BRENDA - Enzyme Database

O-antigen structure of Shigella flexneri serotype Yv and effect of the lpt-O gene variation on phosphoethanolamine modification of S. flexneri O-antigens

Knirel, Y.A.; Lan, R.; Senchenkova, S.N.; Wang, J.; Shashkov, A.S.; Wang, Y.; Perepelov, A.V.; Xiong, Y.; Xu, J.; Sun, Q.; Glycobiology 23, 475-485 (2013) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
2.7.8.43
gene lpt-O
Shigella flexneri
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.7.8.43
diacylphosphatidylethanolamine + O-antigen serotype 4av
Shigella flexneri
-
diacylglycerol + O-antigen serotype 4av (2-aminoethyl diphosphate)
the serotype 4av O-antigen has the phosphoethanolamine at position 3 of RhaIII (major) or both RhaII and RhaIII (minor)
-
?
2.7.8.43
diacylphosphatidylethanolamine + O-antigen serotype Xv
Shigella flexneri
-
diacylglycerol + O-antigen serotype Xv (2-aminoethyl diphosphate)
the serotype Xv O-antigen has the phosphoethanolamine on RhaII
-
?
2.7.8.43
diacylphosphatidylethanolamine + O-antigen serotype Yv
Shigella flexneri
-
diacylglycerol + O-antigen serotype Yv 3-(2-aminoethyl diphosphate)
the serotype Yv O-antigen has the same basic carbohydrate backbone structure as that of the classical serotype Y, but differs in the presence of phosphoethanolamine at position 3 of RhaIII (major) or both RhaII and RhaIII (minor)
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.7.8.43
Shigella flexneri
S4UV19
several different strains
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.7.8.43
diacylphosphatidylethanolamine + O-antigen serotype 4av
-
740516
Shigella flexneri
diacylglycerol + O-antigen serotype 4av (2-aminoethyl diphosphate)
the serotype 4av O-antigen has the phosphoethanolamine at position 3 of RhaIII (major) or both RhaII and RhaIII (minor)
-
-
?
2.7.8.43
diacylphosphatidylethanolamine + O-antigen serotype Xv
-
740516
Shigella flexneri
diacylglycerol + O-antigen serotype Xv (2-aminoethyl diphosphate)
the serotype Xv O-antigen has the phosphoethanolamine on RhaII
-
-
?
2.7.8.43
diacylphosphatidylethanolamine + O-antigen serotype Xv
-
740516
Shigella flexneri
diacylglycerol + O-antigen serotype Xv (2-aminoethyl diphosphate)
in serotype Yv, mono- and bisphosphorylated O-units generate a block-copolymeric structure, the former being partially O-acetylated at position 6 of GlcNAc and the latter lacking O-acetylation. The serotype Xv O-antigen has the phosphoethanolamine on RhaII
-
-
?
2.7.8.43
diacylphosphatidylethanolamine + O-antigen serotype Yv
-
740516
Shigella flexneri
diacylglycerol + O-antigen serotype Yv 3-(2-aminoethyl diphosphate)
the serotype Yv O-antigen has the same basic carbohydrate backbone structure as that of the classical serotype Y, but differs in the presence of phosphoethanolamine at position 3 of RhaIII (major) or both RhaII and RhaIII (minor)
-
-
?
2.7.8.43
additional information
NMR spectroscopic analysis of O-antigen substrates and products, detailed overview
740516
Shigella flexneri
?
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
2.7.8.43
lpt-O
-
Shigella flexneri
2.7.8.43
PEtN-transferase
-
Shigella flexneri
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.8.43
gene lpt-O
Shigella flexneri
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.7.8.43
diacylphosphatidylethanolamine + O-antigen serotype 4av
Shigella flexneri
-
diacylglycerol + O-antigen serotype 4av (2-aminoethyl diphosphate)
the serotype 4av O-antigen has the phosphoethanolamine at position 3 of RhaIII (major) or both RhaII and RhaIII (minor)
-
?
2.7.8.43
diacylphosphatidylethanolamine + O-antigen serotype Xv
Shigella flexneri
-
diacylglycerol + O-antigen serotype Xv (2-aminoethyl diphosphate)
the serotype Xv O-antigen has the phosphoethanolamine on RhaII
-
?
2.7.8.43
diacylphosphatidylethanolamine + O-antigen serotype Yv
Shigella flexneri
-
diacylglycerol + O-antigen serotype Yv 3-(2-aminoethyl diphosphate)
the serotype Yv O-antigen has the same basic carbohydrate backbone structure as that of the classical serotype Y, but differs in the presence of phosphoethanolamine at position 3 of RhaIII (major) or both RhaII and RhaIII (minor)
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.7.8.43
diacylphosphatidylethanolamine + O-antigen serotype 4av
-
740516
Shigella flexneri
diacylglycerol + O-antigen serotype 4av (2-aminoethyl diphosphate)
the serotype 4av O-antigen has the phosphoethanolamine at position 3 of RhaIII (major) or both RhaII and RhaIII (minor)
-
-
?
2.7.8.43
diacylphosphatidylethanolamine + O-antigen serotype Xv
-
740516
Shigella flexneri
diacylglycerol + O-antigen serotype Xv (2-aminoethyl diphosphate)
the serotype Xv O-antigen has the phosphoethanolamine on RhaII
-
-
?
2.7.8.43
diacylphosphatidylethanolamine + O-antigen serotype Xv
-
740516
Shigella flexneri
diacylglycerol + O-antigen serotype Xv (2-aminoethyl diphosphate)
in serotype Yv, mono- and bisphosphorylated O-units generate a block-copolymeric structure, the former being partially O-acetylated at position 6 of GlcNAc and the latter lacking O-acetylation. The serotype Xv O-antigen has the phosphoethanolamine on RhaII
-
-
?
2.7.8.43
diacylphosphatidylethanolamine + O-antigen serotype Yv
-
740516
Shigella flexneri
diacylglycerol + O-antigen serotype Yv 3-(2-aminoethyl diphosphate)
the serotype Yv O-antigen has the same basic carbohydrate backbone structure as that of the classical serotype Y, but differs in the presence of phosphoethanolamine at position 3 of RhaIII (major) or both RhaII and RhaIII (minor)
-
-
?
2.7.8.43
additional information
NMR spectroscopic analysis of O-antigen substrates and products, detailed overview
740516
Shigella flexneri
?
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
2.7.8.43
physiological function
in Shigella flexneri serotype X and 4a variants called Xv and 4av, respectively, O-antigen modification with phosphoethanolamine (PEtN) is identified, which is encoded by a plasmid-borne gene lpt-O for a PEtN-transferase and confers the monoclonal antibody IV-1(MASF IV-1) determinant to the bacteria. A correlation between the serotype-specific PEtN modification pattern and the lpt-O variation in different serotypes: lpt-ORII in serotype Xv is better tuned for phosphorylation of RhaII and lpt-ORIII in serotypes Yv and 4av for phosphorylation of RhaII
Shigella flexneri
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.7.8.43
physiological function
in Shigella flexneri serotype X and 4a variants called Xv and 4av, respectively, O-antigen modification with phosphoethanolamine (PEtN) is identified, which is encoded by a plasmid-borne gene lpt-O for a PEtN-transferase and confers the monoclonal antibody IV-1(MASF IV-1) determinant to the bacteria. A correlation between the serotype-specific PEtN modification pattern and the lpt-O variation in different serotypes: lpt-ORII in serotype Xv is better tuned for phosphorylation of RhaII and lpt-ORIII in serotypes Yv and 4av for phosphorylation of RhaII
Shigella flexneri