BRENDA - Enzyme Database show

Hydrogen formation and its regulation in Ruminococcus albus: Involvement of an electron-bifurcating [FeFe]-hydrogenase, of a non-electron-bifurcating [FeFe]-hydrogenase, and of a putative hydrogen-sensing [FeFe]-hydrogenase

Zheng, Y.; Kahnt, J.; Kwon, I.H.; Mackie, R.I.; Thauer, R.K.; J. Bacteriol. 196, 3840-3852 (2014)

Data extracted from this reference:

Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.12.1.4
18000
-
-
Ruminococcus albus
1.12.1.4
64000
-
-
Ruminococcus albus
1.12.1.4
68000
-
-
Ruminococcus albus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.12.1.4
Ruminococcus albus
-
-
-
1.12.1.4
Ruminococcus albus DSM 20455
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.12.1.4
-
Ruminococcus albus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.12.1.4
60
-
pH 7.0, 37°C
Ruminococcus albus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.12.1.4
NAD+ + 2 oxidized ferredoxin + 2 H2
-
740625
Ruminococcus albus
NADH + 3 H+ + 2 reduced ferredoxin
-
-
-
?
1.12.1.4
NAD+ + 2 oxidized ferredoxin + 2 H2
-
740625
Ruminococcus albus DSM 20455
NADH + 3 H+ + 2 reduced ferredoxin
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.12.1.4
multimer
x * 68000, subunit HdB, plus x * 64000, subunit HydA, plus x * 18000, subunit HydC
Ruminococcus albus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.12.1.4
additional information
no cofactor: NADP+
Ruminococcus albus
1.12.1.4
NAD+
-
Ruminococcus albus
1.12.1.4
NADH
-
Ruminococcus albus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.12.1.4
additional information
no cofactor: NADP+
Ruminococcus albus
1.12.1.4
NAD+
-
Ruminococcus albus
1.12.1.4
NADH
-
Ruminococcus albus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.12.1.4
18000
-
-
Ruminococcus albus
1.12.1.4
64000
-
-
Ruminococcus albus
1.12.1.4
68000
-
-
Ruminococcus albus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.12.1.4
-
Ruminococcus albus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.12.1.4
60
-
pH 7.0, 37°C
Ruminococcus albus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.12.1.4
NAD+ + 2 oxidized ferredoxin + 2 H2
-
740625
Ruminococcus albus
NADH + 3 H+ + 2 reduced ferredoxin
-
-
-
?
1.12.1.4
NAD+ + 2 oxidized ferredoxin + 2 H2
-
740625
Ruminococcus albus DSM 20455
NADH + 3 H+ + 2 reduced ferredoxin
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.12.1.4
multimer
x * 68000, subunit HdB, plus x * 64000, subunit HydA, plus x * 18000, subunit HydC
Ruminococcus albus