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Literature summary extracted from

  • Hartmann, T.; Leimkuehler, S.
    The oxygen-tolerant and NAD+-dependent formate dehydrogenase from Rhodobacter capsulatus is able to catalyze the reduction of CO2 to formate (2013), FEBS J., 280, 6083-6096.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.1.9 additional information not inactivated by molecular oxygen Rhodobacter capsulatus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.1.9 expressed in Escherichia coli BL21(DE3) cells Rhodobacter capsulatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.9 0.173
-
 NAD+ at pH 9.0 and 30°C Rhodobacter capsulatus
1.17.1.9 0.277
-
 formate with 2,6-dichlorophenolindophenol as cosubstrate, at pH 9.0 and 30°C Rhodobacter capsulatus
1.17.1.9 0.278
-
 formate with ferricyanide as cosubstrate, at pH 9.0 and 30°C Rhodobacter capsulatus
1.17.1.9 0.281
-
 formate with NAD+ as cosubstrate, at pH 9.0 and 30°C Rhodobacter capsulatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.17.1.9 cytoplasm
-
 Rhodobacter capsulatus 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.9 15000
-
-
 Rhodobacter capsulatus
1.17.1.9 50000
-
-
 Rhodobacter capsulatus
1.17.1.9 100000
-
-
 Rhodobacter capsulatus
1.17.1.9 340000
-
 gel filtration Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.9 formate + NAD+ Rhodobacter capsulatus
-
 CO2 + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.9 Rhodobacter capsulatus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.9 formate + 2,6-dichlorophenolindophenol
-
 Rhodobacter capsulatus CO2 + reduced 2,6-dichlorophenolindophenol
-
 ?
1.17.1.9 formate + ferricyanide
-
 Rhodobacter capsulatus CO2 + ferrocyanide
-
 ?
1.17.1.9 formate + NAD+
-
 Rhodobacter capsulatus CO2 + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.1.9 heterotrimer 2 * 100000 + 2 * 50000 + 2 * 15000, SDS-PAGE Rhodobacter capsulatus

Synonyms

EC Number Synonyms Comment Organism
1.17.1.9 FDH
-
 Rhodobacter capsulatus
1.17.1.9 NAD+-dependent formate dehydrogenase
-
 Rhodobacter capsulatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.1.9 40
-
-
 Rhodobacter capsulatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.17.1.9 1.5
-
 CO2 with NADH as cosubstrate, at pH 9.0 and 30°C Rhodobacter capsulatus
1.17.1.9 32
-
 formate with 2,6-dichlorophenolindophenol as cosubstrate, at pH 9.0 and 30°C Rhodobacter capsulatus
1.17.1.9 36.5
-
 formate with NAD+ as cosubstrate, at pH 9.0 and 30°C Rhodobacter capsulatus
1.17.1.9 39.7
-
 formate with ferricyanide as cosubstrate, at pH 9.0 and 30°C Rhodobacter capsulatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.1.9 9
-
-
 Rhodobacter capsulatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.9 bis-molybdopterin guanine dinucleotide
-
 Rhodobacter capsulatus
1.17.1.9 FMN
-
 Rhodobacter capsulatus
1.17.1.9 NAD+
-
 Rhodobacter capsulatus
1.17.1.9 [2Fe-2S]-center
-
 Rhodobacter capsulatus
1.17.1.9 [4Fe-4S]-center
-
 Rhodobacter capsulatus