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Literature summary extracted from

  • Brown, S.; Meredith, T.; Swoboda, J.; Walker, S.
    Staphylococcus aureus and Bacillus subtilis W23 make polyribitol wall teichoic acids using different enzymatic pathways (2010), Chem. Biol., 17, 1101-1110.
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.46 CDP-ribitol + 4-O-[(2R)-1-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol Bacillus spizizenii
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 CMP + 4-O-[1-D-ribitylphospho-(2R)-1-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.7.8.46 CDP-ribitol + 4-O-[(2R)-1-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol Bacillus spizizenii ATCC 23059
-
 CMP + 4-O-[1-D-ribitylphospho-(2R)-1-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.7.8.46 additional information Bacillus spizizenii TarK functions to prime the wall teichoic acid intermediate for chain extension by TarL. TarK is only able to accept lipid-diphospho-GlcNAc-ManNAc-GroP, the TagB product, as a substrate ?
-
 ?
2.7.8.46 additional information Bacillus spizizenii ATCC 23059 TarK functions to prime the wall teichoic acid intermediate for chain extension by TarL. TarK is only able to accept lipid-diphospho-GlcNAc-ManNAc-GroP, the TagB product, as a substrate ?
-
 ?
2.7.8.47 n CDP-ribitol + 4-O-[1-D-ribitylphospho-(2R)-1-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol Bacillus subtilis
-
 n CMP + 4-O-[(1-D-ribitylphospho)n-(1-D-ribitylphospho)-(2R)-1-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.7.8.47 n CDP-ribitol + 4-O-[1-D-ribitylphospho-(2R)-1-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol Bacillus subtilis W23
-
 n CMP + 4-O-[(1-D-ribitylphospho)n-(1-D-ribitylphospho)-(2R)-1-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.45 Staphylococcus aureus Q2G1C1
-
-
2.7.8.45 Staphylococcus aureus NCTC 8325 Q2G1C1
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-
2.7.8.46 Bacillus spizizenii Q8RKJ1
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-
2.7.8.46 Bacillus spizizenii ATCC 23059 Q8RKJ1
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-
2.7.8.47 Bacillus subtilis Q8RKJ2
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-
2.7.8.47 Bacillus subtilis W23 Q8RKJ2
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-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.45 CDP-glycerol + 4-O-[(2R)-1-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 Staphylococcus aureus CDP + 4-O-di[(2R)-1-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol transfer of a single glycerol-phosphate unit. Under high enzyme and substrate concentrations for prolonged reaction times, an additional product containing three glycerol-phosphate units can also be observed ?
2.7.8.45 CDP-glycerol + 4-O-[(2R)-1-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 Staphylococcus aureus NCTC 8325 CDP + 4-O-di[(2R)-1-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol transfer of a single glycerol-phosphate unit. Under high enzyme and substrate concentrations for prolonged reaction times, an additional product containing three glycerol-phosphate units can also be observed ?
2.7.8.46 CDP-ribitol + 4-O-[(2R)-1-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 Bacillus spizizenii CMP + 4-O-[1-D-ribitylphospho-(2R)-1-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.7.8.46 CDP-ribitol + 4-O-[(2R)-1-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 Bacillus spizizenii ATCC 23059 CMP + 4-O-[1-D-ribitylphospho-(2R)-1-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.7.8.46 additional information TarK functions to prime the wall teichoic acid intermediate for chain extension by TarL. TarK is only able to accept lipid-diphospho-GlcNAc-ManNAc-GroP, the TagB product, as a substrate Bacillus spizizenii ?
-
 ?
2.7.8.46 additional information TarK functions to prime the wall teichoic acid intermediate for chain extension by TarL. TarK is only able to accept lipid-diphospho-GlcNAc-ManNAc-GroP, the TagB product, as a substrate Bacillus spizizenii ATCC 23059 ?
-
 ?
2.7.8.47 n CDP-ribitol + 4-O-[1-D-ribitylphospho-(2R)-1-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 Bacillus subtilis n CMP + 4-O-[(1-D-ribitylphospho)n-(1-D-ribitylphospho)-(2R)-1-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.7.8.47 n CDP-ribitol + 4-O-[1-D-ribitylphospho-(2R)-1-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 Bacillus subtilis W23 n CMP + 4-O-[(1-D-ribitylphospho)n-(1-D-ribitylphospho)-(2R)-1-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.8.46 tarK
-
 Bacillus spizizenii

General Information

EC Number General Information Comment Organism
2.7.8.45 physiological function Staphylococcus aureus requires a glycerol-phosphate primase called TarF in order make polyribitol-phosphate-wall teichoic acids. TarF is essentisal in wild-type background but can be deleted in a strain expression Bacillus subtilis TarK-TarL Staphylococcus aureus
2.7.8.47 physiological function polymerase TarL catalyzes chain extension of wall teichoic acid intermediates primed by TarF. TarL is unable to use glycerol-phosphate primed intermediates to create the polymer, but TarLBs reacts with the ribitol-phosphate-primed substrate and adds multiple polyribitolphosphate subunits Bacillus subtilis