Literature summary extracted from
Singh, D.; Kwon, H.J.; Rajkarnikar, A.; Suh, J.W.
Glucoamylase gene, vldI, is linked to validamycin biosynthesis in Streptomyces hygroscopicus var. limoneus, and vldADEFG confers validamycin production in Streptomyces lividans, revealing the role of VldE in glucose attachment (2007), Gene, 395, 151-159.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.4.1.338 |
expressed in Escherichia coli BL21(DE3) cells |
Streptomyces hygroscopicus |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.4.1.338 |
56000 |
- |
x * 56000, calculated from amino acid sequence |
Streptomyces hygroscopicus |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
2.4.1.338 |
UDP-alpha-D-glucose + validoxylamine A |
Streptomyces hygroscopicus |
- |
UDP + validamycin A |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.4.1.338 |
Streptomyces hygroscopicus |
Q15JG1 |
variant limoneus |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.4.1.338 |
nickel affinity column chromatography, and Superdex 200 gel filtration |
Streptomyces hygroscopicus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.4.1.338 |
UDP-alpha-D-glucose + validoxylamine A |
- |
Streptomyces hygroscopicus |
UDP + validamycin A |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.4.1.338 |
? |
x * 56000, calculated from amino acid sequence |
Streptomyces hygroscopicus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.4.1.338 |
vldE |
- |
Streptomyces hygroscopicus |