BRENDA - Enzyme Database

The oxygen-tolerant and NAD+-dependent formate dehydrogenase from Rhodobacter capsulatus is able to catalyze the reduction of CO2 to formate

Hartmann, T.; Leimkuehler, S.; FEBS J. 280, 6083-6096 (2013)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.17.1.9
additional information
not inactivated by molecular oxygen
Rhodobacter capsulatus
Cloned(Commentary)
EC Number
Commentary
Organism
1.17.1.9
expressed in Escherichia coli BL21(DE3) cells
Rhodobacter capsulatus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.17.1.9
0.173
-
NAD+
at pH 9.0 and 30°C
Rhodobacter capsulatus
1.17.1.9
0.277
-
formate
with 2,6-dichlorophenolindophenol as cosubstrate, at pH 9.0 and 30°C
Rhodobacter capsulatus
1.17.1.9
0.278
-
formate
with ferricyanide as cosubstrate, at pH 9.0 and 30°C
Rhodobacter capsulatus
1.17.1.9
0.281
-
formate
with NAD+ as cosubstrate, at pH 9.0 and 30°C
Rhodobacter capsulatus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.17.1.9
cytoplasm
-
Rhodobacter capsulatus
5737
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.17.1.9
15000
-
-
Rhodobacter capsulatus
1.17.1.9
50000
-
-
Rhodobacter capsulatus
1.17.1.9
100000
-
-
Rhodobacter capsulatus
1.17.1.9
340000
-
gel filtration
Rhodobacter capsulatus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.17.1.9
formate + NAD+
Rhodobacter capsulatus
-
CO2 + NADH
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.17.1.9
Rhodobacter capsulatus
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.1.9
formate + 2,6-dichlorophenolindophenol
-
740431
Rhodobacter capsulatus
CO2 + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.17.1.9
formate + ferricyanide
-
740431
Rhodobacter capsulatus
CO2 + ferrocyanide
-
-
-
?
1.17.1.9
formate + NAD+
-
740431
Rhodobacter capsulatus
CO2 + NADH
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.17.1.9
heterotrimer
2 * 100000 + 2 * 50000 + 2 * 15000, SDS-PAGE
Rhodobacter capsulatus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.17.1.9
40
-
-
Rhodobacter capsulatus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.17.1.9
1.5
-
CO2
with NADH as cosubstrate, at pH 9.0 and 30°C
Rhodobacter capsulatus
1.17.1.9
32
-
formate
with 2,6-dichlorophenolindophenol as cosubstrate, at pH 9.0 and 30°C
Rhodobacter capsulatus
1.17.1.9
36.5
-
formate
with NAD+ as cosubstrate, at pH 9.0 and 30°C
Rhodobacter capsulatus
1.17.1.9
39.7
-
formate
with ferricyanide as cosubstrate, at pH 9.0 and 30°C
Rhodobacter capsulatus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.17.1.9
9
-
-
Rhodobacter capsulatus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.17.1.9
bis-molybdopterin guanine dinucleotide
-
Rhodobacter capsulatus
1.17.1.9
FMN
-
Rhodobacter capsulatus
1.17.1.9
NAD+
-
Rhodobacter capsulatus
1.17.1.9
[2Fe-2S]-center
-
Rhodobacter capsulatus
1.17.1.9
[4Fe-4S]-center
-
Rhodobacter capsulatus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.17.1.9
additional information
not inactivated by molecular oxygen
Rhodobacter capsulatus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.17.1.9
expressed in Escherichia coli BL21(DE3) cells
Rhodobacter capsulatus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.17.1.9
bis-molybdopterin guanine dinucleotide
-
Rhodobacter capsulatus
1.17.1.9
FMN
-
Rhodobacter capsulatus
1.17.1.9
NAD+
-
Rhodobacter capsulatus
1.17.1.9
[2Fe-2S]-center
-
Rhodobacter capsulatus
1.17.1.9
[4Fe-4S]-center
-
Rhodobacter capsulatus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.17.1.9
0.173
-
NAD+
at pH 9.0 and 30°C
Rhodobacter capsulatus
1.17.1.9
0.277
-
formate
with 2,6-dichlorophenolindophenol as cosubstrate, at pH 9.0 and 30°C
Rhodobacter capsulatus
1.17.1.9
0.278
-
formate
with ferricyanide as cosubstrate, at pH 9.0 and 30°C
Rhodobacter capsulatus
1.17.1.9
0.281
-
formate
with NAD+ as cosubstrate, at pH 9.0 and 30°C
Rhodobacter capsulatus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.17.1.9
cytoplasm
-
Rhodobacter capsulatus
5737
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.17.1.9
15000
-
-
Rhodobacter capsulatus
1.17.1.9
50000
-
-
Rhodobacter capsulatus
1.17.1.9
100000
-
-
Rhodobacter capsulatus
1.17.1.9
340000
-
gel filtration
Rhodobacter capsulatus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.17.1.9
formate + NAD+
Rhodobacter capsulatus
-
CO2 + NADH
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.1.9
formate + 2,6-dichlorophenolindophenol
-
740431
Rhodobacter capsulatus
CO2 + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.17.1.9
formate + ferricyanide
-
740431
Rhodobacter capsulatus
CO2 + ferrocyanide
-
-
-
?
1.17.1.9
formate + NAD+
-
740431
Rhodobacter capsulatus
CO2 + NADH
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.17.1.9
heterotrimer
2 * 100000 + 2 * 50000 + 2 * 15000, SDS-PAGE
Rhodobacter capsulatus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.17.1.9
40
-
-
Rhodobacter capsulatus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.17.1.9
1.5
-
CO2
with NADH as cosubstrate, at pH 9.0 and 30°C
Rhodobacter capsulatus
1.17.1.9
32
-
formate
with 2,6-dichlorophenolindophenol as cosubstrate, at pH 9.0 and 30°C
Rhodobacter capsulatus
1.17.1.9
36.5
-
formate
with NAD+ as cosubstrate, at pH 9.0 and 30°C
Rhodobacter capsulatus
1.17.1.9
39.7
-
formate
with ferricyanide as cosubstrate, at pH 9.0 and 30°C
Rhodobacter capsulatus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.17.1.9
9
-
-
Rhodobacter capsulatus