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Literature summary extracted from

  • Kawahigashi, H.; Kasuga, S.; Sawada, Y.; Yonemaru, J.; Ando, T.; Kanamori, H.; Wu, J.; Mizuno, H.; Momma, M.; Fujimoto, Z.; Hirai, M.Y.; Matsumoto, T.
    The sorghum gene for leaf color changes upon wounding (P) encodes a flavanone 4-reductase in the 3-deoxyanthocyanidin biosynthesis pathway (2016), G3 (Bethesda), 6, 1439-1447.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.234 gene P or Sb06g029550, quantitative RT-PCR enzyme expression analysis, genetic mapping, phylogenetic analysis and tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 Star(DE3)pLysS Sorghum bicolor

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.234 C252Y cultivar Greenleaf Sb06g029550 has a point mutation that causes the amino acid change Sorghum bicolor
1.1.1.234 additional information analysis of Sb06g029550 in four sorghum accessions with the tan phenotype, overview Sorghum bicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.234 37800
-
-
 Sorghum bicolor
1.1.1.234 40000
-
-
 Sorghum bicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.234 (2S)-flavan-4-ol + NADP+ Sorghum bicolor
-
 (2S)-flavanone + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.234 Sorghum bicolor C5YGL7 purple Nakei-MS3B (PP) and tan Greenleaf (pp) cultivars
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.234 recombinant His-tagged enzyme from Escherichia coli strain BL21 Star(DE3)pLysS by nickel affinity chromatography Sorghum bicolor

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.234 leaf
-
 Sorghum bicolor
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.234 (2S)-flavan-4-ol + NADP+
-
 Sorghum bicolor (2S)-flavanone + NADPH + H+
-
 ?
1.1.1.234 eriodictyol + NADP+ recombinant Sb06g029550 protein Sorghum bicolor luteoforol + NADPH + H+
-
 ?
1.1.1.234 additional information the reaction mixtures are acid-treated to convert the direct reaction products, flavan-3,4-diol, and flavan-4-ol, to their respective anthocyanidin or 3-deoxyanthocyanidins, and analyzed by LC-MS/MS Sorghum bicolor ?
-
 ?
1.1.1.234 naringenin + NADPH + H+ recombinant Sb06g029550 protein Sorghum bicolor apiforol + NADP+ apiforol is converted to apigeninidin with Nar + protein + HCl treatment ?

Subunits

EC Number Subunits Comment Organism
1.1.1.234 ? x * 40000, recombinant His-tagged enzyme, SDS-PAGE, x * 37800, about, sequence calculation Sorghum bicolor

Synonyms

EC Number Synonyms Comment Organism
1.1.1.234 3-deoxyanthocyanidin synthase
-
 Sorghum bicolor
1.1.1.234 FNR
-
 Sorghum bicolor
1.1.1.234 Sb06g029550
-
 Sorghum bicolor
1.1.1.234 Sb06g029550 protein
-
 Sorghum bicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.234 30
-
 assay at Sorghum bicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.234 7
-
 assay at Sorghum bicolor

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.234 NADP+
-
 Sorghum bicolor

Expression

EC Number Organism Comment Expression
1.1.1.234 Sorghum bicolor the enzyme is highly induced by wounding and pathogen invasion, analysis of 3-deoxyanthocyanidin accumulation up

General Information

EC Number General Information Comment Organism
1.1.1.234 metabolism the enzyme, encoded by gene Sb06g029550, is involved in the 3-deoxyanthocyanidin synthesis pathway Sorghum bicolor
1.1.1.234 additional information homology modeling on the basis of the crystal structure of grape dihydroflavonol 4-reductase, PDB ID 3C1T Sorghum bicolor
1.1.1.234 physiological function upon wounding or pathogen invasion, leaves of Sorghum bicolor plants with the P gene turn purple, whereas leaves with the recessive allele turn brown or tan. This purple phenotype is determined by the production of two 3-deoxyanthocyanidins, apigeninidin and luteolinidin, which are not produced by the tan-phenotype plants Sorghum bicolor