Role of a sensor histidine kinase ChiS of Vibrio cholerae in pathogenesis
Chourashi, R.; Mondal, M.; Sinha, R.; Debnath, A.; Das, S.; Koley, H.; Chatterjee, N.S.; Int. J. Med. Microbiol. 306, 657-665 (2016) 
Data extracted from this reference:
Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.13.3
mucin
the enzyme ChiS is activated 7.4fold in presence of intestinal mucin in vivo and in media supplemented with 2% w/v porcine mucin, the isogenic chiS mutant (ChiS?) shows less growth compared to the wild-type strain ChiS+ in the presence of mucin supplemented media. Activation of ChiS in the host intestine
Vibrio cholerae O1
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.13.3
133000
-
-
Vibrio cholerae O1
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.7.13.3
Vibrio cholerae O1
Q9KUA1
-
-
2.7.13.3
Vibrio cholerae O1 ATCC 39315
Q9KUA1
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.13.3
0.04
-
wild-type strain in absence of mucin, 37°C, pH not specified in the publication
Vibrio cholerae O1
2.7.13.3
0.1805
-
wild-type strain in presence of mucin, 37°C, pH not specified in the publication
Vibrio cholerae O1
Synonyms
EC Number
Synonyms
Commentary
Organism
2.7.13.3
ChiS
-
Vibrio cholerae O1
2.7.13.3
sensor histidine kinase
-
Vibrio cholerae O1
2.7.13.3
VC0622
-
Vibrio cholerae O1
2.7.13.3
VC_0622
-
Vibrio cholerae O1
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.13.3
mucin
the enzyme ChiS is activated 7.4fold in presence of intestinal mucin in vivo and in media supplemented with 2% w/v porcine mucin, the isogenic chiS mutant (ChiS?) shows less growth compared to the wild-type strain ChiS+ in the presence of mucin supplemented media. Activation of ChiS in the host intestine
Vibrio cholerae O1
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.13.3
133000
-
-
Vibrio cholerae O1
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.13.3
0.04
-
wild-type strain in absence of mucin, 37°C, pH not specified in the publication
Vibrio cholerae O1
2.7.13.3
0.1805
-
wild-type strain in presence of mucin, 37°C, pH not specified in the publication
Vibrio cholerae O1
General Information
EC Number
General Information
Commentary
Organism
2.7.13.3
malfunction
the isogenic chiS mutant (ChiS-) shows less growth compared to the wild-type strain ChiS+ in the presence of mucin supplemented media. The mutant ChiS- strain also shows highly retarded motility as well as mucin layer penetration in vitro
Vibrio cholerae O1
2.7.13.3
physiological function
role of a sensor histidine kinase ChiS as an regulator in pathogenesis. The enzyme regulates the chitin utilization pathway and the associated two required factors, chitin binding protein and chitinases, like ChiA2. Enzyme ChiS is important for adherence and survival in HT-29 cells. ChiS is an important factor for utilizing mucin as a sole nutrient source. Cell adhesion, motility, and mucin penetration depends on ChiS. ChiS affects suckling mice colonization in mice and contributes in fluid accumulation as well as colonization in rabbit intestine
Vibrio cholerae O1
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.7.13.3
malfunction
the isogenic chiS mutant (ChiS-) shows less growth compared to the wild-type strain ChiS+ in the presence of mucin supplemented media. The mutant ChiS- strain also shows highly retarded motility as well as mucin layer penetration in vitro
Vibrio cholerae O1
2.7.13.3
physiological function
role of a sensor histidine kinase ChiS as an regulator in pathogenesis. The enzyme regulates the chitin utilization pathway and the associated two required factors, chitin binding protein and chitinases, like ChiA2. Enzyme ChiS is important for adherence and survival in HT-29 cells. ChiS is an important factor for utilizing mucin as a sole nutrient source. Cell adhesion, motility, and mucin penetration depends on ChiS. ChiS affects suckling mice colonization in mice and contributes in fluid accumulation as well as colonization in rabbit intestine
Vibrio cholerae O1