyllide mutant enzyme R38V, at 25°C, pH not specified in the publication Thermosynechococcus vestitus
1.3.1.33 0.16
-
 protochlorophyllide mutant enzyme S16C, at 25°C, pH not specified in the publication Thermosynechococcus vestitus
1.3.1.33 0.16
-
 protochlorophyllide mutant enzyme S189A, at 25°C, pH not specified in the publication Thermosynechococcus vestitus
1.3.1.33 0.16
-
 protochlorophyllide mutant enzyme T230A, at 25°C, pH not specified in the publication Thermosynechococcus vestitus
1.3.1.33 0.16
-
 protochlorophyllide mutant enzyme T230S, at 25°C, pH not specified in the publication Thermosynechococcus vestitus
1.3.1.33 0.17
-
 protochlorophyllide wild type enzyme, at 25°C, pH not specified in the publication Thermosynechococcus vestitus
1.3.1.33 0.17
-
 protochlorophyllide mutant enzyme Y94F, at 25°C, pH not specified in the publication Thermosynechococcus vestitus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.33 NADPH
-
 Thermosynechococcus vestitus