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Literature summary extracted from

  • Maughan, W.P.; Shuman, S.
    Characterization of 3-phosphate RNA ligase paralogs RtcB1, RtcB2, and RtcB3 from Myxococcus xanthus highlights DNA and RNA 5'-phosphate capping activity of RtcB3 (2015), J. Bacteriol., 197, 3616-3624.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.70
-
 Schizosaccharomyces pombe
6.5.1.8 exprression in Escherichia coli Myxococcus xanthus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.5.1.8 48000
-
 x * 48000, SDS-PAGE Myxococcus xanthus

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.70 Schizosaccharomyces pombe O74859
-
-
3.6.1.70 Schizosaccharomyces pombe 972 O74859
-
-
6.5.1.8 Myxococcus xanthus Q1D2I5
-
-
6.5.1.8 Myxococcus xanthus Q1DFL2
-
-
6.5.1.8 Myxococcus xanthus DK 1622 Q1D2I5
-
-
6.5.1.8 Myxococcus xanthus DK 1622 Q1DFL2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.70 guanosine-5'-diphospho-5'-[DNA] + H2O substrate 12-mer Gpp-DNA strand Schizosaccharomyces pombe phospho-5'-[DNA] + GMP
-
 ?
3.6.1.70 guanosine-5'-diphospho-5'-[DNA] + H2O substrate 12-mer Gpp-DNA strand Schizosaccharomyces pombe 972 phospho-5'-[DNA] + GMP
-
 ?
6.5.1.8 (ribonucleotide)n-3'-phosphate + GTP substrate is a 20-mer HORNAp Myxococcus xanthus ? + GMP RtcB1 efficiently converts the linear substrate to a circular RNA species as a consequence of intramolecular ligation plus multimers ?
6.5.1.8 (ribonucleotide)n-3'-phosphate + GTP substrate is a 20-mer HORNAp Myxococcus xanthus ? + GMP RtcB2 efficiently converts the linear substrate to a circular RNA species as a consequence of intramolecular ligation plus multimers. Isoform Rtcb2 is 5fold less effectively than isoform RtcB1 ?
6.5.1.8 (ribonucleotide)n-3'-phosphate + GTP substrate is a 20-mer HORNAp Myxococcus xanthus DK 1622 ? + GMP RtcB1 efficiently converts the linear substrate to a circular RNA species as a consequence of intramolecular ligation plus multimers ?
6.5.1.8 (ribonucleotide)n-3'-phosphate + GTP substrate is a 20-mer HORNAp Myxococcus xanthus DK 1622 ? + GMP RtcB2 efficiently converts the linear substrate to a circular RNA species as a consequence of intramolecular ligation plus multimers. Isoform Rtcb2 is 5fold less effectively than isoform RtcB1 ?
6.5.1.8 additional information isoform Rtcb1 displays DNA ligase and DNA 3'-capping activities on a 12-mer pDNAp strand Myxococcus xanthus ?
-
 ?
6.5.1.8 additional information isoform Rtcb2 displays DNA ligase and DNA 3'-capping activities on a 12-mer pDNAp strand Myxococcus xanthus ?
-
 ?
6.5.1.8 additional information isoform Rtcb1 displays DNA ligase and DNA 3'-capping activities on a 12-mer pDNAp strand Myxococcus xanthus DK 1622 ?
-
 ?
6.5.1.8 additional information isoform Rtcb2 displays DNA ligase and DNA 3'-capping activities on a 12-mer pDNAp strand Myxococcus xanthus DK 1622 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.5.1.8 ? x * 48000, SDS-PAGE Myxococcus xanthus

Synonyms

EC Number Synonyms Comment Organism
3.6.1.70 aprataxin
-
 Schizosaccharomyces pombe
3.6.1.70 HNT3
-
 Schizosaccharomyces pombe
6.5.1.8 MXAN_0280
-
 Myxococcus xanthus
6.5.1.8 MXAN_4982
-
 Myxococcus xanthus
6.5.1.8 RtcB1
-
 Myxococcus xanthus

General Information

EC Number General Information Comment Organism
3.6.1.70 physiological function 3'-phosphate RNA ligase RtcB3 caps DNA and RNA 5'-PO4 ends to form GppDNA and GppRNA products, respectively. GppDNA formed by RtcB3 can be decapped to pDNA by the DNA repair enzyme aprataxin Schizosaccharomyces pombe