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Literature summary extracted from

  • Sulej, J.; Janusz, G.; Osinska-Jaroszuk, M.; Malek, P.; Mazur, A.; Komaniecka, I.; Choma, A.; Rogalski, J.
    Characterization of cellobiose dehydrogenase and its FAD-domain from the ligninolytic basidiomycete Pycnoporus sanguineus (2013), Enzyme Microb. Technol., 53, 427-437.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.99.18 Co2+ 91% residual activity at 100 mM Trametes sanguinea
1.1.99.18 Cu2+ 4% residual activity at 100 mM Trametes sanguinea
1.1.99.18 CuSO4 86% residual activity at 100 mM Trametes sanguinea
1.1.99.18 EDTA 59% residual activity at 100 mM Trametes sanguinea
1.1.99.18 imidazole 48% residual activity at 100 mM Trametes sanguinea
1.1.99.18 KCN 28% residual activity at 100 mM Trametes sanguinea
1.1.99.18 MgSO4 95% residual activity at 100 mM Trametes sanguinea
1.1.99.18 MnSO4 88% residual activity at 100 mM Trametes sanguinea
1.1.99.18 additional information not inhibited by NaN3, NaBr, NaCl, MgCl2, MnCl2, Na2SO4, and ZnSO4 Trametes sanguinea
1.1.99.18 NaF 55% residual activity at 100 mM Trametes sanguinea
1.1.99.18 NaNO3 92% residual activity at 100 mM Trametes sanguinea
1.1.99.18 potassium fluoride 63% residual activity at 100 mM Trametes sanguinea
1.1.99.18 potassium iodide 79% residual activity at 100 mM Trametes sanguinea
1.1.99.18 SDS 5% residual activity at 100 mM Trametes sanguinea
1.1.99.18 Zn2+ 86% residual activity at 100 mM Trametes sanguinea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.18 0.056
-
 cellobiose at pH 4.5 and 30°C Trametes sanguinea
1.1.99.18 0.73
-
 lactose at pH 4.5 and 30°C Trametes sanguinea
1.1.99.18 60.7
-
 maltose at pH 4.5 and 30°C Trametes sanguinea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.18 113400
-
-
 Trametes sanguinea

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.18 Trametes sanguinea S5RVR8
-
-
1.1.99.18 Trametes sanguinea FCL199 S5RVR8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.18 ammonium sulfate precipitation, DEAE-Sepharose column chromatography, phenyl-Sepharose column chromatography, and Sephadex G-50 gel filtration Trametes sanguinea

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.18 15.5
-
 culture filtrate, with lactose and cytochrome c as substrates, at pH 4.5 and 30°C Trametes sanguinea
1.1.99.18 32.3
-
 culture filtrate, with lactose and 2,6-dichlorophenolindophenol as substrates, at pH 4.5 and 30°C Trametes sanguinea
1.1.99.18 1416
-
 after 91.4fold purification, with lactose and cytochrome c as substrates, at pH 4.5 and 30°C Trametes sanguinea
1.1.99.18 1770
-
 after 54.7fold purification, with lactose and ,6-dichlorophenolindophenol as substrates, at pH 4.5 and 30°C Trametes sanguinea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.18 cellobiose + 2,6-dichlorophenolindophenol
-
 Trametes sanguinea cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.18 cellobiose + 2,6-dichlorophenolindophenol
-
 Trametes sanguinea FCL199 cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenolindophenol
-
 Trametes sanguinea lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenolindophenol
-
 Trametes sanguinea FCL199 lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.18 lactose + cytochrome c
-
 Trametes sanguinea lactono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 lactose + cytochrome c
-
 Trametes sanguinea FCL199 lactono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 maltose + 2,6-dichlorophenolindophenol
-
 Trametes sanguinea maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.18 maltose + 2,6-dichlorophenolindophenol
-
 Trametes sanguinea FCL199 maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.18 ? x * 113400, calculated from amino acid sequence Trametes sanguinea

Synonyms

EC Number Synonyms Comment Organism
1.1.99.18 Cdh
-
 Trametes sanguinea
1.1.99.18 CDH1
-
 Trametes sanguinea
1.1.99.18 cellobiose (acceptor) 1-oxidoreductase
-
 Trametes sanguinea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.99.18 50
-
 with 2,6-dichlorophenolindophenol as cosubstrate Trametes sanguinea
1.1.99.18 70
-
 with cytochrome c as cosubstrate Trametes sanguinea

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.99.18 50 80 more than 50% activity between 50 and 80°C Trametes sanguinea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.18 4.5
-
-
 Trametes sanguinea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.99.18 3 6 more than 50% activity between pH 3.0 and 6.0 Trametes sanguinea

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.18 FAD
-
 Trametes sanguinea
1.1.99.18 heme
-
 Trametes sanguinea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.99.18 Trametes sanguinea isoelectric focusing
-
 4.2