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Literature summary extracted from

  • Christensen, E.M.; Patel, S.M.; Korasick, D.A.; Campbell, A.C.; Krause, K.L.; Becker, D.F.; Tanner, J.J.
    Resolving the cofactor-binding site in the proline biosynthetic enzyme human pyrroline-5-carboxylate reductase 1 (2017), J. Biol. Chem., 292, 7233-7243.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.2
-
 Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.5.1.2 binary complexes of PYCR1 with NADPH or proline, at 1.9 A resolution, and a ternary complex containing NADPH and a P5C/proline analog, to 1.85 A resolution. NADPH is bound to the Rossmann fold. Structures provide a model of the Michaelis complex formed during hydride transfer Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.5.1.2 T238A mutation in conserved residue, about 10fold decrease in catalytic efficiency Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.2 0.07
-
 NADH wild-type, pH 7.5, 37°C Homo sapiens
1.5.1.2 0.159
-
 NADPH mutant T238A, pH 7.5, 37°C Homo sapiens
1.5.1.2 0.283
-
 NADPH wild-type, pH 7.5, 37°C Homo sapiens
1.5.1.2 0.667
-
 1-pyrroline-5-carboxylate cosubstrate NADPH, wild-type, pH 7.5, 37°C Homo sapiens
1.5.1.2 2.887
-
 1-pyrroline-5-carboxylate mutant T238A, wild-type, pH 7.5, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.1.2 mitochondrion
-
 Homo sapiens 5739
-

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.2 Homo sapiens P32322
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.2 1-pyrroline-5-carboxylate + NADH + H+
-
 Homo sapiens L-proline + NAD+
-
 ?
1.5.1.2 1-pyrroline-5-carboxylate + NADPH + H+
-
 Homo sapiens L-proline + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.2 More enzyme forms a concentration-dependent decamer in solution, sedimentation velocity data Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.5.1.2 Pycr1
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.2 14
-
 1-pyrroline-5-carboxylate mutant T238A, wild-type, pH 7.5, 37°C Homo sapiens
1.5.1.2 23
-
 NADPH mutant T238A, pH 7.5, 37°C Homo sapiens
1.5.1.2 31
-
 1-pyrroline-5-carboxylate cosubstrate NADPH, wild-type, pH 7.5, 37°C Homo sapiens
1.5.1.2 74
-
 NADPH wild-type, pH 7.5, 37°C Homo sapiens
1.5.1.2 218
-
 NADH wild-type, pH 7.5, 37°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5.1.2 50
-
 1-pyrroline-5-carboxylate mutant T238A, wild-type, pH 7.5, 37°C Homo sapiens
1.5.1.2 260
-
 NADPH wild-type, pH 7.5, 37°C Homo sapiens
1.5.1.2 470
-
 1-pyrroline-5-carboxylate cosubstrate NADPH, wild-type, pH 7.5, 37°C Homo sapiens
1.5.1.2 1400
-
 NADPH mutant T238A, pH 7.5, 37°C Homo sapiens
1.5.1.2 3100
-
 NADH wild-type, pH 7.5, 37°C Homo sapiens