EC Number | Application | Comment | Organism |
---|---|---|---|
2.7.8.B13 | drug development | the enzyme is a promising target for the development of anti-mycobacterium drugs | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.8.B13 | (+-)-1-deoxy-1-phosphonomethyl-myo-inositol | Ino-C-P, competitive inhibition, 24% and 76% inhibition at 1 mM and 5 mM, respectively | Mycobacterium tuberculosis | |
2.7.8.B13 | (+-)-1-deoxy-1-phosphonomethyl-myo-inositol | leads to growth inhibition | Mycolicibacterium smegmatis | |
2.7.8.B13 | (+-)-1-deoxy-1-[(diethoxyphosphinyl)methyl]-myo-inositol | Ino-C-P(OEt)2, 11% and 40% inhibition at 1 mM and 5 mM, respectively | Mycobacterium tuberculosis | |
2.7.8.B13 | (+-)-1-deoxy-1-[(diethoxyphosphinyl)methyl]-myo-inositol | Ino-C-P(OEt)2, leads to growth inhibition | Mycolicibacterium smegmatis | |
2.7.8.B13 | (+-)-1-O-sulfamoyl-myo-inositol | 16% and 45% inhibition at 1 mM and 5 mM, respectively | Mycobacterium tuberculosis | |
2.7.8.B13 | 1D-myo-inositol 1-monophosphate | 4% and 45% inhibition at 1 mM and 5 mM, respectively | Mycobacterium tuberculosis | |
2.7.8.B13 | 1L-1-deoxy-1-carboxymethyl-chiro-inositol | 7% and 41% inhibition at 1 mM and 5 mM, respectively | Mycobacterium tuberculosis | |
2.7.8.B13 | 1L-1-deoxy-1-carboxymethyl-myo-inositol | 7% and 41% inhibition at 1 mM and 5 mM, respectively | Mycobacterium tuberculosis | |
2.7.8.B13 | additional information | chemical synthesis of four types of inositol 1-phosphate derivatives, all synthesized compounds inhibit PIP synthase activity. The phosphonate analogue of inositol 1-phosphate has the greatest inhibitory effect among the synthesized compounds examined, kinetic analysis. No substrate inhibition by 1L-myo-inositol 1-phosphate | Mycobacterium tuberculosis | |
2.7.8.B13 | additional information | no growth inhibition by (+-)-1-O-sulfamoyl-myo-inositol, 1L-1-deoxy-1-carboxymethyl-myo-inositol, and 1 L-1-deoxy-1-carboxymethyl-chiro-inositol | Mycolicibacterium smegmatis | |
2.7.8.B13 | myo-inositol 2-monophosphate | 10% and 44% inhibition at 1 mM and 5 mM, respectively | Mycobacterium tuberculosis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.8.B13 | Mg2+ | the enzyme requires Mg2+ or Mn2+ | Mycolicibacterium smegmatis | |
2.7.8.B13 | Mg2+ | the enzyme requires Mg2+ or Mn2+ | Mycobacterium tuberculosis | |
2.7.8.B13 | Mn2+ | the enzyme requires Mg2+ or Mn2+ | Mycolicibacterium smegmatis | |
2.7.8.B13 | Mn2+ | the enzyme requires Mg2+ or Mn2+ | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.8.B13 | CDP-diacylglycerol + 1L-myo-inositol 1-phosphate | Mycolicibacterium smegmatis | - |
CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate | - |
? | |
2.7.8.B13 | CDP-diacylglycerol + 1L-myo-inositol 1-phosphate | Mycobacterium tuberculosis | - |
CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.8.B13 | Mycobacterium tuberculosis | - |
- |
- |
2.7.8.B13 | Mycolicibacterium smegmatis | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.8.B13 | CDP-diacylglycerol + 1L-myo-inositol 1-phosphate | - |
Mycolicibacterium smegmatis | CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate | - |
? | |
2.7.8.B13 | CDP-diacylglycerol + 1L-myo-inositol 1-phosphate | - |
Mycobacterium tuberculosis | CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.8.B13 | phosphatidylinositol phosphate synthase | - |
Mycolicibacterium smegmatis |
2.7.8.B13 | phosphatidylinositol phosphate synthase | - |
Mycobacterium tuberculosis |
2.7.8.B13 | PIP synthase | - |
Mycolicibacterium smegmatis |
2.7.8.B13 | PIP synthase | - |
Mycobacterium tuberculosis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.8.B13 | 37 | - |
assay at | Mycobacterium tuberculosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.8.B13 | 8.5 | - |
assay at | Mycobacterium tuberculosis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.8.B13 | metabolism | distinct pathway for the biosynthesis of phosphatidylinositol in mycobacteria via phosphatidylinositol phosphate (PIP) and phosphatidylinositol phosphate synthase activity | Mycolicibacterium smegmatis |
2.7.8.B13 | metabolism | distinct pathway for the biosynthesis of phosphatidylinositol in mycobacteria via phosphatidylinositol phosphate (PIP) and phosphatidylinositol phosphate synthase activity | Mycobacterium tuberculosis |