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Literature summary extracted from

  • Ewing, T.A.; Gygli, G.; van Berkel, W.J.
    A single loop is essential for the octamerization of vanillyl alcohol oxidase (2016), FEBS J., 283, 2546-2559.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.38 expressed in Escherichia coli BL21 cells Penicillium simplicissimum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.38 0.0039
-
 eugenol wild type enzyme, at pH 7.5 and 25°C Penicillium simplicissimum
1.1.3.38 0.012
-
 4-Ethylphenol wild type enzyme, at pH 7.5 and 25°C Penicillium simplicissimum
1.1.3.38 0.149
-
 Vanillyl alcohol wild type enzyme, at pH 7.5 and 25°C Penicillium simplicissimum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.38 61000
-
 8 * 61000, SDS-PAGE Penicillium simplicissimum
1.1.3.38 64000
-
-
 Penicillium simplicissimum
1.1.3.38 510000
-
 gel filtration Penicillium simplicissimum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.38 vanillyl alcohol + O2 Penicillium simplicissimum
-
 vanillin + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.38 Penicillium simplicissimum P56216
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.38 ammonium sulfate precipitation, phenyl Sepharose column chromatography, and hydroxyapatite column chromatography Penicillium simplicissimum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.38 4-ethylphenol + O2 + H2O
-
 Penicillium simplicissimum ? + H2O2
-
 ?
1.1.3.38 eugenol + O2 + H2O
-
 Penicillium simplicissimum coniferyl alcohol + H2O2
-
 ?
1.1.3.38 vanillyl alcohol + O2
-
 Penicillium simplicissimum vanillin + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.38 homooctamer 8 * 61000, SDS-PAGE Penicillium simplicissimum
1.1.3.38 homooctamer 8 * 64000, calculated from amino acid sequence Penicillium simplicissimum

Synonyms

EC Number Synonyms Comment Organism
1.1.3.38 VAO
-
 Penicillium simplicissimum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.38 54.6
-
 melting temperature Penicillium simplicissimum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.38 2.6
-
 Vanillyl alcohol wild type enzyme, at pH 7.5 and 25°C Penicillium simplicissimum
1.1.3.38 2.6
-
 4-Ethylphenol wild type enzyme, at pH 7.5 and 25°C Penicillium simplicissimum
1.1.3.38 11
-
 eugenol wild type enzyme, at pH 7.5 and 25°C Penicillium simplicissimum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.38 FAD
-
 Penicillium simplicissimum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.3.38 17
-
 Vanillyl alcohol wild type enzyme, at pH 7.5 and 25°C Penicillium simplicissimum
1.1.3.38 217
-
 4-Ethylphenol wild type enzyme, at pH 7.5 and 25°C Penicillium simplicissimum
1.1.3.38 2821
-
 eugenol wild type enzyme, at pH 7.5 and 25°C Penicillium simplicissimum