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Literature summary extracted from

  • Camadan, Y.; Oezdemir, H.; Gulcin, I.
    Purification and characterization of dihydropyrimidine dehydrogenase enzyme from sheep liver and determination of the effects of some anaesthetic and antidepressant drugs on the enzyme activity (2016), J. Enzyme Inhib. Med. Chem., 31, 1335-1341.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.3.1.2 maximum enzyme activity is obtained in 0.28 M phosphate buffer (pH 8.0) Ovis aries

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.2 escitalopram
-
 Ovis aries
1.3.1.2 fluoxetine
-
 Ovis aries
1.3.1.2 haloperidol
-
 Ovis aries
1.3.1.2 lidocaine
-
 Ovis aries
1.3.1.2 mirtazapine
-
 Ovis aries
1.3.1.2 propofol
-
 Ovis aries

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.2 0.01746
-
 Uracil at pH 7.4 and 25°C Ovis aries
1.3.1.2 0.02297
-
 NADPH at pH 7.4 and 25°C Ovis aries

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.2 111000
-
 gel filtration Ovis aries

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.2 uracil + NADPH + H+ Ovis aries
-
 5,6-dihydrouracil + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.2 Ovis aries
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.2 ammonium sulfate precipitation, DEAE-cellulose column chromatography, and 2',5'-ADP Sepharose-4B column chromatography Ovis aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.2 liver
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.2 0.00173
-
 crude enzyme, at pH 7.4 and 25°C Ovis aries
1.3.1.2 0.705
-
 after 407.5fold purification, at pH 7.4 and 25°C Ovis aries

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.2 uracil + NADPH + H+
-
 Ovis aries 5,6-dihydrouracil + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.2 DPD
-
 Ovis aries

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.2 50
-
-
 Ovis aries

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.2 8
-
-
 Ovis aries

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.3.1.2 6
-
 the enzyme remains stable after 12 h at pH 6.0 Ovis aries

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.2 NADPH
-
 Ovis aries

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.3.1.2 0.00021
-
 at pH 7.4 and 25°C Ovis aries propofol
1.3.1.2 0.01324
-
 at pH 7.4 and 25°C Ovis aries fluoxetine
1.3.1.2 0.01507
-
 at pH 7.4 and 25°C Ovis aries lidocaine
1.3.1.2 0.08665
-
 at pH 7.4 and 25°C Ovis aries mirtazapine
1.3.1.2 0.09903
-
 at pH 7.4 and 25°C Ovis aries haloperidol
1.3.1.2 1.736
-
 at pH 7.4 and 25°C Ovis aries escitalopram