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Literature summary extracted from

  • Chen, C.; Tao, T.; Wen, C.; He, W.Q.; Qiao, Y.N.; Gao, Y.Q.; Chen, X.; Wang, P.; Chen, C.P.; Zhao, W.; Chen, H.Q.; Ye, A.P.; Peng, Y.J.; Zhu, M.S.
    Myosin light chain kinase (MLCK) regulates cell migration in a myosin regulatory light chain phosphorylation-independent mechanism (2014), J. Biol. Chem., 289, 28478-28488.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.18 Calmodulin required Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.18 gene Mylk, the MLCK locus encodes three transcripts from alternative promoters and produces two MLCK isoforms (short MLCK and long MLCK) and a C-terminal Ig module (telokin). Long MLCK (L-MLCK) is identical to short MLCK (S-MLCK) apart from a unique N-terminal extension containing two extra DFRXXL motifs and six Ig modules Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.18 additional information generation of enzyme MLCK deletion in intestinal smooth muscle cells, phenotype, overview Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.18 Ca2+ required Mus musculus
2.7.11.18 Mg2+ required Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.18 ATP + [myosin light chain] Mus musculus non-muscle myosin II and preferred substrate smooth muscle myosin II ADP + [myosin light chain] phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.18 Mus musculus Q6PDN3
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.18 intestine
-
 Mus musculus
-
2.7.11.18 smooth muscle cell primary cultured cells Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.18 ATP + [myosin light chain] non-muscle myosin II and preferred substrate smooth muscle myosin II Mus musculus ADP + [myosin light chain] phosphate
-
 ?
2.7.11.18 ATP + [myosin light chain] phosphorylation of Thr-18/Ser-19 on the regulatory light chain of myosin II Mus musculus ADP + [myosin light chain] phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.18 More the MLCK locus encodes three transcripts from alternative promoters and produces two MLCK isoforms (short MLCK and long MLCK) and a C-terminal Ig module (telokin). Long MLCK (L-MLCK) is identical to short MLCK (S-MLCK) apart from a unique N-terminal extension containing two extra DFRXXL motifs and six Ig modules Mus musculus

Synonyms

EC Number Synonyms Comment Organism
2.7.11.18 MLCK
-
 Mus musculus
2.7.11.18 myosin light chain kinase
-
 Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.18 ATP
-
 Mus musculus

General Information

EC Number General Information Comment Organism
2.7.11.18 malfunction MLCK activates myosin Mg-ATPase activity by phosphorylating Thr-18/Ser-19 on the RLC of myosin II, thereby initiating cross-bridge movements of both smooth muscle and non-muscle. MLCK deletion causes enhanced cell protrusion along with a reduction of membrane tension and is rescued by kinase-dead MLCK or five-DFRXXL motif. The deletion of MLCK results in fast cell migration, enhanced protrusion formation, but no alteration of myosin light chain phosphorylation. The mutant cells show reduced membrane tether force and fewer membrane F-actin filaments, as well as a higher average migrating velocity and protrusion compared to wild-type Mus musculus
2.7.11.18 physiological function MLCK regulates cell migration not by myosin regulatory light chain phosphorylation but possibly through a membrane tension-based mechanism, which is a distinct regulatory mechanism of protrusion during cell migration. MLCK is not required for myosin phosphorylation in a migrating cell, but negatively controls migration by maintaining membrane tension. A critical role of MLCK in cell migration involves regulating the cell membrane tension and protrusion necessary for migration, thereby stabilizing the membrane skeleton through F-actin-binding activity. MLCK regulates the membrane F-actin skeleton. MLCK regulates cell spreading through 5DFRXXL in a kinase-independent manner Mus musculus