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Literature summary extracted from

  • Masai, E.; Sasaki, M.; Minakawa, Y.; Abe, T.; Sonoki, T.; Miyauchi, K.; Katayama, Y.; Fukuda, M.
    A novel tetrahydrofolate-dependent O-demethylase gene is essential for growth of Sphingomonas paucimobilis SYK-6 with syringate (2004), J. Bacteriol., 186, 2757-2765.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.341 expression in Escherichia coli Sphingomonas paucimobilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.341 50721
-
-
 Sphingomonas paucimobilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.341 syringate + tetrahydrofolate Sphingomonas paucimobilis
-
 3-O-methylgallate + 5-methyltetrahydrofolate
-
 ?
2.1.1.341 syringate + tetrahydrofolate Sphingomonas paucimobilis SYK-6
-
 3-O-methylgallate + 5-methyltetrahydrofolate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.341 Sphingomonas paucimobilis Q7WST1
-
-
2.1.1.341 Sphingomonas paucimobilis SYK-6 Q7WST1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.341 3-O-methylgallate + tetrahydrofolate
-
 Sphingomonas paucimobilis gallate + 5-methyltetrahydrofolate 0.4% of the activity with syringate ?
2.1.1.341 3-O-methylgallate + tetrahydrofolate
-
 Sphingomonas paucimobilis SYK-6 gallate + 5-methyltetrahydrofolate 0.4% of the activity with syringate ?
2.1.1.341 syringate + tetrahydrofolate
-
 Sphingomonas paucimobilis 3-O-methylgallate + 5-methyltetrahydrofolate
-
 ?
2.1.1.341 syringate + tetrahydrofolate
-
 Sphingomonas paucimobilis SYK-6 3-O-methylgallate + 5-methyltetrahydrofolate
-
 ?
2.1.1.341 vanillate + tetrahydrofolate
-
 Sphingomonas paucimobilis protocatechuate + 5-methyltetrahydrofolate 3% of the activity with syringate ?
2.1.1.341 vanillate + tetrahydrofolate
-
 Sphingomonas paucimobilis SYK-6 protocatechuate + 5-methyltetrahydrofolate 3% of the activity with syringate ?

Subunits

EC Number Subunits Comment Organism
2.1.1.341 ? x * 50721, calulated Sphingomonas paucimobilis

Synonyms

EC Number Synonyms Comment Organism
2.1.1.341 DesA
-
 Sphingomonas paucimobilis
2.1.1.341 syringate O-demethylase
-
 Sphingomonas paucimobilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.341 8
-
-
 Sphingomonas paucimobilis

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.341 tetrahydrofolate
-
 Sphingomonas paucimobilis

General Information

EC Number General Information Comment Organism
2.1.1.341 physiological function disruption of DesA results in a growth defect on syringate but does not affect growth on vanillate Sphingomonas paucimobilis