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Literature summary extracted from

  • Eliasson, R.; Pontis, E.; Fontecave, M.; Gerez, C.; Harder, J.; Joernvall, H.; Krook, M.; Reichard, P.
    Characterization of components of the anaerobic ribonucleotide reductase system from Escherichia coli (1992), J. Biol. Chem., 267, 25541-25547.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.98.6 ATP strong stimulation Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.98.6 bathophenanthroline 0.02 mM, 97% inhibition Escherichia coli
1.1.98.6 Co2+ 0.02 mM, 75% inhibition Escherichia coli
1.1.98.6 desferal 0.02 mM, 96% inhibition Escherichia coli
1.1.98.6 EDTA 0.1 mM, 96% inhibition Escherichia coli
1.1.98.6 ferrozin 0.1 mM, 72% inhibition Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.98.6 77000
-
 2 * 77000, SDS-PAGE Escherichia coli
1.1.98.6 145000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.1.98.6 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.98.6
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.98.6 260
-
 pH 8.5, 22°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.98.6 ribonucleoside 5'-triphosphate + formate
-
 Escherichia coli 2'-deoxyribonucleoside 5'-triphosphate + CO2 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.98.6 dimer 2 * 77000, SDS-PAGE Escherichia coli
1.1.98.6 More occurence of a degradation product named beta2 of 74000 Da Escherichia coli

General Information

EC Number General Information Comment Organism
1.1.98.6 physiological function the enzyme is the actual ribonucleoside triphosphate reductase in the oxygen-sensitive ribonucleoside triphosphate reductase system. The reaction requires an auxiliary protein dA1 of 29000 Da, S-adenosylmethionine, NADPH (with NADH as a less active substitute), dithinthreitol, and magnesium ions, and is strongly stimulated by ATP Escherichia coli