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Literature summary extracted from

  • Nguyen, N.T.; Yatabe, T.; Yoon, K.S.; Ogo, S.
    Molybdenum-containing membrane-bound formate dehydrogenase isolated from Citrobacter sp. S-77 having high stability against oxygen, pH, and temperature (2014), J. Biosci. Bioeng., 118, 386-391.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.9 0.44
-
 formate at pH 7.0 and 30°C Citrobacter sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.17.1.9 membrane
-
 Citrobacter sp. 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.1.9 Molybdenum contains molybdenum Citrobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.9 32000
-
-
 Citrobacter sp.
1.17.1.9 95000
-
-
 Citrobacter sp.
1.17.1.9 150000
-
 gel filtration Citrobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.9 formate + NAD+ Citrobacter sp.
-
 CO2 + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.9 Citrobacter sp.
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.17.1.9 the oxidized enzyme can be restored up to 80% of its original specific activity, even after the enzyme is exposed to air for 6 h at 4°C Citrobacter sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.9 hydroxyapatite column chromatography, Q Sepharose column chromatography, Resource 15 Q column chromatography, and Superdex 200 gel filtration Citrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.9 formate + benzyl viologen
-
 Citrobacter sp. CO2 + reduced benzyl viologen
-
 ?
1.17.1.9 formate + NAD+
-
 Citrobacter sp. CO2 + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.1.9 ? x * 95000 + x * 32000, SDS-PAGE Citrobacter sp.

Synonyms

EC Number Synonyms Comment Organism
1.17.1.9 FDH
-
 Citrobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.17.1.9 70
-
 the enzyme has a half-life of activity of approximately 20 min at 70°C under anaerobic conditions Citrobacter sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.17.1.9 76
-
 formate at pH 7.0 and 30°C Citrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.1.9 8.5
-
-
 Citrobacter sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.17.1.9 3
-
 the enzyme retains more than 70% of the initial activity after 3 h incubation at pH 3.0 Citrobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.9 molybdenum cofactor
-
 Citrobacter sp.
1.17.1.9 NAD+
-
 Citrobacter sp.
1.17.1.9 [4Fe-4S]-center
-
 Citrobacter sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.17.1.9 173
-
 formate at pH 7.0 and 30°C Citrobacter sp.