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Literature summary extracted from

  • Forlani, G.; Bertazzini, M.; Zarattini, M.; Funck, D.
    Functional characterization and expression analysis of rice delta(1)-pyrroline-5-carboxylate dehydrogenase provide new insight into the regulation of proline and arginine catabolism (2015), Front. Plant Sci., 6, 591.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.88 expression in Escherichia coli Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.88 Cd2+
-
 Oryza sativa
1.2.1.88 Cu2+
-
 Oryza sativa
1.2.1.88 L-arginine uncompetitive Oryza sativa
1.2.1.88 Mn2+
-
 Oryza sativa
1.2.1.88 Zn2+
-
 Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.88 0.265
-
 1-pyrroline-5-carboxylate cosubstrate NADP+, pH 7.5, 35°C Oryza sativa
1.2.1.88 0.358
-
 1-pyrroline-5-carboxylate cosubstrate NAD+, pH 7.5, 35°C Oryza sativa
1.2.1.88 0.644
-
 NAD+ pH 7.5, 35°C Oryza sativa
1.2.1.88 4.695
-
 NAD+ pH 7.5, 35°C Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.88 58200
-
-
 Oryza sativa
1.2.1.88 259000
-
 gel filtration Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.88 Oryza sativa
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.88 1-pyrroline-5-carboxylate + NAD+ + H2O
-
 Oryza sativa L-glutamate + NADH
-
 ?
1.2.1.88 1-pyrroline-5-carboxylate + NADP+ + H2O
-
 Oryza sativa L-glutamate + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.88 tetramer 4 * 58200, SDS-PAGE Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.88 46
-
-
 Oryza sativa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.88 47.5
-
 5 min, 50% loss of activity Oryza sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.88 3
-
 NADP+ pH 7.5, 35°C Oryza sativa
1.2.1.88 12
-
 NAD+ pH 7.5, 35°C Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.88 6.7
-
-
 Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.88 NAD+ NAD+ is preferred over NADP+ Oryza sativa
1.2.1.88 NADP+ NAD+ is preferred over NADP+ Oryza sativa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.2.1.88 Oryza sativa isoelectric focusing
-
 5.6

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.2.1.88 0.000022
-
 pH 7.5, 35°C Oryza sativa Cd2+
1.2.1.88 0.0015
-
 pH 7.5, 35°C Oryza sativa Zn2+
1.2.1.88 0.0024
-
 pH 7.5, 35°C Oryza sativa Mn2+
1.2.1.88 0.004
-
 pH 7.5, 35°C Oryza sativa Cu2+