BRENDA - Enzyme Database

A blueprint of ectoine metabolism from the genome of the industrial producer Halomonas elongata DSM 2581 T

Schwibbert, K.; Marin-Sanguino, A.; Bagyan, I.; Heidrich, G.; Lentzen, G.; Seitz, H.; Rampp, M.; Schuster, S.C.; Klenk, H.P.; Pfeiffer, F.; Oesterhelt, D.; Kunte, H.J.; Environ. Microbiol. 13, 1973-1994 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
3.5.4.44
expression in Escherichia coli
Halomonas elongata
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.125
36600
-
x * 36600, calculated
Halomonas elongata
3.5.4.44
44900
-
-
Halomonas elongata
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.5.1.125
Halomonas elongata
E1V7W0
-
-
3.5.1.125
Halomonas elongata DSM 2581
E1V7W0
-
-
3.5.4.44
Halomonas elongata
E1V7W1
-
-
3.5.4.44
Halomonas elongata DSM 2581
E1V7W1
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.5.1.125
N2-acetyl-L-2,4-diaminobutanoate + H2O
-
740379
Halomonas elongata
L-2,4-diaminobutanoate + acetate
-
-
-
?
3.5.1.125
N2-acetyl-L-2,4-diaminobutanoate + H2O
-
740379
Halomonas elongata DSM 2581
L-2,4-diaminobutanoate + acetate
-
-
-
?
3.5.4.44
ectoine + H2O
-
740379
Halomonas elongata
N2-acetyl-L-2,4-diaminobutanoate
-
-
-
?
3.5.4.44
ectoine + H2O
-
740379
Halomonas elongata DSM 2581
N2-acetyl-L-2,4-diaminobutanoate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.1.125
?
x * 36600, calculated
Halomonas elongata
3.5.4.44
?
x * 44900, calculated
Halomonas elongata
Synonyms
EC Number
Synonyms
Commentary
Organism
3.5.1.125
doeB
-
Halomonas elongata
3.5.1.125
Helo_3664
-
Halomonas elongata
3.5.1.125
N-alpha-acetyl-L-2,4-diaminobutyric acid deacetylase
-
Halomonas elongata
3.5.4.44
doeA
-
Halomonas elongata
3.5.4.44
Helo_3665
-
Halomonas elongata
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.5.1.125
Halomonas elongata
calculated
-
4.6
3.5.4.44
Halomonas elongata
calculated
-
5
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.44
expression in Escherichia coli
Halomonas elongata
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.125
36600
-
x * 36600, calculated
Halomonas elongata
3.5.4.44
44900
-
-
Halomonas elongata
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.5.1.125
N2-acetyl-L-2,4-diaminobutanoate + H2O
-
740379
Halomonas elongata
L-2,4-diaminobutanoate + acetate
-
-
-
?
3.5.1.125
N2-acetyl-L-2,4-diaminobutanoate + H2O
-
740379
Halomonas elongata DSM 2581
L-2,4-diaminobutanoate + acetate
-
-
-
?
3.5.4.44
ectoine + H2O
-
740379
Halomonas elongata
N2-acetyl-L-2,4-diaminobutanoate
-
-
-
?
3.5.4.44
ectoine + H2O
-
740379
Halomonas elongata DSM 2581
N2-acetyl-L-2,4-diaminobutanoate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.1.125
?
x * 36600, calculated
Halomonas elongata
3.5.4.44
?
x * 44900, calculated
Halomonas elongata
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.5.1.125
Halomonas elongata
calculated
-
4.6
3.5.4.44
Halomonas elongata
calculated
-
5
General Information
EC Number
General Information
Commentary
Organism
3.5.1.125
physiological function
the degradation of ectoine proceeds via hydrolysis of ectoine by DoeA to Nalpha-acetyl-L-2,4-diaminobutanoate, followed by deacetylation to diaminobutanoate by DoeB and a transaminase reaction by DoeD leading to aspartate-semialdehyde. Deletion of doeB result in a mutant that cannot utilize ectoine as carbon and nitrogen source. Nalpha-acetyl-L-2,4-diaminobutanoate accumulates as the predominant amino-reactive solute in the mutant while no Nalpha-acetyl-L-2,4-diaminobutanoate is detectable in the wild type
Halomonas elongata
3.5.4.44
physiological function
the degradation of ectoine proceeds via hydrolysis of ectoine by DoeA to Nalpha-acetyl-L-2,4-diaminobutanoate, followed by deacetylation to diaminobutanoate by DoeB and a transaminase reaction by DoeD leading to aspartate-semialdehyde. Deletion of doeA results in a mutant that cannot utilize ectoine as carbon and nitrogen source
Halomonas elongata
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.5.1.125
physiological function
the degradation of ectoine proceeds via hydrolysis of ectoine by DoeA to Nalpha-acetyl-L-2,4-diaminobutanoate, followed by deacetylation to diaminobutanoate by DoeB and a transaminase reaction by DoeD leading to aspartate-semialdehyde. Deletion of doeB result in a mutant that cannot utilize ectoine as carbon and nitrogen source. Nalpha-acetyl-L-2,4-diaminobutanoate accumulates as the predominant amino-reactive solute in the mutant while no Nalpha-acetyl-L-2,4-diaminobutanoate is detectable in the wild type
Halomonas elongata
3.5.4.44
physiological function
the degradation of ectoine proceeds via hydrolysis of ectoine by DoeA to Nalpha-acetyl-L-2,4-diaminobutanoate, followed by deacetylation to diaminobutanoate by DoeB and a transaminase reaction by DoeD leading to aspartate-semialdehyde. Deletion of doeA results in a mutant that cannot utilize ectoine as carbon and nitrogen source
Halomonas elongata