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Literature summary extracted from

  • Gregory, R.C.; Hemsworth, G.R.; Turkenburg, J.P.; Hart, S.J.; Walton, P.H.; Davies, G.J.
    Activity, stability and 3-D structure of the Cu(II) form of a chitin-active lytic polysaccharide monooxygenase from Bacillus amyloliquefaciens (2016), Dalton Trans., 45, 16904-16912.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.53 expression in Escherichia coli Bacillus amyloliquefaciens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.99.53 analysis of the copper active site Bacillus amyloliquefaciens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.99.53 Cu2+ Cu(II) binds with KD value 43 nM at pH 5. The coordination geometry around the copper is distorted from axial symmetry Bacillus amyloliquefaciens
1.14.99.53 Zn2+ may substitue for Cu2+. KD value 0.0081 mM at pH 5 Bacillus amyloliquefaciens

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.53 Bacillus amyloliquefaciens
-
-
-
1.14.99.53 Bacillus amyloliquefaciens DSM 7
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.99.53 improved expression and purification protocol using Champion pET-SUMO vector, yielding high purity AA10 with yields in excess of 9 mg of protein per litre of culture Bacillus amyloliquefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.53 alpha-chitin + acceptor + O2
-
 Bacillus amyloliquefaciens oligosaccharide aldonic acids + reduced acceptor + H2O
-
 ?
1.14.99.53 alpha-chitin + acceptor + O2
-
 Bacillus amyloliquefaciens DSM 7 oligosaccharide aldonic acids + reduced acceptor + H2O
-
 ?
1.14.99.53 beta-chitin + acceptor + O2 beta chitin from squid pen, best substrate Bacillus amyloliquefaciens oligosaccharide aldonic acids + reduced acceptor + H2O strong preference towards even-numbered products ?
1.14.99.53 beta-chitin + acceptor + O2 beta chitin from squid pen, best substrate Bacillus amyloliquefaciens DSM 7 oligosaccharide aldonic acids + reduced acceptor + H2O strong preference towards even-numbered products ?

Synonyms

EC Number Synonyms Comment Organism
1.14.99.53 AA10
-
 Bacillus amyloliquefaciens
1.14.99.53 RBAM17540
-
 Bacillus amyloliquefaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.99.53 additional information
-
 chitin binding increases the thermal stability of the enzyme by 8.3 degrees, copper binding increases melting temperature by 21.6 degrees. Addition of chitin to the copper-bound enzyme increases thermal stability by additional 3.5 degrees Bacillus amyloliquefaciens
1.14.99.53 68.7
-
 melting temperature, presence of both copper and chitin Bacillus amyloliquefaciens