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Literature summary extracted from

  • Chourashi, R.; Mondal, M.; Sinha, R.; Debnath, A.; Das, S.; Koley, H.; Chatterjee, N.S.
    Role of a sensor histidine kinase ChiS of Vibrio cholerae in pathogenesis (2016), Int. J. Med. Microbiol., 306, 657-665.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.13.3 mucin the enzyme ChiS is activated 7.4fold in presence of intestinal mucin in vivo and in media supplemented with 2% w/v porcine mucin, the isogenic chiS mutant (ChiS?) shows less growth compared to the wild-type strain ChiS+ in the presence of mucin supplemented media. Activation of ChiS in the host intestine Vibrio cholerae O1

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.13.3 133000
-
-
 Vibrio cholerae O1

Organism

EC Number Organism UniProt Comment Textmining
2.7.13.3 Vibrio cholerae O1 Q9KUA1
-
-
2.7.13.3 Vibrio cholerae O1 ATCC 39315 Q9KUA1
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.13.3 0.04
-
 wild-type strain in absence of mucin, 37°C, pH not specified in the publication Vibrio cholerae O1
2.7.13.3 0.1805
-
 wild-type strain in presence of mucin, 37°C, pH not specified in the publication Vibrio cholerae O1

Synonyms

EC Number Synonyms Comment Organism
2.7.13.3 ChiS
-
 Vibrio cholerae O1
2.7.13.3 sensor histidine kinase
-
 Vibrio cholerae O1
2.7.13.3 VC0622
-
 Vibrio cholerae O1
2.7.13.3 VC_0622
-
 Vibrio cholerae O1

General Information

EC Number General Information Comment Organism
2.7.13.3 malfunction the isogenic chiS mutant (ChiS-) shows less growth compared to the wild-type strain ChiS+ in the presence of mucin supplemented media. The mutant ChiS- strain also shows highly retarded motility as well as mucin layer penetration in vitro Vibrio cholerae O1
2.7.13.3 physiological function role of a sensor histidine kinase ChiS as an regulator in pathogenesis. The enzyme regulates the chitin utilization pathway and the associated two required factors, chitin binding protein and chitinases, like ChiA2. Enzyme ChiS is important for adherence and survival in HT-29 cells. ChiS is an important factor for utilizing mucin as a sole nutrient source. Cell adhesion, motility, and mucin penetration depends on ChiS. ChiS affects suckling mice colonization in mice and contributes in fluid accumulation as well as colonization in rabbit intestine Vibrio cholerae O1