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Literature summary extracted from

  • Hoeppner, A.; Widderich, N.; Lenders, M.; Bremer, E.; Smits, S.H.
    Crystal structure of the ectoine hydroxylase, a snapshot of the active site (2014), J. Biol. Chem., 289, 29570-29583.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.11.55 crystal structure in its apo-form, in complex with iron, and in complex with iron, cosubstrate 2-oxoglutarate, and 5-hydroxyectoine. The iron and 2-oxoglutarate ligands are bound within the active site in a fashion similar to that found in other members of the dioxygenase superfamily. 5-Hydroxyectoine is bound by residues residues His144, His245, and Asp146 forming the 2-His-1-carboxylate facial triad Sphingopyxis alaskensis

Protein Variants

EC Number Protein Variants Comment Organism
1.14.11.55 E140A residue involved in dimerization, activity similar to wild-type Sphingopyxis alaskensis
1.14.11.55 Q127A residue involved in ectoine binding, about 1% of wild-type activity Sphingopyxis alaskensis
1.14.11.55 R139A residue involved in dimerization, activity similar to wild-type Sphingopyxis alaskensis
1.14.11.55 R139A/E140A residues involved in dimerization, activity similar to wild-type Sphingopyxis alaskensis
1.14.11.55 R280A residue involved in ectoine binding, about 8% of wild-type activity Sphingopyxis alaskensis
1.14.11.55 T149A residue involved in ectoine binding, about 4% of wild-type activity Sphingopyxis alaskensis
1.14.11.55 W150A residue involved in ectoine binding, about 2% of wild-type activity Sphingopyxis alaskensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.55 2.7
-
 2-oxoglutarate pH 8.0, 40°C Sphingopyxis alaskensis
1.14.11.55 9.8
-
 ectoine pH 8.0, 40°C Sphingopyxis alaskensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.55 35290
-
-
 Sphingopyxis alaskensis
1.14.11.55 70730
-
 light scattering, recombinant Strep-tagged protein Sphingopyxis alaskensis

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.55 Sphingopyxis alaskensis Q1GNW5
-
-
1.14.11.55 Sphingopyxis alaskensis DSM 13593 Q1GNW5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.55 ectoine + 2-oxoglutarate + O2
-
 Sphingopyxis alaskensis 5-hydroxyectoine + succinate + CO2
-
 ?
1.14.11.55 ectoine + 2-oxoglutarate + O2
-
 Sphingopyxis alaskensis DSM 13593 5-hydroxyectoine + succinate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.55 dimer 2 * 35290, calculated, recombinant Strep-tagged protein Sphingopyxis alaskensis

Synonyms

EC Number Synonyms Comment Organism
1.14.11.55 Sala_2952
-
 Sphingopyxis alaskensis