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Literature summary extracted from

  • Ibrahim, I.M.; Puthiyaveetil, S.; Allen, J.F.
    A two-component regulatory system in transcriptional control of photosystem stoichiometry: redox-dependent and sodium ion-dependent phosphoryl transfer from cyanobacterial histidine kinase Hik2 to response regulators Rre1 and RppA (2016), Front. Plant Sci., 7, 137.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.13.3 gene slr1147, recombinant expression of C-terminally poly-His-tagged full-length enzyme in Escherichia coli strain BL21(DE3) Synechocystis sp. PCC 6803

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.13.3 additional information upon salt and/or hyperosmotic signal, the activity of Hik2 is inhibited. KCl does not inhibit the autophosphorylation activity of Hik2 Synechocystis sp. PCC 6803
2.7.13.3 Na+ strongly inhibits the autophosphorylation activity of Hik2. 0.5 M NaCl reduces the organism's growth rate by 50% Synechocystis sp. PCC 6803

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.13.3 cytoplasm
-
 Synechocystis sp. PCC 6803 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.13.3 ATP + protein L-histidine Synechocystis sp. PCC 6803
-
 ADP + protein N-phospho-L-histidine
-
 ?
2.7.13.3 additional information Synechocystis sp. PCC 6803 interaction of Hik2 with Rre1 and RppA, usage of recombinant N-terminally MBP-tagged proteins purified from Escherichia coli expression host ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.13.3 Synechocystis sp. PCC 6803 P73276
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.13.3 phosphoprotein Hik2 undergoes autophosphorylation on a conserved histidine residue, at pH 7.5 and 22°C, and becomes rapidly dephosphorylated by the action of response regulators Rre1 and RppA (regulator of phycobilisome association), at pH 7.5 and 30°C. The autophosphorylation of Hik2 is specifically inhibited by sodium ions Synechocystis sp. PCC 6803

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.13.3 recombinant C-terminally poly-His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration. Overexpression of te GST-tagged enzyme in a bait and prey two hybrid system in Saccharomyces cerevisiae Synechocystis sp. PCC 6803

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.13.3 ATP + protein L-histidine
-
 Synechocystis sp. PCC 6803 ADP + protein N-phospho-L-histidine
-
 ?
2.7.13.3 additional information interaction of Hik2 with Rre1 and RppA, usage of recombinant N-terminally MBP-tagged proteins purified from Escherichia coli expression host Synechocystis sp. PCC 6803 ?
-
 ?
2.7.13.3 additional information during autophosphorylation, histidine kinases catalyze transfer of only the gamma-phosphate from an ATP molecule to their conserved histidine residue Synechocystis sp. PCC 6803 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.13.3 Hik2
-
 Synechocystis sp. PCC 6803
2.7.13.3 histidine kinase
-
 Synechocystis sp. PCC 6803
2.7.13.3 sensory transduction histidine kinase UniProt Synechocystis sp. PCC 6803
2.7.13.3 slr1147
-
 Synechocystis sp. PCC 6803

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.13.3 ATP the ATP-binding domain of histidine kinases contains conserved motifs essential for autokinase activity. These include G1 and G2 boxes, which have the characteristic glycine signatures DxGxG and GxGxG, respectively Synechocystis sp. PCC 6803

General Information

EC Number General Information Comment Organism
2.7.13.3 metabolism proposed signal transduction pathway for the Hik2-based two-component system in cyanobacteria, overview Synechocystis sp. PCC 6803
2.7.13.3 physiological function Hik2 is the cytoplasmic homologue of chloroplast sensor kinase (CSK), a protein involved in redox regulation of chloroplast gene expression during changes in light quality in plants and algae. Hik2 is the sensor that acts on Rre1 and RppA transcription factors to regulate photosynthesis genes as part of the mechanism of photosystem stoichiometry adjustment in cyanobacteria. Signaling mechanism of Hik2 and its phosphotransferase activity, overview. Hik2-based signaling pathway integrates acclimatory responses to light and salt stresses in cyanobacteria Synechocystis sp. PCC 6803