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Literature summary extracted from
- Spectroscopic and kinetic properties of the molybdenum-containing, NAD+-dependent formate dehydrogenase from Ralstonia eutropha (2016), J. Biol. Chem., 291, 1162-1174.
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.17.1.9 | cytosol | - |
Cupriavidus necator | 5829 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.9 | 178800 | - |
gel filtration | Cupriavidus necator |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.17.1.9 | formate + NAD+ | Cupriavidus necator | - |
CO2 + NADH + H+ | - |
? |  |
| 1.17.1.9 | formate + NAD+ | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | - |
CO2 + NADH + H+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.17.1.9 | Cupriavidus necator | - |
- |
- |
| 1.17.1.9 | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.17.1.9 | ammonium sulfate precipitation, butyl 4-Sepharose column chromatography, Cibacron Blue-agarose 3GA column chromatography, Fractogel TMAE 650 gel filtration, and Superdex 200 gel filtration | Cupriavidus necator |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.17.1.9 | formate + NAD+ | - |
Cupriavidus necator | CO2 + NADH + H+ | - |
? | |
| 1.17.1.9 | formate + NAD+ | - |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | CO2 + NADH + H+ | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.17.1.9 | FdsABG | - |
Cupriavidus necator |
| 1.17.1.9 | NAD+-dependent formate dehydrogenase | - |
Cupriavidus necator |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.9 | 7.5 | - |
- |
Cupriavidus necator |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.1.9 | molybdenum cofactor | - |
Cupriavidus necator | |
| 1.17.1.9 | NAD+ | - |
Cupriavidus necator | |
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Release 2023.1 (January 2023)
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