Osmotically induced synthesis of the compatible solute hydroxyectoine is mediated by an evolutionarily conserved ectoine hydroxylase
Bursy, J.; Pierik, A.J.; Pica, N.; Bremer, E.; J. Biol. Chem. 282, 31147-31155 (2007) 
Data extracted from this reference:
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.11.55
ascorbate
5 mM, 70% inhibition
Virgibacillus salexigens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.11.55
3.5
-
ectoine
pH 7.5, 32°C
Virgibacillus salexigens
1.14.11.55
5.2
-
2-oxoglutarate
pH 7.5, 32°C
Virgibacillus salexigens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.11.55
Fe2+
presence of Fe2+ is required
Virgibacillus salexigens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.11.55
34000
-
-
Virgibacillus salexigens
1.14.11.55
34400
-
-
Virgibacillus salexigens
1.14.11.55
36000
-
gel filtration
Virgibacillus salexigens
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.14.11.55
Virgibacillus salexigens
Q2TDY4
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.14.11.55
-
Virgibacillus salexigens
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.11.55
3.06
-
pH 7.5, 32°C
Virgibacillus salexigens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.14.11.55
ectoine + 2-oxoglutarate + O2
-
740684
Virgibacillus salexigens
5-hydroxyectoine + succinate + CO2
reaction is dependent on iron(II), molecular oxygen, and 2-oxoglutarate
-
-
?
1.14.11.55
additional information
the cosubstrate 2-oxoglutarate is stoichiometrically decarboxylated during the hydroxylation of the substrate ectoine. CO2 is thereby liberated from 2-oxoglutarate to form succinate. During the hydroxylation reaction, one atom of the atmospheric oxygen molecule is incorporated into succinate, whereas the other atom is incorporated into the hydroxy group formed on ectoine
740684
Virgibacillus salexigens
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.14.11.55
monomer
1 * 34000, SDS-PAGE, 1 * 34400, mass spectrometry
Virgibacillus salexigens
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.11.55
32
-
-
Virgibacillus salexigens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.11.55
7.5
-
-
Virgibacillus salexigens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.11.55
ascorbate
5 mM, 70% inhibition
Virgibacillus salexigens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.11.55
3.5
-
ectoine
pH 7.5, 32°C
Virgibacillus salexigens
1.14.11.55
5.2
-
2-oxoglutarate
pH 7.5, 32°C
Virgibacillus salexigens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.11.55
Fe2+
presence of Fe2+ is required
Virgibacillus salexigens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.11.55
34000
-
-
Virgibacillus salexigens
1.14.11.55
34400
-
-
Virgibacillus salexigens
1.14.11.55
36000
-
gel filtration
Virgibacillus salexigens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.11.55
-
Virgibacillus salexigens
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.11.55
3.06
-
pH 7.5, 32°C
Virgibacillus salexigens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.14.11.55
ectoine + 2-oxoglutarate + O2
-
740684
Virgibacillus salexigens
5-hydroxyectoine + succinate + CO2
reaction is dependent on iron(II), molecular oxygen, and 2-oxoglutarate
-
-
?
1.14.11.55
additional information
the cosubstrate 2-oxoglutarate is stoichiometrically decarboxylated during the hydroxylation of the substrate ectoine. CO2 is thereby liberated from 2-oxoglutarate to form succinate. During the hydroxylation reaction, one atom of the atmospheric oxygen molecule is incorporated into succinate, whereas the other atom is incorporated into the hydroxy group formed on ectoine
740684
Virgibacillus salexigens
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.11.55
monomer
1 * 34000, SDS-PAGE, 1 * 34400, mass spectrometry
Virgibacillus salexigens
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.11.55
32
-
-
Virgibacillus salexigens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.11.55
7.5
-
-
Virgibacillus salexigens
Expression
EC Number
Organism
Commentary
Expression
1.14.11.55
Virgibacillus salexigens
ectoine production is linearly correlated with the salinity of the growth medium. The formation of 5-hydroxyectoine is primarily a stationary growth phase phenomenon. The transcript levels of the ectABC and ectD genes increase as a function of salinity
additional information
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.14.11.55
Virgibacillus salexigens
ectoine production is linearly correlated with the salinity of the growth medium. The formation of 5-hydroxyectoine is primarily a stationary growth phase phenomenon. The transcript levels of the ectABC and ectD genes increase as a function of salinity
additional information