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Literature summary extracted from

  • Loose, J.; Forsberg, Z.; Fraaije, M.; Eijsink, V.; Vaaje-Kolstad, G.
    A rapid quantitative activity assay shows that the Vibrio cholerae colonization factor GbpA is an active lytic polysaccharide monooxygenase (2014), FEBS Lett., 588, 3435-3440.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.99.53 analysis method for quantification of C1-oxidized chitooligosaccharides (aldonic acids), and hence LPMO activity Vibrio cholerae serotype O1

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.53 expression in Escherichia coli Vibrio cholerae serotype O1

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.99.53 Cu2+ saturation of enzyme with cu2+ prior to assay Vibrio cholerae serotype O1

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.53 Vibrio cholerae serotype O1
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.53 beta-chitin + ascorbate + O2
-
 Vibrio cholerae serotype O1 C1-oxidized chitooligosaccharides + dehydroascorbate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.99.53 GbpA virulence factor with no obvious role in biomass processing Vibrio cholerae serotype O1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.99.53 37
-
 assay at Vibrio cholerae serotype O1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.99.53 6.8
-
 assay at Vibrio cholerae serotype O1