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Literature summary extracted from

  • Eggert, T.; Bakonyi, D.; Hummel, W.
    Enzymatic routes for the synthesis of ursodeoxycholic acid (2014), J. Biotechnol., 191, 11-21.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.159 synthesis the enzyme is useful in production of ursodeoxycholic acid, a secondary bile acid, which is used as a drug for the treatment of various liver diseases Escherichia coli
1.1.1.159 synthesis the enzyme is useful in production of ursodeoxycholic acid, a secondary bile acid, which is used as a drug for the treatment of various liver diseases Pseudomonas sp.
1.1.1.159 synthesis the enzyme is useful in production of ursodeoxycholic acid, a secondary bile acid, which is used as a drug for the treatment of various liver diseases Bacteroides fragilis
1.1.1.159 synthesis the enzyme is useful in production of ursodeoxycholic acid, a secondary bile acid, which is used as a drug for the treatment of various liver diseases Stenotrophomonas maltophilia
1.1.1.176 synthesis the enzyme is useful in production of ursodeoxycholic acid, a secondary bile acid, which is used as a drug for the treatment of various liver diseases Clostridium sp.
1.1.1.176 synthesis the enzyme is useful in production of ursodeoxycholic acid, a secondary bile acid, which is used as a drug for the treatment of various liver diseases Clostridium perfringens
1.1.1.176 synthesis the enzyme is useful in production of ursodeoxycholic acid, a secondary bile acid, which is used as a drug for the treatment of various liver diseases Eggerthella lenta
1.1.1.176 synthesis the enzyme is useful in production of ursodeoxycholic acid, a secondary bile acid, which is used as a drug for the treatment of various liver diseases [Clostridium] leptum
1.1.1.201 synthesis the enzyme is useful in production of ursodeoxycholic acid, a secondary bile acid, which is used as a drug for the treatment of various liver diseases Clostridium sardiniense
1.1.1.201 synthesis the enzyme is useful in production of ursodeoxycholic acid, a secondary bile acid, which is used as a drug for the treatment of various liver diseases Paeniclostridium sordellii
1.1.1.201 synthesis the enzyme is useful in production of ursodeoxycholic acid, a secondary bile acid, which is used as a drug for the treatment of various liver diseases Fusarium equiseti
1.1.1.201 synthesis the enzyme is useful in production of ursodeoxycholic acid, a secondary bile acid, which is used as a drug for the treatment of various liver diseases [Clostridium] scindens
1.1.1.201 synthesis the enzyme is useful in production of ursodeoxycholic acid, a secondary bile acid, which is used as a drug for the treatment of various liver diseases Collinsella aerofaciens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.159 CA 7alpha-HSDH, recombinant overexpression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Clostridium sardiniense

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.159 purified recombinant enzyme complexed with taurochenodeoxycholic acid and NADP+, sitting drop vapour diffusion method, mixing of 200 nl of 1.12 mM protein in 50 mM Tris-HCl, pH 8.0, 200 mM NaCl,and 5.6 mM of NADP+ and taurochenodeoxycholic acid, with 200 nl of reservoir solution containing 0.1 M HEPES, pH 7.5, and 25% PEG-3350, and equilibration against 0.03 ml reservoir solution, at 20°C, X-ray diffraction structure determination and analysis at 2.0 A resolution Clostridium sardiniense

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.159 additional information development and evaluation of a selected multi-step reaction system for the synthesis of ursodeoxycholic acid, separation of each step by isolation of the intermediates using ultrafiltration membranes, overview Escherichia coli
1.1.1.159 additional information development and evaluation of a selected multi-step reaction system for the synthesis of ursodeoxycholic acid, separation of each step by isolation of the intermediates using ultrafiltration membranes, overview Pseudomonas sp.
1.1.1.159 additional information development and evaluation of a selected multi-step reaction system for the synthesis of ursodeoxycholic acid, separation of each step by isolation of the intermediates using ultrafiltration membranes, overview Bacteroides fragilis
1.1.1.159 additional information development and evaluation of a selected multi-step reaction system for the synthesis of ursodeoxycholic acid, separation of each step by isolation of the intermediates using ultrafiltration membranes, overview Stenotrophomonas maltophilia
1.1.1.159 R16G site-directed mutagenesis, kcat and Km of the R16G mutant increase by more than 4times and 5times compared with wild-type values, respectively, while the catalytic efficiency (kcat/Km) of R16G mutant decreases by 17.26% compared to the wild-type enzyme. The increase in Km indicates that affinity of R16G mutant toward NADP+ becomes weak, while the cofactor NADP(H) dissociates more easily from the binding site resulting in the increase in kcat Clostridium sardiniense
1.1.1.159 R194G site-directed mutagenesis, the mutant shows slightly reduced catalytic efficiency compared with NADP+ compared to the wild-type enzyme Clostridium sardiniense
1.1.1.176 additional information development and evaluation of a selected multi-step reaction system for the synthesis of ursodeoxycholic acid, separation of each step by isolation of the intermediates using ultrafiltration membranes, overview Clostridium perfringens
1.1.1.176 additional information development and evaluation of a selected multi-step reaction system for the synthesis of ursodeoxycholic acid, separation of each step by isolation of the intermediates using ultrafiltration membranes, overview Eggerthella lenta
1.1.1.176 additional information development and evaluation of a selected multi-step reaction system for the synthesis of ursodeoxycholic acid, separation of each step by isolation of the intermediates using ultrafiltration membranes, overview [Clostridium] leptum
1.1.1.176 additional information development and evaluation of a selected multi-step reaction system for the synthesis of ursodeoxycholic acid. Multistep conversion of cholic acid into ursodeoxycholic acid. Enzyme sources are 7alpha-HSDH from Clostridium absonum (crude extract), 12alpha-HSDH from Clostridium group P, 7beta-HSDH from Clostridium absonum, method, separation of each step by isolation of the intermediates using ultrafiltration membranes, overview Clostridium sp.
1.1.1.201 additional information development and evaluation of a selected multi-step reaction system for the synthesis of ursodeoxycholic acid, separation of each step by isolation of the intermediates using ultrafiltration membranes, overview Clostridium sardiniense
1.1.1.201 additional information development and evaluation of a selected multi-step reaction system for the synthesis of ursodeoxycholic acid, separation of each step by isolation of the intermediates using ultrafiltration membranes, overview Paeniclostridium sordellii
1.1.1.201 additional information development and evaluation of a selected multi-step reaction system for the synthesis of ursodeoxycholic acid, separation of each step by isolation of the intermediates using ultrafiltration membranes, overview Fusarium equiseti
1.1.1.201 additional information development and evaluation of a selected multi-step reaction system for the synthesis of ursodeoxycholic acid, separation of each step by isolation of the intermediates using ultrafiltration membranes, overview [Clostridium] scindens
1.1.1.201 additional information development and evaluation of a selected multi-step reaction system for the synthesis of ursodeoxycholic acid, separation of each step by isolation of the intermediates using ultrafiltration membranes, overview Collinsella aerofaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.159 0.34
-
 NADP+ wild-type enzyme, pH and temperature not specified in the publication Clostridium sardiniense
1.1.1.159 0.72
-
 NADP+ mutant R194G, pH and temperature not specified in the publication Clostridium sardiniense
1.1.1.159 1.77
-
 NADP+ mutant R16G, pH and temperature not specified in the publication Clostridium sardiniense

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.159 cholate + NAD+ Escherichia coli
-
 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H+
-
 r
1.1.1.159 cholate + NAD+ Pseudomonas sp.
-
 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H+
-
 r
1.1.1.159 cholate + NAD+ Acinetobacter calcoaceticus
-
 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H+
-
 r
1.1.1.159 cholate + NAD+ Bacteroides fragilis
-
 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H+
-
 r
1.1.1.159 cholate + NAD+ Stenotrophomonas maltophilia
-
 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H+
-
 r
1.1.1.176 cholate + NAD+ Clostridium perfringens
-
 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADH + H+
-
 ?
1.1.1.176 cholate + NAD+ Eggerthella lenta
-
 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADH + H+
-
 ?
1.1.1.176 cholate + NADP+ Clostridium sp.
-
 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
 ?
1.1.1.176 cholate + NADP+ [Clostridium] leptum
-
 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
 ?
1.1.1.176 cholate + NADP+ Clostridium sp. ATCC 29733
-
 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
 ?
1.1.1.176 additional information Clostridium sp. multistep conversion of cholic acid into ursodeoxycholic acid. Enzyme sources are 7alpha-HSDH from Clostridium absonum (crude extract), 12alpha-HSDH from Clostridium group P, 7beta-HSDH from Clostridium absonum ?
-
 ?
1.1.1.176 additional information Clostridium sp. ATCC 29733 multistep conversion of cholic acid into ursodeoxycholic acid. Enzyme sources are 7alpha-HSDH from Clostridium absonum (crude extract), 12alpha-HSDH from Clostridium group P, 7beta-HSDH from Clostridium absonum ?
-
 ?
1.1.1.201 chenodeoxycholic acid + NADPH + H+ Clostridium sardiniense
-
 ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 chenodeoxycholic acid + NADPH + H+ Paeniclostridium sordellii
-
 ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 chenodeoxycholic acid + NADPH + H+ Fusarium equiseti
-
 ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 chenodeoxycholic acid + NADPH + H+ [Clostridium] scindens
-
 ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 chenodeoxycholic acid + NADPH + H+ Collinsella aerofaciens
-
 ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 lithocholic acid + NADPH + H+ Paeniclostridium sordellii
-
 ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 lithocholic acid + NADPH + H+ Fusarium equiseti
-
 ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 lithocholic acid + NADPH + H+ [Clostridium] scindens
-
 ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 lithocholic acid + NADPH + H+ Collinsella aerofaciens
-
 ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 lithocholic acid + NADPH + H+ Clostridium sardiniense presence of both 7alpha-HSDH (EC 1.1.1.159) and 7beta-HSDH in one organism allows epimerization by a single bacterium ursodeoxycholic acid + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.159 Acinetobacter calcoaceticus
-
-
-
1.1.1.159 Bacteroides fragilis
-
-
-
1.1.1.159 Clostridium sardiniense G9FRD7
-
-
1.1.1.159 Escherichia coli
-
-
-
1.1.1.159 Pseudomonas sp.
-
-
-
1.1.1.159 Stenotrophomonas maltophilia
-
-
-
1.1.1.176 Clostridium perfringens
-
-
-
1.1.1.176 Clostridium sp.
-
 group P C 48-50
-
1.1.1.176 Clostridium sp. ATCC 29733
-
 group P C 48-50
-
1.1.1.176 Eggerthella lenta
-
 formerly Eubacterium lentum
-
1.1.1.176 [Clostridium] leptum
-
-
-
1.1.1.201 Clostridium sardiniense
-
-
-
1.1.1.201 Collinsella aerofaciens
-
-
-
1.1.1.201 Fusarium equiseti
-
-
-
1.1.1.201 Paeniclostridium sordellii
-
-
-
1.1.1.201 [Clostridium] scindens
-
 formerly Eubacterium sp.
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.159 recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, anion exchange chromatography, and gel filtration Clostridium sardiniense

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.159 70
-
 pH and temperature not specified in the publication Acinetobacter calcoaceticus
1.1.1.159 70
-
 pH and temperature not specified in the publication Stenotrophomonas maltophilia
1.1.1.159 70
-
 pH and temperature not specified in the publication Clostridium sardiniense
1.1.1.159 900
-
 pH and temperature not specified in the publication Pseudomonas sp.
1.1.1.201 350
-
 pH and temperature not specified in the publication Paeniclostridium sordellii
1.1.1.201 350
-
 pH and temperature not specified in the publication [Clostridium] scindens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.159 chenodeoxycholic acid + NAD+
-
 Stenotrophomonas maltophilia 7-oxolithocholic acid + NADH + H+
-
 ?
1.1.1.159 cholate + NAD+
-
 Escherichia coli 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H+
-
 r
1.1.1.159 cholate + NAD+
-
 Pseudomonas sp. 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H+
-
 r
1.1.1.159 cholate + NAD+
-
 Acinetobacter calcoaceticus 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H+
-
 r
1.1.1.159 cholate + NAD+
-
 Bacteroides fragilis 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H+
-
 r
1.1.1.159 cholate + NAD+
-
 Stenotrophomonas maltophilia 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H+
-
 r
1.1.1.159 lithocholic acid + NADPH + H+ presence of both 7alpha-HSDH and 7beta-HSDH (EC 1.1.1.201) in one organism allows epimerization by a single bacterium Escherichia coli ursodeoxycholic acid + NADP+
-
 ?
1.1.1.159 lithocholic acid + NADPH + H+ presence of both 7alpha-HSDH and 7beta-HSDH (EC 1.1.1.201) in one organism allows epimerization by a single bacterium Pseudomonas sp. ursodeoxycholic acid + NADP+
-
 ?
1.1.1.159 lithocholic acid + NADPH + H+ presence of both 7alpha-HSDH and 7beta-HSDH (EC 1.1.1.201) in one organism allows epimerization by a single bacterium Bacteroides fragilis ursodeoxycholic acid + NADP+
-
 ?
1.1.1.159 lithocholic acid + NADPH + H+ presence of both 7alpha-HSDH and 7beta-HSDH (EC 1.1.1.201) in one organism allows epimerization by a single bacterium Stenotrophomonas maltophilia ursodeoxycholic acid + NADP+
-
 ?
1.1.1.159 taurochenodeoxycholic acid + NADP+
-
 Clostridium sardiniense ? + NADPH + H+
-
 r
1.1.1.159 ursodeoxycholic acid + NAD+
-
 Stenotrophomonas maltophilia 7-oxolithocholic acid + NADH + H+
-
 ?
1.1.1.176 cholate + NAD+
-
 Clostridium perfringens 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADH + H+
-
 ?
1.1.1.176 cholate + NAD+
-
 Eggerthella lenta 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADH + H+
-
 ?
1.1.1.176 cholate + NADP+
-
 Clostridium sp. 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
 ?
1.1.1.176 cholate + NADP+
-
 [Clostridium] leptum 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
 ?
1.1.1.176 cholate + NADP+ conversion of CA into 12-oxoursodeoxycholic acid Clostridium sp. 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
 ?
1.1.1.176 cholate + NADP+ conversion of CA into 12-oxoursodeoxycholic acid Clostridium perfringens 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
 ?
1.1.1.176 cholate + NADP+ conversion of CA into 12-oxoursodeoxycholic acid Eggerthella lenta 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
 ?
1.1.1.176 cholate + NADP+ conversion of CA into 12-oxoursodeoxycholic acid [Clostridium] leptum 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
 ?
1.1.1.176 cholate + NADP+
-
 Clostridium sp. ATCC 29733 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
 ?
1.1.1.176 cholate + NADP+ conversion of CA into 12-oxoursodeoxycholic acid Clostridium sp. ATCC 29733 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
 ?
1.1.1.176 additional information multistep conversion of cholic acid into ursodeoxycholic acid. Enzyme sources are 7alpha-HSDH from Clostridium absonum (crude extract), 12alpha-HSDH from Clostridium group P, 7beta-HSDH from Clostridium absonum Clostridium sp. ?
-
 ?
1.1.1.176 additional information multistep conversion of cholic acid into ursodeoxycholic acid. Enzyme sources are 7alpha-HSDH from Clostridium absonum (crude extract), 12alpha-HSDH from Clostridium group P, 7beta-HSDH from Clostridium absonum Clostridium sp. ATCC 29733 ?
-
 ?
1.1.1.201 7,12-dioxo-ursodeoxycholic acid + NADPH + H+
-
 Clostridium sardiniense 12-oxo-ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 7,12-dioxo-ursodeoxycholic acid + NADPH + H+
-
 Paeniclostridium sordellii 12-oxo-ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 7,12-dioxo-ursodeoxycholic acid + NADPH + H+
-
 Fusarium equiseti 12-oxo-ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 7,12-dioxo-ursodeoxycholic acid + NADPH + H+
-
 [Clostridium] scindens 12-oxo-ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 7,12-dioxo-ursodeoxycholic acid + NADPH + H+
-
 Collinsella aerofaciens 12-oxo-ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 chenodeoxycholic acid + NADPH + H+
-
 Clostridium sardiniense ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 chenodeoxycholic acid + NADPH + H+
-
 Paeniclostridium sordellii ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 chenodeoxycholic acid + NADPH + H+
-
 Fusarium equiseti ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 chenodeoxycholic acid + NADPH + H+
-
 [Clostridium] scindens ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 chenodeoxycholic acid + NADPH + H+
-
 Collinsella aerofaciens ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 dehydrocholic acid + NADPH + H+
-
 Clostridium sardiniense 12-oxo-ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 dehydrocholic acid + NADPH + H+
-
 Paeniclostridium sordellii 12-oxo-ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 dehydrocholic acid + NADPH + H+
-
 Fusarium equiseti 12-oxo-ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 dehydrocholic acid + NADPH + H+
-
 [Clostridium] scindens 12-oxo-ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 dehydrocholic acid + NADPH + H+
-
 Collinsella aerofaciens 12-oxo-ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 lithocholic acid + NADPH + H+
-
 Clostridium sardiniense ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 lithocholic acid + NADPH + H+
-
 Paeniclostridium sordellii ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 lithocholic acid + NADPH + H+
-
 Fusarium equiseti ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 lithocholic acid + NADPH + H+
-
 [Clostridium] scindens ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 lithocholic acid + NADPH + H+
-
 Collinsella aerofaciens ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 lithocholic acid + NADPH + H+ presence of both 7alpha-HSDH (EC 1.1.1.159) and 7beta-HSDH in one organism allows epimerization by a single bacterium Clostridium sardiniense ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 lithocholic acid + NADPH + H+ presence of both 7alpha-HSDH (EC 1.1.1.159) and 7beta-HSDH in one organism allows epimerization by a single bacterium Paeniclostridium sordellii ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 lithocholic acid + NADPH + H+ presence of both 7alpha-HSDH (EC 1.1.1.159) and 7beta-HSDH in one organism allows epimerization by a single bacterium Fusarium equiseti ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 lithocholic acid + NADPH + H+ presence of both 7alpha-HSDH (EC 1.1.1.159) and 7beta-HSDH in one organism allows epimerization by a single bacterium [Clostridium] scindens ursodeoxycholic acid + NADP+
-
 ?
1.1.1.201 lithocholic acid + NADPH + H+ presence of both 7alpha-HSDH (EC 1.1.1.159) and 7beta-HSDH in one organism allows epimerization by a single bacterium Collinsella aerofaciens ursodeoxycholic acid + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.159 tetramer the enzyme possesses the typical alpha/beta folding pattern. The residues Glu258, Gln255 and Thr253 in one subunit form hydrogen bonds with the residues His211/Ser207, Ser207, Pro151 in the other subunit, respectively Clostridium sardiniense

Synonyms

EC Number Synonyms Comment Organism
1.1.1.159 7alpha-HSDH
-
 Clostridium sardiniense
1.1.1.159 7alpha-hydroxysteroid dehydrogenase
-
 Escherichia coli
1.1.1.159 7alpha-hydroxysteroid dehydrogenase
-
 Pseudomonas sp.
1.1.1.159 7alpha-hydroxysteroid dehydrogenase
-
 Acinetobacter calcoaceticus
1.1.1.159 7alpha-hydroxysteroid dehydrogenase
-
 Bacteroides fragilis
1.1.1.159 7alpha-hydroxysteroid dehydrogenase
-
 Stenotrophomonas maltophilia
1.1.1.159 CA 7alpha-HSDH
-
 Clostridium sardiniense
1.1.1.176 12alpha-HSDH
-
 Clostridium sp.
1.1.1.176 12alpha-HSDH
-
 Clostridium perfringens
1.1.1.176 12alpha-HSDH
-
 Eggerthella lenta
1.1.1.176 12alpha-HSDH
-
 [Clostridium] leptum
1.1.1.176 NAD+-dependent 12alpha-HSDH
-
 Clostridium perfringens
1.1.1.176 NAD+-dependent 12alpha-HSDH
-
 Eggerthella lenta
1.1.1.176 NADP+-dependent 12alpha-HSDH
-
 Clostridium sp.
1.1.1.176 NADP+-dependent 12alpha-HSDH
-
 [Clostridium] leptum
1.1.1.201 7beta-HSDH
-
 Clostridium sardiniense
1.1.1.201 7beta-HSDH
-
 Paeniclostridium sordellii
1.1.1.201 7beta-HSDH
-
 Fusarium equiseti
1.1.1.201 7beta-HSDH
-
 [Clostridium] scindens
1.1.1.201 7beta-HSDH
-
 Collinsella aerofaciens
1.1.1.201 7beta-hydroxysteroid dehydrogenase
-
 Clostridium sardiniense
1.1.1.201 7beta-hydroxysteroid dehydrogenase
-
 Paeniclostridium sordellii
1.1.1.201 7beta-hydroxysteroid dehydrogenase
-
 Fusarium equiseti
1.1.1.201 7beta-hydroxysteroid dehydrogenase
-
 [Clostridium] scindens
1.1.1.201 7beta-hydroxysteroid dehydrogenase
-
 Collinsella aerofaciens
1.1.1.201 RUMGNA_02585
-
 Fusarium equiseti

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.159 25
-
 completely stable for 108 h Clostridium sardiniense

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.159 13.76
-
 NADP+ wild-type enzyme, pH and temperature not specified in the publication Clostridium sardiniense
1.1.1.159 28.66
-
 NADP+ mutant R194G, pH and temperature not specified in the publication Clostridium sardiniense
1.1.1.159 58.55
-
 NADP+ mutant R16G, pH and temperature not specified in the publication Clostridium sardiniense

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.159 NAD+ NAD+-dependent Escherichia coli
1.1.1.159 NAD+ NAD+-dependent Pseudomonas sp.
1.1.1.159 NAD+ NAD+-dependent Acinetobacter calcoaceticus
1.1.1.159 NAD+ NAD+-dependent Bacteroides fragilis
1.1.1.159 NAD+ NAD+-dependent Stenotrophomonas maltophilia
1.1.1.159 NADP+ cofactor binding site structure and active state structure of the NADP+ bound to the enzyme and enzyme mutants, overview. Residue Arg38 can form the stable cation-Pi interaction with the adenine ring of NADP+, and the cation-Pi interaction and hydrogen bonds between Arg38 and NADP+ have a significant anchor effect on the cofactor binding to CA 7alpha-HSDH. Residues Arg16, Arg38 and Arg194 mediate the cofactor specificity and recognition in right orientation Clostridium sardiniense
1.1.1.159 NADPH
-
 Clostridium sardiniense
1.1.1.176 NAD+ NAD+-dependent 12alpha-HSDH Clostridium perfringens
1.1.1.176 NAD+ NAD+-dependent 12alpha-HSDH Eggerthella lenta
1.1.1.176 NADP+ NADP+-dependent 12alpha-HSDH Clostridium sp.
1.1.1.176 NADP+ NADP+-dependent 12alpha-HSDH [Clostridium] leptum
1.1.1.201 NADP+ NADP+-dependent Clostridium sardiniense
1.1.1.201 NADP+ NADP+-dependent Paeniclostridium sordellii
1.1.1.201 NADP+ NADP+-dependent Fusarium equiseti
1.1.1.201 NADP+ NADP+-dependent [Clostridium] scindens
1.1.1.201 NADP+ NADP+-dependent Collinsella aerofaciens

General Information

EC Number General Information Comment Organism
1.1.1.159 evolution 7alpha-HSDHs is a member of SDR superfamily and belongs to the classical subfamily with the cofactor binding site sequence motif TA/G/SxxxGIG and the active site sequence motif YxxxK (x = any amino acid residue) Clostridium sardiniense
1.1.1.159 additional information molecular dynamics simulation of cofactor NADPH and taurochenodeoxycholic acid bound to the enzyme, overview. The C-terminal structure of CA 7alpha-HSDH does not directly surround the substrate-binding pocket but stretch outward. The Ser-Tyr-Lys triad is well conserved in other 7alpha-HSDHs but not in the CA 7alpha-HSDH Clostridium sardiniense
1.1.1.159 physiological function the enzyme CA 7alpha-HSDH is responsible for reversibly catalysing the oxidation of C7alpha-oriented hydroxyl of the steroid nucleus in the bile acid metabolism Clostridium sardiniense

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.159 33.18
-
 NADP+ mutant R16G, pH and temperature not specified in the publication Clostridium sardiniense
1.1.1.159 39.78
-
 NADP+ mutant R194G, pH and temperature not specified in the publication Clostridium sardiniense
1.1.1.159 40.1
-
 NADP+ wild-type enzyme, pH and temperature not specified in the publication Clostridium sardiniense