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Literature summary extracted from

  • Ahmed, N.U.; Park, J.I.; Jung, H.J.; Yang, T.J.; Hur, Y.; Nou, I.S.
    Characterization of dihydroflavonol 4-reductase (DFR) genes and their association with cold and freezing stress in Brassica rapa (2014), Gene, 550, 46-55.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.219 gene BrDFR1, on chromosome A02, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis Brassica rapa subsp. pekinensis
1.1.1.219 gene BrDFR10, on chromosome A06, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis Brassica rapa subsp. pekinensis
1.1.1.219 gene BrDFR11, on chromosome A03, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis Brassica rapa subsp. pekinensis
1.1.1.219 gene BrDFR12, on chromosome A08, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis Brassica rapa subsp. pekinensis
1.1.1.219 gene BrDFR2, on chromosome A09, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis Brassica rapa subsp. pekinensis
1.1.1.219 gene BrDFR3, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis Brassica rapa subsp. pekinensis
1.1.1.219 gene BrDFR3, on chromosome A02, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis Brassica rapa subsp. pekinensis
1.1.1.219 gene BrDFR4, on chromosome A09, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis Brassica rapa subsp. pekinensis
1.1.1.219 gene BrDFR5, on chromosome A09, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis Brassica rapa subsp. pekinensis
1.1.1.219 gene BrDFR6, on chromosome A09, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis Brassica rapa subsp. pekinensis
1.1.1.219 gene BrDFR7, on chromosome A09, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis Brassica rapa subsp. pekinensis
1.1.1.219 gene BrDFR8, on chromosome A06, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis Brassica rapa subsp. pekinensis
1.1.1.219 gene BrDFR9, on chromosome A06, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis Brassica rapa subsp. pekinensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.219 34762
-
-
Brassica rapa subsp. pekinensis
1.1.1.219 34860
-
-
Brassica rapa subsp. pekinensis
1.1.1.219 35420
-
-
Brassica rapa subsp. pekinensis
1.1.1.219 35520
-
-
Brassica rapa subsp. pekinensis
1.1.1.219 35860
-
-
Brassica rapa subsp. pekinensis
1.1.1.219 35910
-
-
Brassica rapa subsp. pekinensis
1.1.1.219 36158
-
-
Brassica rapa subsp. pekinensis
1.1.1.219 36320
-
-
Brassica rapa subsp. pekinensis
1.1.1.219 39650
-
-
Brassica rapa subsp. pekinensis
1.1.1.219 40450
-
-
Brassica rapa subsp. pekinensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.219 dihydrokaempferol + NADPH + H+ Brassica rapa subsp. pekinensis
-
leucopelargonidin + NADP+
-
?
1.1.1.219 dihydromyricetin + NADPH + H+ Brassica rapa subsp. pekinensis
-
leucodelphinidin + NADP+
-
?
1.1.1.219 dihydroquercetin + NADPH + H+ Brassica rapa subsp. pekinensis
-
cis-3,4-leucocyanidin + NADP+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.219 Brassica rapa subsp. pekinensis M4D235 cvs. Palgangbaechu, 8053, and Seoulbaechu
-
1.1.1.219 Brassica rapa subsp. pekinensis M4DRB9 cvs. Palgangbaechu, 8053, and Seoulbaechu
-
1.1.1.219 Brassica rapa subsp. pekinensis M4DU26 cvs. Palgangbaechu, 8053, and Seoulbaechu
-
1.1.1.219 Brassica rapa subsp. pekinensis M4DU27 cvs. Palgangbaechu, 8053, and Seoulbaechu
-
1.1.1.219 Brassica rapa subsp. pekinensis M4DU28 cvs. Palgangbaechu, 8053, and Seoulbaechu
-
1.1.1.219 Brassica rapa subsp. pekinensis M4E380 cvs. Palgangbaechu, 8053, and Seoulbaechu
-
1.1.1.219 Brassica rapa subsp. pekinensis M4E7I9 cvs. Palgangbaechu, 8053, and Seoulbaechu
-
1.1.1.219 Brassica rapa subsp. pekinensis M4E7J1 cvs. Palgangbaechu, 8053, and Seoulbaechu
-
1.1.1.219 Brassica rapa subsp. pekinensis M4E7J2 cvs. Palgangbaechu, 8053, and Seoulbaechu
-
1.1.1.219 Brassica rapa subsp. pekinensis M4ECU0 cvs. Palgangbaechu, 8053, and Seoulbaechu
-
1.1.1.219 Brassica rapa subsp. pekinensis M4ES88 cvs. Palgangbaechu, 8053, and Seoulbaechu
-
1.1.1.219 Brassica rapa subsp. pekinensis M4FB76 cvs. Palgangbaechu, 8053, and Seoulbaechu
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.219 flower bud
-
Brassica rapa subsp. pekinensis
-
1.1.1.219 flower bud high expression level Brassica rapa subsp. pekinensis
-
1.1.1.219 leaf
-
Brassica rapa subsp. pekinensis
-
1.1.1.219 additional information organ-specific DFR expression analysis Brassica rapa subsp. pekinensis
-
1.1.1.219 root
-
Brassica rapa subsp. pekinensis
-
1.1.1.219 stem
-
Brassica rapa subsp. pekinensis
-
1.1.1.219 stem weak expression Brassica rapa subsp. pekinensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.219 dihydrokaempferol + NADPH + H+
-
Brassica rapa subsp. pekinensis leucopelargonidin + NADP+
-
?
1.1.1.219 dihydromyricetin + NADPH + H+
-
Brassica rapa subsp. pekinensis leucodelphinidin + NADP+
-
?
1.1.1.219 dihydroquercetin + NADPH + H+
-
Brassica rapa subsp. pekinensis cis-3,4-leucocyanidin + NADP+
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.219 ? x * 34760, sequence calculation Brassica rapa subsp. pekinensis
1.1.1.219 ? x * 34860, sequence calculation Brassica rapa subsp. pekinensis
1.1.1.219 ? x * 35420, sequence calculation Brassica rapa subsp. pekinensis
1.1.1.219 ? x * 35520, sequence calculation Brassica rapa subsp. pekinensis
1.1.1.219 ? x * 35860, sequence calculation Brassica rapa subsp. pekinensis
1.1.1.219 ? x * 35910, sequence calculation Brassica rapa subsp. pekinensis
1.1.1.219 ? x * 36150, sequence calculation Brassica rapa subsp. pekinensis
1.1.1.219 ? x * 36320, sequence calculation Brassica rapa subsp. pekinensis
1.1.1.219 ? x * 39650, sequence calculation Brassica rapa subsp. pekinensis
1.1.1.219 ? x * 40450, sequence calculation Brassica rapa subsp. pekinensis
1.1.1.219 More sequence analysis and structure homology, overview Brassica rapa subsp. pekinensis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.219 BrDFR1
-
Brassica rapa subsp. pekinensis
1.1.1.219 BrDFR10
-
Brassica rapa subsp. pekinensis
1.1.1.219 BrDFR11
-
Brassica rapa subsp. pekinensis
1.1.1.219 BrDFR12
-
Brassica rapa subsp. pekinensis
1.1.1.219 BrDFR2
-
Brassica rapa subsp. pekinensis
1.1.1.219 BrDFR3
-
Brassica rapa subsp. pekinensis
1.1.1.219 BrDFR4
-
Brassica rapa subsp. pekinensis
1.1.1.219 BrDFR5
-
Brassica rapa subsp. pekinensis
1.1.1.219 BrDFR6
-
Brassica rapa subsp. pekinensis
1.1.1.219 BrDFR7
-
Brassica rapa subsp. pekinensis
1.1.1.219 BrDFR8
-
Brassica rapa subsp. pekinensis
1.1.1.219 BrDFR9
-
Brassica rapa subsp. pekinensis
1.1.1.219 DFR
-
Brassica rapa subsp. pekinensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.219 NADPH
-
Brassica rapa subsp. pekinensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.219 Brassica rapa subsp. pekinensis sequence calculation
-
5.2
1.1.1.219 Brassica rapa subsp. pekinensis sequence calculation
-
5.21
1.1.1.219 Brassica rapa subsp. pekinensis sequence calculation
-
5.26
1.1.1.219 Brassica rapa subsp. pekinensis sequence calculation
-
5.36
1.1.1.219 Brassica rapa subsp. pekinensis sequence calculation
-
5.41
1.1.1.219 Brassica rapa subsp. pekinensis sequence calculation
-
5.45
1.1.1.219 Brassica rapa subsp. pekinensis sequence calculation
-
5.49
1.1.1.219 Brassica rapa subsp. pekinensis sequence calculation
-
5.66
1.1.1.219 Brassica rapa subsp. pekinensis sequence calculation
-
5.9
1.1.1.219 Brassica rapa subsp. pekinensis sequence calculation
-
6.09
1.1.1.219 Brassica rapa subsp. pekinensis sequence calculation
-
6.2
1.1.1.219 Brassica rapa subsp. pekinensis sequence calculation
-
8.65

Expression

EC Number Organism Comment Expression
1.1.1.219 Brassica rapa subsp. pekinensis induction of gene BrDFR10 by cold stress dependent on the genotypes, overview up
1.1.1.219 Brassica rapa subsp. pekinensis induction of gene BrDFR2 by cold stress dependent on the genotypes, overview up
1.1.1.219 Brassica rapa subsp. pekinensis induction of gene BrDFR3 by cold stress dependent on the genotypes, overview up
1.1.1.219 Brassica rapa subsp. pekinensis induction of gene BrDFR4 by cold stress dependent on the genotypes, overview up
1.1.1.219 Brassica rapa subsp. pekinensis induction of gene BrDFR5 by cold stress dependent on the genotypes, overview up
1.1.1.219 Brassica rapa subsp. pekinensis induction of gene BrDFR6 by cold stress dependent on the genotypes, overview up
1.1.1.219 Brassica rapa subsp. pekinensis induction of gene BrDFR8 by cold stress dependent on the genotypes, overview up
1.1.1.219 Brassica rapa subsp. pekinensis induction of gene BrDFR9 by cold stress dependent on the genotypes, overview up

General Information

EC Number General Information Comment Organism
1.1.1.219 physiological function BrDFR isozymes are regulated by two transcription factors, BrMYB2-2 and BrTT8, contrasting with anthocyanin accumulation and cold and freezing stress Brassica rapa subsp. pekinensis