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Literature summary extracted from

  • Li, C.; Tao, F.; Xu, P.
    Carbon flux trapping: highly efficient production of polymer-grade D-lactic acid with a thermophilic D-lactate dehydrogenase (2016), ChemBioChem, 17, 1491-1494.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.28 synthesis D-LDH is a candidate for thermophilic D-lactic acid production Thermodesulfatator indicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.28 gene WP_013906894 , recombinant expression of the enzyme in Bacillus licheniformis strain ATCC 14580 MW3 derivative BL2 with a deleted native gene ldh, encoding L-LDH. Gene WP_013906894 is introduced to redirect the carbon flux, the genes for 2,3-butanediol production, alsS and alsD, are replaced with WP_013906894, giving strains BN11, BN22, BN44, and BN66 Thermodesulfatator indicus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.28 additional information fermentation conditions are critical for microbial growth and lactic acid secretion, optimization of enzyme and D-lactic acid production by the enzyme from Thermodesulfatator indicus in Bacillus licheniformis strains B11 and BA11, overview. Disruption of the lactate permease LctP in host strains BN11 and BA11 results in reductions in D-lactic acid (28.6 and 35.2%, respectively). Replacement of LctP inhost strain BN11 with the mesophilic permease LldP of Escherichia coli also leads to a 12.1% decrease in D-lactic acid accumulation Thermodesulfatator indicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.28 Co2+ activates Thermodesulfatator indicus
1.1.1.28 Zn2+ activates Thermodesulfatator indicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.28 pyruvate + NADH + H+ Thermodesulfatator indicus
-
 (R)-lactate + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.28 Thermodesulfatator indicus F8A9V0
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.28 30.2
-
 pyruvate reduction, pH 6.0, 70°C Thermodesulfatator indicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.28 glyoxylate + NADH + H+
-
 Thermodesulfatator indicus hydroxyacetate + NAD+
-
 r
1.1.1.28 additional information no activity towards L-lactate Thermodesulfatator indicus ?
-
 ?
1.1.1.28 oxaloacetate + NADH + H+
-
 Thermodesulfatator indicus D-malate + NAD+
-
 r
1.1.1.28 phenylpyruvate + NADH + H+
-
 Thermodesulfatator indicus D-phenyllactate + NAD+
-
 r
1.1.1.28 pyruvate + NADH + H+
-
 Thermodesulfatator indicus (R)-lactate + NAD+
-
 ?
1.1.1.28 pyruvate + NADH + H+ preferred subtrate Thermodesulfatator indicus (R)-lactate + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.28 tetramer
-
 Thermodesulfatator indicus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.28 D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein
-
 Thermodesulfatator indicus
1.1.1.28 D-LDH
-
 Thermodesulfatator indicus
1.1.1.28 D-LDH-like enzyme
-
 Thermodesulfatator indicus
1.1.1.28 LdhTi
-
 Thermodesulfatator indicus
1.1.1.28 WP_013906894
-
 Thermodesulfatator indicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.28 70
-
-
 Thermodesulfatator indicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.28 30 100 activity range, high activity at 60-80°C Thermodesulfatator indicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.28 6
-
-
 Thermodesulfatator indicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.28 3 11 activity range Thermodesulfatator indicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.28 NADH
-
 Thermodesulfatator indicus