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Literature summary extracted from

  • Bertoletti, N.; Braun, F.; Lepage, M.; Moeller, G.; Adamski, J.; Heine, A.; Klebe, G.; Marchais-Oberwinkler, S.
    New insights into human 17beta-hydroxysteroid dehydrogenase type 14: first crystal structures in complex with a steroidal ligand and with a potent nonsteroidal inhibitor (2016), J. Med. Chem., 59, 6961-6967.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.62 recombinant expression of 17beta-HSD14 wild-type variants S205 and T205 in Escherichia coli strain BL21 pLysS Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.62 the purified enzyme variants T205 and S205 in apoform, as holoenzyme, or in complex with estrone or inhibitor [6-(3,4-dihydroxyphenyl)pyridin-2-yl]-(4-fluoro-3-hydroxyphenyl)methanone, ternary complex enzyme-cofactor-inhibitor, X-ray diffraction structure determination and analysis at 1.52-2.02 A resolution Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.62 S205T natural genetic polymorphism Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.62 [6-(3,4-dihydroxyphenyl)pyridin-2-yl]-(4-fluoro-3-hydroxyphenyl)methanone a potent nonsteroidal inhibitor, binding structure, overview Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.62 additional information
-
 additional information Michaelis-Menten kinetics Homo sapiens
1.1.1.62 0.00062
-
 17beta-estradiol 17beta-HSD14 variant S205, pH and temperature not specified in the publication Homo sapiens
1.1.1.62 0.00079
-
 17beta-estradiol 17beta-HSD14 variant T205, pH and temperature not specified in the publication Homo sapiens
1.1.1.62 0.0066
-
 5-androstene-3beta,17beta-diol 17beta-HSD14 variant S205, pH and temperature not specified in the publication Homo sapiens
1.1.1.62 0.0078
-
 5-androstene-3beta,17beta-diol 17beta-HSD14 variant T205, pH and temperature not specified in the publication Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.62 cytosol
-
 Homo sapiens 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.62 17beta-estradiol + NAD+ Homo sapiens
-
 estrone + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.62 Homo sapiens Q9BPX1
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.62 adrenal gland
-
 Homo sapiens
-
1.1.1.62 brain
-
 Homo sapiens
-
1.1.1.62 breast cancer cell
-
 Homo sapiens
-
1.1.1.62 esophagus
-
 Homo sapiens
-
1.1.1.62 eye
-
 Homo sapiens
-
1.1.1.62 heart
-
 Homo sapiens
-
1.1.1.62 kidney
-
 Homo sapiens
-
1.1.1.62 liver
-
 Homo sapiens
-
1.1.1.62 placenta
-
 Homo sapiens
-
1.1.1.62 rectum
-
 Homo sapiens
-
1.1.1.62 salivary gland
-
 Homo sapiens
-
1.1.1.62 skeletal muscle
-
 Homo sapiens
-
1.1.1.62 testis
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.62 17beta-estradiol + NAD+
-
 Homo sapiens estrone + NADH + H+
-
 r
1.1.1.62 5-androstene-3beta,17beta-diol + NAD+
-
 Homo sapiens ? + NADH + H+
-
 ?
1.1.1.62 testosterone + NAD+
-
 Homo sapiens androst-4-ene-3,17-dione + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.62 17beta-HSD14
-
 Homo sapiens
1.1.1.62 17beta-hydroxysteroid dehydrogenase type 14
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.62 0.00028
-
 5-androstene-3beta,17beta-diol 17beta-HSD14 variant S205, pH and temperature not specified in the publication Homo sapiens
1.1.1.62 0.00032
-
 5-androstene-3beta,17beta-diol 17beta-HSD14 variant T205, pH and temperature not specified in the publication Homo sapiens
1.1.1.62 0.0004
-
 17beta-estradiol 17beta-HSD14 variant S205, pH and temperature not specified in the publication Homo sapiens
1.1.1.62 0.00055
-
 17beta-estradiol 17beta-HSD14 variant T205, pH and temperature not specified in the publication Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.62 NAD+
-
 Homo sapiens
1.1.1.62 NADH
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.62 0.000007
-
 [6-(3,4-dihydroxyphenyl)pyridin-2-yl]-(4-fluoro-3-hydroxyphenyl)methanone pH and temperature not specified in the publication Homo sapiens