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Literature summary extracted from

  • Gonaus, C.; Maresch, D.; Schropp, K.; O Conghaile, P.; Leech, D.; Gorton, L.; Peterbauer, C.K.
    Analysis of Agaricus meleagris pyranose dehydrogenase N-glycosylation sites and performance of partially non-glycosylated enzymes (2017), Enzyme Microb. Technol., 99, 57-66.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.29 expressed in Pichia pastoris X33 cells Leucoagaricus meleagris

Protein Variants

EC Number Protein Variants Comment Organism
1.1.99.29 N175Q N-glycosylation site knock out mutant with reduced activity compares to the wild type enzyme Leucoagaricus meleagris
1.1.99.29 N175Q/N252Q N-glycosylation site knock out mutant with reduced activity compares to the wild type enzyme Leucoagaricus meleagris
1.1.99.29 N252Q N-glycosylation site knock out mutant with reduced activity compares to the wild type enzyme Leucoagaricus meleagris
1.1.99.29 N75G N-glycosylation site knock out mutant with reduced activity compares to the wild type enzyme Leucoagaricus meleagris
1.1.99.29 N75G/N175Q/N252Q N-glycosylation site knock out mutant with reduced activity compares to the wild type enzyme Leucoagaricus meleagris
1.1.99.29 N75G/N252Q N-glycosylation site knock out mutant with reduced activity compares to the wild type enzyme Leucoagaricus meleagris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.29 0.1
-
 ferrocenium ion mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.11
-
 ferrocenium ion mutant enzyme N75G/N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.24
-
 ferrocenium ion mutant enzyme N75G, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.27
-
 ferrocenium ion wild type enzyme, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.29
-
 ferrocenium ion mutant enzyme N75G/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.3
-
 ferrocenium ion mutant enzyme N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.37
-
 ferrocenium ion mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.39
-
 ferrocenium ion mutant enzyme N75Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.58
-
 D-glucose mutant enzyme N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.72
-
 D-glucose mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.8
-
 D-galactose mutant enzyme N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.86
-
 D-galactose mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.87
-
 D-glucose wild type enzyme, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.88
-
 D-glucose mutant enzyme N75G/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.97
-
 D-glucose mutant enzyme N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 1
-
 D-galactose wild type enzyme, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 1.1
-
 D-galactose mutant enzyme N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 1.14
-
 D-galactose mutant enzyme N75G/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 1.5
-
 D-glucose mutant enzyme N75G/N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 1.85
-
 D-glucose mutant enzyme N75G, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 1.94
-
 D-glucose mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 2.19
-
 D-galactose mutant enzyme N75G, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 3.09
-
 D-galactose mutant enzyme N75G/N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 3.55
-
 D-galactose mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 4.74
-
 cellobiose mutant enzyme N75G/N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 5.52
-
 cellobiose mutant enzyme N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 5.7
-
 cellobiose mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 5.72
-
 cellobiose mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 6.38
-
 cellobiose mutant enzyme N75G/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 6.69
-
 cellobiose wild type enzyme, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 7.1
-
 cellobiose mutant enzyme N75G, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 8.13
-
 cellobiose mutant enzyme N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 74.2
-
 D-mannose wild type enzyme, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 83.7
-
 D-mannose mutant enzyme N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 87.9
-
 D-mannose mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 116
-
 D-mannose mutant enzyme N75G/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 142
-
 D-mannose mutant enzyme N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 227
-
 D-mannose mutant enzyme N75G, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 355
-
 D-mannose mutant enzyme N75G/N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 380
-
 D-mannose mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.29 70000
-
 x * 70000, SDS-PAGE Leucoagaricus meleagris

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.29 Leucoagaricus meleagris Q3L245
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.99.29 glycoprotein
-
 Leucoagaricus meleagris

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.29 anion exchange column chromatography and Superdex 75 gel filtration Leucoagaricus meleagris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.29 cellobiose + ferrocenium ion
-
 Leucoagaricus meleagris 2-dehydro-cellobiose + ferrocene
-
 ?
1.1.99.29 D-galactose + ferrocenium ion
-
 Leucoagaricus meleagris 2-dehydro-D-galactose + ferrocene
-
 ?
1.1.99.29 D-glucose + ferrocenium ion
-
 Leucoagaricus meleagris 2-dehydro-D-glucose + ferrocene
-
 ?
1.1.99.29 D-mannose + ferrocenium ion
-
 Leucoagaricus meleagris 2-dehydro-D-mannose + ferrocene
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.29 ? x * 70000, SDS-PAGE Leucoagaricus meleagris

Synonyms

EC Number Synonyms Comment Organism
1.1.99.29 PDH1
-
 Leucoagaricus meleagris
1.1.99.29 pyranose dehydrogenase 1
-
 Leucoagaricus meleagris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.29 3 6 D-glucose mutant enzyme N75G/N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 22.1
-
 D-mannose mutant enzyme N75G, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 25.7
-
 D-galactose mutant enzyme N75G, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 27
-
 D-mannose mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 27.4
-
 D-mannose wild type enzyme, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 29.1
-
 cellobiose mutant enzyme N75G/N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 30.9
-
 cellobiose wild type enzyme, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 31.3
-
 cellobiose mutant enzyme N75G/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 31.5
-
 D-mannose mutant enzyme N75G/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 31.8
-
 D-galactose wild type enzyme, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 33
-
 cellobiose mutant enzyme N75G, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 33.5
-
 D-mannose mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 34.2
-
 D-galactose mutant enzyme N75G/N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 34.7
-
 cellobiose mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 35
-
 D-mannose mutant enzyme N75G/N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 35.2
-
 cellobiose mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 36.6
-
 D-glucose wild type enzyme, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 37.9
-
 D-galactose mutant enzyme N75G/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 38.9
-
 D-galactose mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 39.3
-
 D-galactose mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 40.3
-
 D-galactose mutant enzyme N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 40.8
-
 D-mannose mutant enzyme N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 40.8
-
 D-glucose mutant enzyme N75G/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 41.9
-
 D-mannose mutant enzyme N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 43.8
-
 cellobiose mutant enzyme N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 44.8
-
 ferrocenium ion mutant enzyme N75G/N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 45.3
-
 D-glucose mutant enzyme N75G, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 45.4
-
 D-glucose mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 45.9
-
 D-galactose mutant enzyme N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 48.1
-
 D-glucose mutant enzyme N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 49.7
-
 D-glucose mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 50.7
-
 ferrocenium ion mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 50.8
-
 D-glucose mutant enzyme N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 54.2
-
 ferrocenium ion mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 54.6
-
 ferrocenium ion mutant enzyme N75G/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 55.1
-
 cellobiose mutant enzyme N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 65.8
-
 ferrocenium ion mutant enzyme N75G, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 66.3
-
 ferrocenium ion mutant enzyme N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 87.4
-
 ferrocenium ion wild type enzyme, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 125
-
 ferrocenium ion mutant enzyme N75Q, at pH 7.5 and 30°C Leucoagaricus meleagris

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.29 FAD
-
 Leucoagaricus meleagris