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Literature summary extracted from

  • Xu, G.C.; Shang, Y.P.; Yu, H.L.; Xu, J.H.
    Identification of key residues in Debaryomyces hansenii carbonyl reductase for highly productive preparation of (S)-aryl halohydrins (2015), Chem. Commun. (Camb.), 51, 15728-15731.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.B3 homology modeling of structure. The substrate binding pocket consists of residues P122, W123, E125, S174, N179, V180, S214, P215, G217, Y218, I223, S224, D225, F226 and V227, with all of the residues lying in the flexible loop regions Debaryomyces hansenii

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.B3 N179S about 40fold increase in specific activity Debaryomyces hansenii
1.1.1.B3 N179S/I214F/S215G about 50fold increase in specific activity Debaryomyces hansenii
1.1.1.B3 S174G about 7fold increase in specific activity Debaryomyces hansenii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.B3 0.4
-
 1-chloroacetophenone mutant N179S, pH 6.5, 30°C Debaryomyces hansenii
1.1.1.B3 1.31
-
 1-chloroacetophenone mutant S174G, pH 6.5, 30°C Debaryomyces hansenii
1.1.1.B3 1.45
-
 1-chloroacetophenone mutant N179S/I214F/S215G, pH 6.5, 30°C Debaryomyces hansenii
1.1.1.B3 2.25
-
 1-chloroacetophenone wild-type, pH 6.5, 30°C Debaryomyces hansenii

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.B3 Debaryomyces hansenii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.B3 1-chloroacetophenone + NADH + H+
-
 Debaryomyces hansenii (1S)-2-chloro-1-phenylethanol + NAD+ more thasn 99% enantiomeric excess ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.B3 3.95
-
 1-chloroacetophenone wild-type, pH 6.5, 30°C Debaryomyces hansenii
1.1.1.B3 21.8
-
 1-chloroacetophenone mutant S174G, pH 6.5, 30°C Debaryomyces hansenii
1.1.1.B3 119
-
 1-chloroacetophenone mutant N179S, pH 6.5, 30°C Debaryomyces hansenii
1.1.1.B3 204
-
 1-chloroacetophenone mutant N179S/I214F/S215G, pH 6.5, 30°C Debaryomyces hansenii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.B3 1.75
-
 1-chloroacetophenone wild-type, pH 6.5, 30°C Debaryomyces hansenii
1.1.1.B3 14.2
-
 1-chloroacetophenone mutant S174G, pH 6.5, 30°C Debaryomyces hansenii
1.1.1.B3 140
-
 1-chloroacetophenone mutant N179S/I214F/S215G, pH 6.5, 30°C Debaryomyces hansenii
1.1.1.B3 295
-
 1-chloroacetophenone mutant N179S, pH 6.5, 30°C Debaryomyces hansenii