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Literature summary extracted from

  • Akita, H.; Watanabe, M.; Suzuki, T.; Nakashima, N.; Hoshino, T.
    Molecular cloning and characterization of two YGL039w genes encoding broad specificity NADPH-dependent aldehyde reductases from Kluyveromyces marxianus strain DMB1 (2015), FEMS Microbiol. Lett., 362, pii: fnv116.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.184 expression in Escherichia coli Kluyveromyces marxianus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.184 0.028
-
 NADPH pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.184 0.034
-
 NADPH pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.184 0.43
-
 methyl glyoxal pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.184 1.6
-
 methyl glyoxal pH 6.0, 25°C Kluyveromyces marxianus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.184 35800
-
 gel filtration Kluyveromyces marxianus
1.1.1.184 36900
-
 gel filtration Kluyveromyces marxianus
1.1.1.184 37000
-
 1 * 37000, SDS-PAGE Kluyveromyces marxianus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.184 Kluyveromyces marxianus A0A0E4AX59
-
-
1.1.1.184 Kluyveromyces marxianus A0A0E4AY21
-
-
1.1.1.184 Kluyveromyces marxianus DMB1 A0A0E4AX59
-
-
1.1.1.184 Kluyveromyces marxianus DMB1 A0A0E4AY21
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.184 0.58
-
 substrate benzaldehyde, pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.184 0.6
-
 substrate benzaldehyde, pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.184 0.65
-
 substrate furfural, pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.184 0.96
-
 substrate methyl glyoxal, pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.184 1.1
-
 substrate p-anisaldehyde, pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.184 1.2
-
 substrate furfural, pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.184 1.5
-
 substrate hexanal, pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.184 2
-
 substrate hexanal, pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.184 2.1
-
 substrate octanal, pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.184 2.1
-
 substrate valeraldehyde, pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.184 3
-
 substrate heptanal, pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.184 3.2
-
 substrate methyl glyoxal, pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.184 3.3
-
 substrate isovaleraldehyde, pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.184 4.2
-
 substrate isovaleraldehyde, pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.184 4.5
-
 substrate p-anisaldehyde, pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.184 5.3
-
 substrate octanal, pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.184 5.9
-
 substrate valeraldehyde, pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.184 6.9
-
 substrate heptanal, pH 6.0, 25°C Kluyveromyces marxianus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.184 benzaldehyde + NADPH + H+
-
 Kluyveromyces marxianus benzyl alcohol + NADP+
-
 ?
1.1.1.184 benzaldehyde + NADPH + H+
-
 Kluyveromyces marxianus DMB1 benzyl alcohol + NADP+
-
 ?
1.1.1.184 furfural + NADPH + H+
-
 Kluyveromyces marxianus furfuryl alcohol + NADP+
-
 ?
1.1.1.184 heptanal + NADPH + H+
-
 Kluyveromyces marxianus heptan-1-ol + NADP+
-
 ?
1.1.1.184 heptanal + NADPH + H+
-
 Kluyveromyces marxianus DMB1 heptan-1-ol + NADP+
-
 ?
1.1.1.184 hexanal + NADPH + H+
-
 Kluyveromyces marxianus hexan-1-ol + NADP+
-
 ?
1.1.1.184 isovaleraldehyde + NADPH + H+
-
 Kluyveromyces marxianus isoamyl alcohol + NADP+
-
 ?
1.1.1.184 isovaleraldehyde + NADPH + H+
-
 Kluyveromyces marxianus DMB1 isoamyl alcohol + NADP+
-
 ?
1.1.1.184 methyl glyoxal + NADPH + H+
-
 Kluyveromyces marxianus ? + NADP+
-
 ?
1.1.1.184 methyl glyoxal + NADPH + H+
-
 Kluyveromyces marxianus DMB1 ? + NADP+
-
 ?
1.1.1.184 additional information strong activities are observed toward linear aldehydes, such as 1-heptanal, valeraldehyde and 1-octanal, but no activity toward 5-hydroxymethylfurfural, propionaldehyde, D-alanine, L-alanine,D-lactate, L-lactate or pyruvate. Enzyme does not exhibit NADP+-dependent oxidative activity toward corresponding alcohol analogs, including 1-hexanol, 1-heptanol, isoamyl alcohol, isobutanol, 1-octanol and 2-propanol Kluyveromyces marxianus ?
-
 ?
1.1.1.184 additional information strong activities are observed toward linear aldehydes, such as 1-heptanal, valeraldehyde and 1-octanal, but no activity toward HMF, propionaldehyde, D-alanine, L-alanine,D-lactate, L-lactate or pyruvate. Enzyme does not exhibit NADP+-dependent oxidative activity toward corresponding alcohol analogs, including 1-hexanol, 1-heptanol, isoamyl alcohol, isobutanol, 1-octanol and 2-propanol Kluyveromyces marxianus ?
-
 ?
1.1.1.184 additional information strong activities are observed toward linear aldehydes, such as 1-heptanal, valeraldehyde and 1-octanal, but no activity toward 5-hydroxymethylfurfural, propionaldehyde, D-alanine, L-alanine,D-lactate, L-lactate or pyruvate. Enzyme does not exhibit NADP+-dependent oxidative activity toward corresponding alcohol analogs, including 1-hexanol, 1-heptanol, isoamyl alcohol, isobutanol, 1-octanol and 2-propanol Kluyveromyces marxianus DMB1 ?
-
 ?
1.1.1.184 additional information strong activities are observed toward linear aldehydes, such as 1-heptanal, valeraldehyde and 1-octanal, but no activity toward HMF, propionaldehyde, D-alanine, L-alanine,D-lactate, L-lactate or pyruvate. Enzyme does not exhibit NADP+-dependent oxidative activity toward corresponding alcohol analogs, including 1-hexanol, 1-heptanol, isoamyl alcohol, isobutanol, 1-octanol and 2-propanol Kluyveromyces marxianus DMB1 ?
-
 ?
1.1.1.184 octanal + NADPH + H+
-
 Kluyveromyces marxianus octan-1-ol + NADP+
-
 ?
1.1.1.184 p-anisaldehyde + NADPH + H+
-
 Kluyveromyces marxianus p-anisalcohol + NADP+
-
 ?
1.1.1.184 valeraldehyde + NADPH + H+
-
 Kluyveromyces marxianus pentan-1-ol + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.184 monomer 1 * 37000, SDS-PAGE Kluyveromyces marxianus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.184 YGL039w1
-
 Kluyveromyces marxianus
1.1.1.184 YGL039w2
-
 Kluyveromyces marxianus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.184 45 50
-
 Kluyveromyces marxianus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.184 45
-
 20 min, loss of more than 50% of activity Kluyveromyces marxianus
1.1.1.184 47
-
 melting temperature Kluyveromyces marxianus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.184 2.5
-
 methyl glyoxal pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.184 2.67
-
 NADPH pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.184 3.67
-
 NADPH pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.184 4
-
 methyl glyoxal pH 6.0, 25°C Kluyveromyces marxianus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.184 6
-
-
 Kluyveromyces marxianus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.184 additional information no activity with NADH Kluyveromyces marxianus
1.1.1.184 NADPH exclusive use of NADPH Kluyveromyces marxianus