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Literature summary extracted from

  • Fukuda, Y.; Sakuraba, H.; Araki, T.; Ohshima, T.; Yoneda, K.
    Catalytic properties and crystal structure of thermostable NAD(P)H-dependent carbonyl reductase from the hyperthermophilic archaeon Aeropyrum pernix K1 (2016), Enzyme Microb. Technol., 91, 17-25.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.184 expression in Escherichia coli Aeropyrum pernix

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.184 three-dimensional strcuture determined by molecular replacement at a resolution of 2.09 A, in the presence of NADPH. Extensive formation of the intrasubunit ion pair networks, and the presence of the strong intersubunit interaction, is likely responsible for thermostability Aeropyrum pernix

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.184 0.0145
-
 9,10-phenanthrenequinone pH 6.0, 50°C Aeropyrum pernix
1.1.1.184 0.116
-
 NADPH pH 6.0, 50°C Aeropyrum pernix
1.1.1.184 0.176
-
 p-toluquinone pH 6.0, 50°C Aeropyrum pernix
1.1.1.184 0.7
-
 ethyl 4-chloro-3-oxobutanoate pH 6.0, 50°C Aeropyrum pernix

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.184 Aeropyrum pernix Q9Y8Y1 cf. EC 1.1.1.100
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.184 9,10-phenanthrenequinone + NADPH + H+
-
 Aeropyrum pernix ? + NADP+
-
 ?
1.1.1.184 ethyl 4-chloro-3-oxobutanoate + NADPH + H+
-
 Aeropyrum pernix ethyl (3S)-4-chloro-3-hydroxybutanoate + NADP+
-
 ?
1.1.1.184 isatin + NADPH + H+
-
 Aeropyrum pernix 3-hydroxy-2-oxoindole + NADP+
-
 ?
1.1.1.184 p-toluquinone + NADPH
-
 Aeropyrum pernix ? + NADP+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.184 70
-
 above 70°C Aeropyrum pernix

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.184 90
-
 10 min, 75% residual activity Aeropyrum pernix

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.184 1.05
-
 ethyl 4-chloro-3-oxobutanoate pH 6.0, 50°C Aeropyrum pernix
1.1.1.184 1.27
-
 9,10-phenanthrenequinone pH 6.0, 50°C Aeropyrum pernix
1.1.1.184 2.5
-
 p-toluquinone pH 6.0, 50°C Aeropyrum pernix

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.184 6
-
-
 Aeropyrum pernix

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.184 3 11.5
-
 Aeropyrum pernix