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Literature summary extracted from

  • Zhou, Y.; Zhou, S.; Yu, H.; Li, J.; Xia, Y.; Li, B.; Wang, X.; Wang, P.
    Cloning and characterization of filamentous fungal S-nitrosoglutathione reductase from Aspergillus nidulans (2016), J. Microbiol. Biotechnol., 26, 928-937.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.284 C100A mutant is misfolded Aspergillus nidulans
1.1.1.284 C11A about 55% of wild-type activity Aspergillus nidulans
1.1.1.284 C48A about 5% of wild-type activity Aspergillus nidulans
1.1.1.284 R118A about 75% of wild-type activity Aspergillus nidulans
1.1.1.284 T50A about 85% of wild-type activity Aspergillus nidulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.284 0.55
-
 S-nitrosoglutathione pH 6.0, 37°C Aspergillus nidulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.284 40000
-
 2 * 40000, SDS-PAGE Aspergillus nidulans
1.1.1.284 75000
-
 gel filtration Aspergillus nidulans

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.284 Aspergillus nidulans Q5AVP8
-
-
1.1.1.284 Aspergillus nidulans ATCC 38163 Q5AVP8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.284 additional information enzyme additionally has aldehyde reductase activity, acting on formaldehyde, acetaldehyde, propionaldehyde with 2-20% of the activity with S-nitrosoglutathione Aspergillus nidulans ?
-
 ?
1.1.1.284 additional information enzyme additionally has aldehyde reductase activity, acting on formaldehyde, acetaldehyde, propionaldehyde with 2-20% of the activity with S-nitrosoglutathione Aspergillus nidulans ATCC 38163 ?
-
 ?
1.1.1.284 S-nitrosoglutathione + NADH + H+
-
 Aspergillus nidulans glutathione disulfide + hydroxylamine + NH4+ + NAD+
-
 ?
1.1.1.284 S-nitrosoglutathione + NADH + H+
-
 Aspergillus nidulans ATCC 38163 glutathione disulfide + hydroxylamine + NH4+ + NAD+
-
 ?
1.1.1.284 S-nitrosoglutathione + NADPH + H+
-
 Aspergillus nidulans glutathione disulfide + hydroxylamine + NH4+ + NADP+
-
 ?
1.1.1.284 S-nitrosoglutathione + NADPH + H+
-
 Aspergillus nidulans ATCC 38163 glutathione disulfide + hydroxylamine + NH4+ + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.284 dimer 2 * 40000, SDS-PAGE Aspergillus nidulans

Synonyms

EC Number Synonyms Comment Organism
1.1.1.284 AN7632.2
-
 Aspergillus nidulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.284 37
-
-
 Aspergillus nidulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.284 45
-
 4 min, 60% residual activity Aspergillus nidulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.284 6
-
-
 Aspergillus nidulans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.284 NADH preferred over NADPH Aspergillus nidulans
1.1.1.284 NADPH 55% of the activity with NADH Aspergillus nidulans