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Literature summary extracted from

  • Tao, Y.; Fishman, A.; Bentley, W.E.; Wood, T.K.
    Altering toluene 4-monooxygenase by active-site engineering for the synthesis of 3-methoxycatechol, methoxyhydroquinone, and methylhydroquinone (2004), J. Bacteriol., 186, 4705-4713.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.236
-
 Pseudomonas mendocina

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.236 G103A/A107S mutation in subunit TmoA, produces 3-methylcatechol (98%) from o-cresol twofold faster and produces 3-methoxycatechol (82%) from 1mM o-methoxyphenol seven times faster than the wild-type Pseudomonas mendocina
1.14.13.236 G103S mutation in subunit TmoA, produces 40fold more methoxyhydroquinone from o-methoxyphenol than the wild-type Pseudomonas mendocina
1.14.13.236 G103S/A107T mutation in subunit TmoA, produces methylhydroquinone (92%) from o-cresol fourfold faster than wild-type Pseudomonas mendocina
1.14.13.236 I100L mutation in subunit TmoA, produces 3-methoxycatechol from o-methoxyphenol four times faster than wild-type Pseudomonas mendocina

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.236 Pseudomonas mendocina Q6Q8Q7 and Q6Q8Q6 and Q6Q8Q5 and Q6Q8Q4 and Q6Q8Q3 and Q6Q8Q2 Q6Q8Q7 i.e. subunit TmoA, Q6Q8Q6 i.e. subunit TmoB, Q6Q8Q5 i.e. subunit TmoC, Q6Q8Q4 i.e. subunit TmoD, Q6Q8Q3 i.e. subunit TmoE, Q6Q8Q2 i.e. subunit TmoF
-
1.14.13.236 Pseudomonas mendocina KR1 Q6Q8Q7 and Q6Q8Q6 and Q6Q8Q5 and Q6Q8Q4 and Q6Q8Q3 and Q6Q8Q2 Q6Q8Q7 i.e. subunit TmoA, Q6Q8Q6 i.e. subunit TmoB, Q6Q8Q5 i.e. subunit TmoC, Q6Q8Q4 i.e. subunit TmoD, Q6Q8Q3 i.e. subunit TmoE, Q6Q8Q2 i.e. subunit TmoF
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.236 m-cresol + NADH + H+ + O2
-
 Pseudomonas mendocina 4-methylcatechol + NAD+ + H2O 100% 4-methylcatechol ?
1.14.13.236 m-cresol + NADH + H+ + O2
-
 Pseudomonas mendocina KR1 4-methylcatechol + NAD+ + H2O 100% 4-methylcatechol ?
1.14.13.236 o-cresol + NADH + H+ + O2
-
 Pseudomonas mendocina 3-methylcatechol + NAD+ + H2O 91% 3-methylcatechol + 9% methylhydroquinone ?
1.14.13.236 o-cresol + NADH + H+ + O2
-
 Pseudomonas mendocina KR1 3-methylcatechol + NAD+ + H2O 91% 3-methylcatechol + 9% methylhydroquinone ?
1.14.13.236 o-methoxyphenol + NADH + H+ + O2
-
 Pseudomonas mendocina 4-methoxyresorcinol + 3-methoxycatechol + NAD+ + H2O 87% 4-methoxyresorcinol, 11% 3-methoxycatechol, and 2% methoxyhydroquinone ?
1.14.13.236 o-methoxyphenol + NADH + H+ + O2
-
 Pseudomonas mendocina KR1 4-methoxyresorcinol + 3-methoxycatechol + NAD+ + H2O 87% 4-methoxyresorcinol, 11% 3-methoxycatechol, and 2% methoxyhydroquinone ?
1.14.13.236 p-cresol + NADH + H+ + O2
-
 Pseudomonas mendocina 4-methylcatechol + NAD+ + H2O 100% 4-methylcatechol ?
1.14.13.236 p-cresol + NADH + H+ + O2
-
 Pseudomonas mendocina KR1 4-methylcatechol + NAD+ + H2O 100% 4-methylcatechol ?
1.14.13.236 toluene + NADH + H+ + O2
-
 Pseudomonas mendocina 4-methylphenol + NAD+ + H2O 96% 4-methylphenol + 3% 3-methylphenol ?
1.14.13.236 toluene + NADH + H+ + O2
-
 Pseudomonas mendocina KR1 4-methylphenol + NAD+ + H2O 96% 4-methylphenol + 3% 3-methylphenol ?