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Literature summary extracted from

  • Yu, Y.; Liu, C.; Wang, B.; Li, Y.; Zhang, H.
    Characterization of 3,17beta-hydroxysteroid dehydrogenase in Comamonas testosteroni (2015), Chem. Biol. Interact., 234, 221-228.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.51 gene betahsd, sequence comparisons, cloning and location of the TSS and promoter (126 bp) of the 3,17beta-HSD gene, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3) Comamonas testosteroni

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.51 additional information construction of a betahsd knockout mutant of Comamonas testosteroni by homologous integration, and preparation of 3,17b-HSD gene high expression mutant Comamonas testosteroni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.51 additional information Comamonas testosteroni the wild-type strain ATCC 119960 degrades steroids, such as testosterone, estradiol, or cholesterol, differences in the enzyme mutant strains, overview ?
-
 ?
1.1.1.51 additional information Comamonas testosteroni ATCC 119960 the wild-type strain ATCC 119960 degrades steroids, such as testosterone, estradiol, or cholesterol, differences in the enzyme mutant strains, overview ?
-
 ?
1.1.1.51 testosterone + NADP+ Comamonas testosteroni
-
 androstenedione + NADPH + H+
-
 r
1.1.1.51 testosterone + NADP+ Comamonas testosteroni ATCC 119960
-
 androstenedione + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.51 Comamonas testosteroni P19871
-
-
1.1.1.51 Comamonas testosteroni ATCC 119960 P19871
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.51 additional information the wild-type strain ATCC 119960 degrades steroids, such as testosterone, estradiol, or cholesterol, differences in the enzyme mutant strains, overview Comamonas testosteroni ?
-
 ?
1.1.1.51 additional information the wild-type strain ATCC 119960 degrades steroids, such as testosterone, estradiol, or cholesterol, differences in the enzyme mutant strains, overview Comamonas testosteroni ATCC 119960 ?
-
 ?
1.1.1.51 testosterone + NADP+
-
 Comamonas testosteroni androstenedione + NADPH + H+
-
 r
1.1.1.51 testosterone + NADP+
-
 Comamonas testosteroni ATCC 119960 androstenedione + NADPH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.51 3,17beta-HSD
-
 Comamonas testosteroni
1.1.1.51 3,17beta-hydroxysteroid dehydrogenase
-
 Comamonas testosteroni
1.1.1.51 betahsd
-
 Comamonas testosteroni

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.51 NADP+
-
 Comamonas testosteroni
1.1.1.51 NADPH
-
 Comamonas testosteroni

Expression

EC Number Organism Comment Expression
1.1.1.51 Comamonas testosteroni 3,17beta-HSD gene expression is induced by testosterone, but not by estradiol and cholesterol up

General Information

EC Number General Information Comment Organism
1.1.1.51 malfunction compared to the wild-type Comamonas testosteroni, degradation ability of testosterone and cholesterol is almost lost, and degradation of estradiol is decreased in the 3,17beta-HSD knockout mutant. Degradation of testosterone and cholesterol is obviously increased in the 3,17beta-HSD overexpression mutant. The growths in the medium with testosterone, cholesterol or estradiol are impaired in 3,17beta-HSD knockout mutant Comamonas testosteroni
1.1.1.51 metabolism Comamonas testosteroni strain ATCC11996 is a gram-negative bacterium which can use steroids as carbon and energy source. 3,17beta-HSD is an enzyme which is involved in the complete oxidative degradation of the steroid skeleton, induced in the presence of these compounds in the culture medium Comamonas testosteroni