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Literature summary extracted from

  • Komeda, H.; Yamasaki-Yashiki, S.; Hoshino, K.; Asano, Y.
    Identification and characterization of D-xylose reductase involved in pentose catabolism of the zygomycetous fungus Rhizomucor pusillus (2015), J. Biosci. Bioeng., 119, 57-64.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.431 0.0094
-
 NADPH pH 7.0, 30°C Rhizomucor pusillus
1.1.1.431 0.35
-
 NADH pH 7.0, 30°C Rhizomucor pusillus
1.1.1.431 28.1
-
 D-xylose pH 7.0, 30°C Rhizomucor pusillus
1.1.1.431 36.3
-
 D-ribose pH 7.0, 30°C Rhizomucor pusillus
1.1.1.431 41.7
-
 L-arabinose pH 7.0, 30°C Rhizomucor pusillus
1.1.1.431 72.2
-
 D-galactose pH 7.0, 30°C Rhizomucor pusillus
1.1.1.431 177
-
 D-glucose pH 7.0, 30°C Rhizomucor pusillus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.431 38000
-
-
 Rhizomucor pusillus
1.1.1.431 59000
-
 gel filtration Rhizomucor pusillus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.431 Rhizomucor pusillus I7HD84
-
-
1.1.1.431 Rhizomucor pusillus NBRC 4578 I7HD84
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.431 from mycelium Rhizomucor pusillus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.431 mycelium
-
 Rhizomucor pusillus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.431 D-galactose + NADH + H+ 53% of the activity with D-xylose Rhizomucor pusillus ? + NAD+
-
 ?
1.1.1.431 D-galactose + NADH + H+ 53% of the activity with D-xylose Rhizomucor pusillus NBRC 4578 ? + NAD+
-
 ?
1.1.1.431 D-glucose + NADPH + H+ 38% of the activity with D-xylose Rhizomucor pusillus ? + NADP+
-
 ?
1.1.1.431 D-glucose + NADPH + H+ 38% of the activity with D-xylose Rhizomucor pusillus NBRC 4578 ? + NADP+
-
 ?
1.1.1.431 D-ribose + NADPH + H+ 84% of the activity with D-xylose Rhizomucor pusillus ? + NADP+
-
 ?
1.1.1.431 D-ribose + NADPH + H+ 84% of the activity with D-xylose Rhizomucor pusillus NBRC 4578 ? + NADP+
-
 ?
1.1.1.431 D-xylose + NADPH + H+
-
 Rhizomucor pusillus xylitol + NADP+
-
 ?
1.1.1.431 D-xylose + NADPH + H+
-
 Rhizomucor pusillus NBRC 4578 xylitol + NADP+
-
 ?
1.1.1.431 L-arabinose + NADPH + H+ 79% of the activity with D-xylose Rhizomucor pusillus L-arabinitol + NADP+
-
 ?
1.1.1.431 L-arabinose + NADPH + H+ 79% of the activity with D-xylose Rhizomucor pusillus NBRC 4578 L-arabinitol + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.431 dimer 2 * 36724, calculated, 2 * 38000, SDS-PAGE Rhizomucor pusillus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.431 21.3
-
 D-xylose pH 7.0, 30°C Rhizomucor pusillus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.431 5
-
-
 Rhizomucor pusillus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.431 NADH 2.4% of the activity with NADPH Rhizomucor pusillus
1.1.1.431 NADPH
-
 Rhizomucor pusillus

Expression

EC Number Organism Comment Expression
1.1.1.431 Rhizomucor pusillus transcription of the xyl1 gene is marked in the presence of D-xylose and L-arabinose, but is week in the presence of D-glucose additional information