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Literature summary extracted from

  • Iwasa, H.; Ozawa, K.; Sasaki, N.; Kinoshita, N.; Hiratsuka, A.; Yokoyama, K.
    Thermostable FAD-dependent glucose dehydrogenases from thermophilic filamentous fungus Thermoascus aurantiacus (2016), Electrochemistry, 84, 342-348.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.5.9 expressed in Escherichia coli strain BLR(DE3) and Pichia pastoris strain GS115 Thermoascus aurantiacus

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.9 Thermoascus aurantiacus
-
-
-
1.1.5.9 Thermoascus aurantiacus NBRC 6766
-
-
-
1.1.5.9 Thermoascus aurantiacus NBRC 9748
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.5.9 glycoprotein
-
 Thermoascus aurantiacus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.9 2-deoxy-D-glucose + 2,6-dichlorophenolindophenol about 11% activity compared to D-glucose Thermoascus aurantiacus 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 2-deoxy-D-glucose + 2,6-dichlorophenolindophenol about 8.4% activity compared to D-glucose Thermoascus aurantiacus 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 2-deoxy-D-glucose + 2,6-dichlorophenolindophenol about 11% activity compared to D-glucose Thermoascus aurantiacus NBRC 6766 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 2-deoxy-D-glucose + 2,6-dichlorophenolindophenol about 8.4% activity compared to D-glucose Thermoascus aurantiacus NBRC 6766 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 2-deoxy-D-glucose + 2,6-dichlorophenolindophenol about 11% activity compared to D-glucose Thermoascus aurantiacus NBRC 9748 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 2-deoxy-D-glucose + 2,6-dichlorophenolindophenol about 8.4% activity compared to D-glucose Thermoascus aurantiacus NBRC 9748 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-glucose + 2,6-dichlorophenolindophenol 100% activity Thermoascus aurantiacus D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-glucose + 2,6-dichlorophenolindophenol 100% activity Thermoascus aurantiacus NBRC 6766 D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-glucose + 2,6-dichlorophenolindophenol 100% activity Thermoascus aurantiacus NBRC 9748 D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-raffinose + 2,6-dichlorophenolindophenol about 3.2% activity compared to D-glucose Thermoascus aurantiacus ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-raffinose + 2,6-dichlorophenolindophenol about 4.5% activity compared to D-glucose Thermoascus aurantiacus ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-xylose + 2,6-dichlorophenolindophenol about 50% activity compared to D-glucose Thermoascus aurantiacus D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-xylose + 2,6-dichlorophenolindophenol about 40% activity compared to D-glucose Thermoascus aurantiacus D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-xylose + 2,6-dichlorophenolindophenol about 50% activity compared to D-glucose Thermoascus aurantiacus NBRC 6766 D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-xylose + 2,6-dichlorophenolindophenol about 50% activity compared to D-glucose Thermoascus aurantiacus NBRC 9748 D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 maltose + 2,6-dichlorophenolindophenol about 11% activity compared to D-glucose Thermoascus aurantiacus maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 maltose + 2,6-dichlorophenolindophenol about 21% activity compared to D-glucose Thermoascus aurantiacus maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 additional information no activity with D-arabinose, D-cellobiose, D-fructose, D-galactose, lactitol, maltitol, D-mannitol, D-mannose, D-sorbitol, sucrose, and D-trehalose Thermoascus aurantiacus ?
-
 ?
1.1.5.9 additional information no activity with D-arabinose, D-cellobiose, D-fructose, D-galactose, lactitol, maltitol, D-mannitol, D-mannose, D-sorbitol, sucrose, and D-trehalose Thermoascus aurantiacus NBRC 6766 ?
-
 ?
1.1.5.9 additional information no activity with D-arabinose, D-cellobiose, D-fructose, D-galactose, lactitol, maltitol, D-mannitol, D-mannose, D-sorbitol, sucrose, and D-trehalose Thermoascus aurantiacus NBRC 9748 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.5.9 FAD-dependent glucose dehydrogenase
-
 Thermoascus aurantiacus
1.1.5.9 FADGDH
-
 Thermoascus aurantiacus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.5.9 70
-
-
 Thermoascus aurantiacus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.5.9 40 60 after heat treatment at 40-60°C for 15 min, the enzyme maintains its activity close to 100% Thermoascus aurantiacus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.9 5
-
-
 Thermoascus aurantiacus
1.1.5.9 6 7
-
 Thermoascus aurantiacus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.5.9 3 11 the enzyme is stable for 20 h between pH 3.0 and 11.0 Thermoascus aurantiacus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.9 FAD
-
 Thermoascus aurantiacus