EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.25 | gene aroE, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 CodonPlus | Arabidopsis thaliana |
1.1.1.25 | gene aroE, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 CodonPlus | Pseudomonas putida |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.25 | crystal structure analysis, PDB ID 2O7S | Arabidopsis thaliana |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.25 | 2,2-bisepigallocatechin gallate | about 50% inhibition at 0.0025 mM | Pseudomonas putida | |
1.1.1.25 | aurintricarboxylic acid | lower inhibitry potency, about 25% inhibition at 0.0025 mM | Pseudomonas putida | |
1.1.1.25 | baicalein | about 25% inhibition at 0.0025 mM | Pseudomonas putida | |
1.1.1.25 | cardiolipin | lower inhibitry potency, about 25% inhibition at 0.0025 mM | Pseudomonas putida | |
1.1.1.25 | dianthrol | about 50% inhibition at 0.0025 mM | Pseudomonas putida | |
1.1.1.25 | diethylenetriamine pentaacetic acid | lower inhibitry potency, about 25% inhibition at 0.0025 mM | Pseudomonas putida | |
1.1.1.25 | ebselen | lower inhibitry potency, about 25% inhibition at 0.0025 mM | Pseudomonas putida | |
1.1.1.25 | ellagic acid | about 50% inhibition at 0.0025 mM | Pseudomonas putida | |
1.1.1.25 | epicatechin gallate | inhibits the AroE domain of the bifunctional dehydroquinate dehydratase-shikimate dehydrogenase (DHQ-SDH) from Arabidopsis thaliana | Arabidopsis thaliana | |
1.1.1.25 | epicatechin gallate | over 75% inhibition at 0.0025 mM | Pseudomonas putida | |
1.1.1.25 | epigallocatechin gallate | inhibits the AroE domain of the bifunctional dehydroquinate dehydratase-shikimate dehydrogenase (DHQ-SDH) from Arabidopsis thaliana | Arabidopsis thaliana | |
1.1.1.25 | epigallocatechin gallate | over 75% inhibition at 0.0025 mM | Pseudomonas putida | |
1.1.1.25 | epigallocatechin-3,5-digallate | about 50% inhibition at 0.0025 mM | Pseudomonas putida | |
1.1.1.25 | epitheaflavin monogallate | about 50% inhibition at 0.0025 mM | Pseudomonas putida | |
1.1.1.25 | hydroquinone | lower inhibitry potency, about 25% inhibition at 0.0025 mM | Pseudomonas putida | |
1.1.1.25 | merbromin | lower inhibitry potency, about 25% inhibition at 0.0025 mM | Pseudomonas putida | |
1.1.1.25 | additional information | screening for polyphenolc enzyme inhibitors, overview | Arabidopsis thaliana | |
1.1.1.25 | additional information | screening for polyphenolc enzyme inhibitors, overview. No inhibition by gallic acid, epigallocatechin and epicatechin | Pseudomonas putida | |
1.1.1.25 | nordihydroguaiaretic acid | lower inhibitry potency, about 25% inhibition at 0.0025 mM | Pseudomonas putida | |
1.1.1.25 | purpurogallin | about 50% inhibition at 0.0025 mM | Pseudomonas putida | |
1.1.1.25 | pyridoxine | lower inhibitry potency, about 25% inhibition at 0.0025 mM | Pseudomonas putida | |
1.1.1.25 | pyrogallin | about 50% inhibition at 0.0025 mM | Pseudomonas putida | |
1.1.1.25 | quercetin | about 25% inhibition at 0.0025 mM | Pseudomonas putida | |
1.1.1.25 | taxifolin | about 25% inhibition at 0.0025 mM | Pseudomonas putida | |
1.1.1.25 | theaflavanin | about 25% inhibition at 0.0025 mM | Pseudomonas putida | |
1.1.1.25 | theaflavin monogallate | about 50% inhibition at 0.0025 mM | Pseudomonas putida | |
1.1.1.25 | theaflavin-3,3-digallate | about 25% inhibition at 0.0025 mM | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.25 | 3-dehydroshikimate + NADPH | Arabidopsis thaliana | - |
shikimate + NADP+ | - |
r | |
1.1.1.25 | shikimate + NADP+ | Pseudomonas putida | - |
3-dehydroshikimate + NADPH + H+ | - |
r | |
1.1.1.25 | shikimate + NADP+ | Pseudomonas putida KT 2240 | - |
3-dehydroshikimate + NADPH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.25 | Arabidopsis thaliana | Q9SQT8 | - |
- |
1.1.1.25 | Pseudomonas putida | Q88RQ5 | - |
- |
1.1.1.25 | Pseudomonas putida KT 2240 | Q88RQ5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.25 | recombinant His6-tagged enzyme from Escherichia coli strain BL21 CodonPlus by nickel affinity chromatography and dialysis | Arabidopsis thaliana |
1.1.1.25 | recombinant His6-tagged enzyme from Escherichia coli strain BL21 CodonPlus by nickel affinity chromatography and dialysis | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.25 | 3-dehydroshikimate + NADPH | - |
Arabidopsis thaliana | shikimate + NADP+ | - |
r | |
1.1.1.25 | additional information | The bifunctional enzyme also catalyzes dehydration of 3-dehydroquinate to 3-dehydroshikimate | Arabidopsis thaliana | ? | - |
? | |
1.1.1.25 | shikimate + NADP+ | - |
Pseudomonas putida | 3-dehydroshikimate + NADPH + H+ | - |
r | |
1.1.1.25 | shikimate + NADP+ | - |
Pseudomonas putida KT 2240 | 3-dehydroshikimate + NADPH + H+ | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.25 | AroE | - |
Arabidopsis thaliana |
1.1.1.25 | AroE | - |
Pseudomonas putida |
1.1.1.25 | bifunctional dehydroquinate dehydratase-shikimate dehydrogenase | - |
Arabidopsis thaliana |
1.1.1.25 | DHQ-SDH | - |
Arabidopsis thaliana |
1.1.1.25 | SDH | - |
Arabidopsis thaliana |
1.1.1.25 | SDH | - |
Pseudomonas putida |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.25 | 25 | - |
assay at | Arabidopsis thaliana |
1.1.1.25 | 25 | - |
assay at | Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.25 | 8.8 | - |
assay at | Arabidopsis thaliana |
1.1.1.25 | 8.8 | - |
assay at | Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.25 | NADP+ | - |
Arabidopsis thaliana | |
1.1.1.25 | NADP+ | - |
Pseudomonas putida | |
1.1.1.25 | NADPH | - |
Arabidopsis thaliana | |
1.1.1.25 | NADPH | - |
Pseudomonas putida |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
1.1.1.25 | 0.002 | - |
pH 8.8, 25°C, recombinant enzyme, AroE domain of the DQD-SDH enzyme | Arabidopsis thaliana | epicatechin gallate | |
1.1.1.25 | 0.0021 | - |
pH 8.8, 25°C, recombinant enzyme, AroE domain of the DQD-SDH enzyme | Arabidopsis thaliana | epigallocatechin gallate | |
1.1.1.25 | 0.003 | - |
pH 8.8, 25°C, recombinant enzyme | Pseudomonas putida | epigallocatechin gallate | |
1.1.1.25 | 0.0037 | - |
pH 8.8, 25°C, recombinant enzyme | Pseudomonas putida | epicatechin gallate |